FastQCFastQC Report
Thu 9 Feb 2017
SRR2727306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7496910
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT299480.39947124882118096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98270.1310806719034909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94690.1263053711462456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC39500.017.75063337
TCTTATA69250.016.58989137
AACGCAG130550.016.310615
ACGCAGA149100.014.3185796
TATACCG3500.014.2714285
AGAGTAC157150.013.59688210
CGCAGAG159600.013.4924817
GAGTACT88700.013.41093511
CAGAGTA163300.013.3453769
CTCTTAT109250.013.20823837
AGTACTT92050.012.94296512
GGTATCA86950.012.9361711
TAGATCG3150.012.9206345
TAGTACT6700.012.7014924
CAACGCA167700.012.6091234
TCAACGC169100.012.5484923
GTACTTT94300.012.51643713
GCAGAGT172950.012.5151788
ATCAACG171000.012.3766082
TATCAAC171100.012.3261251
Produced by FastQC (version 0.11.5)