##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727306.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7496910
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13288848338849	33.0	31.0	34.0	30.0	34.0
2	32.28132497255536	34.0	31.0	34.0	30.0	34.0
3	32.35984652343432	34.0	31.0	34.0	30.0	34.0
4	35.93559106351817	37.0	35.0	37.0	35.0	37.0
5	35.81484318205767	37.0	35.0	37.0	35.0	37.0
6	35.827744764176174	37.0	35.0	37.0	35.0	37.0
7	35.77079996958747	37.0	35.0	37.0	35.0	37.0
8	35.742267680951215	37.0	35.0	37.0	35.0	37.0
9	37.46799094560292	39.0	37.0	39.0	35.0	39.0
10	37.3046161685281	39.0	37.0	39.0	34.0	39.0
11	37.40985232582491	39.0	37.0	39.0	35.0	39.0
12	37.311038948046594	39.0	37.0	39.0	34.0	39.0
13	37.36698052931141	39.0	37.0	39.0	34.0	39.0
14	38.545424848370864	40.0	38.0	41.0	34.0	41.0
15	38.4999713215178	40.0	38.0	41.0	34.0	41.0
16	38.433977198605824	40.0	38.0	41.0	34.0	41.0
17	38.38459965505788	40.0	38.0	41.0	34.0	41.0
18	38.32798446293206	40.0	38.0	41.0	34.0	41.0
19	38.3260938173194	40.0	38.0	41.0	34.0	41.0
20	38.2020523122193	40.0	38.0	41.0	34.0	41.0
21	38.11984510951846	40.0	38.0	41.0	33.0	41.0
22	38.03972249900292	40.0	38.0	41.0	33.0	41.0
23	37.92157115398211	40.0	37.0	41.0	33.0	41.0
24	37.80678106046358	40.0	37.0	41.0	32.0	41.0
25	37.7192682051672	40.0	37.0	41.0	32.0	41.0
26	37.34164022243831	39.0	37.0	41.0	31.0	41.0
27	37.11907265793507	39.0	36.0	41.0	31.0	41.0
28	36.95416791718188	39.0	36.0	40.0	30.0	41.0
29	36.913724721251825	39.0	36.0	40.0	30.0	41.0
30	36.82673474804953	39.0	36.0	40.0	30.0	41.0
31	36.613980693379006	39.0	36.0	40.0	30.0	41.0
32	36.49838773574713	39.0	36.0	40.0	30.0	41.0
33	36.310825793560284	39.0	35.0	40.0	29.0	41.0
34	36.16838484122125	39.0	35.0	40.0	29.0	41.0
35	36.010240885911664	39.0	35.0	40.0	28.0	41.0
36	35.85755811394294	38.0	35.0	40.0	27.0	41.0
37	35.61353050790259	38.0	35.0	40.0	26.0	41.0
38	35.49437528261644	38.0	35.0	40.0	26.0	41.0
39	35.32201080178367	38.0	34.0	40.0	25.0	41.0
40	35.186414936287086	38.0	34.0	40.0	25.0	41.0
41	35.04667909845523	38.0	34.0	40.0	24.0	41.0
42	34.851011416703685	38.0	33.0	40.0	24.0	41.0
43	31.355378415907353	35.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	21.0
11	34.0
12	23.0
13	44.0
14	69.0
15	125.0
16	221.0
17	481.0
18	906.0
19	1918.0
20	3920.0
21	7392.0
22	13192.0
23	21028.0
24	31536.0
25	44620.0
26	59605.0
27	78835.0
28	100611.0
29	126802.0
30	157940.0
31	196720.0
32	243027.0
33	303830.0
34	388502.0
35	509271.0
36	707731.0
37	1098010.0
38	1978756.0
39	1421735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83111308525779	18.043460572422504	12.251247513975757	26.874178828343943
2	19.273700764715063	20.63904995524823	33.745356420178446	26.341892859858262
3	18.977778844884092	21.401564111080432	30.15239612053499	29.468260923500484
4	15.282016190670555	15.324353633697083	32.52473352354504	36.86889665208732
5	14.95377428834013	36.489873828017146	33.712502884521754	14.84384899912097
6	37.37573213497294	34.26597091334963	13.72067958665637	14.637617365021056
7	30.99926503052591	30.261627790649747	19.45556235835831	19.28354482046603
8	28.691327493594027	34.314430878855426	18.242649304846932	18.75159232270362
9	27.276677991332427	13.318847365114427	17.503344711354412	41.901129932198735
10	16.07443600096573	27.171354598094418	33.057312946267196	23.696896454672657
11	38.27446241184702	20.437033391090463	20.408074793481582	20.88042940358094
12	22.21794045813542	24.4567028282319	29.08670372193344	24.238652991699247
13	32.52293278163937	18.983514541324357	22.822442846452738	25.671109830583532
14	23.04789573304201	19.45526890412183	24.592665511524082	32.904169851312076
15	26.408573665683594	27.274863910597837	20.791792885335425	25.524769538383147
16	25.856186081999116	25.41768541972626	22.920056396568718	25.8060721017059
17	25.310347863319688	25.55715621502726	24.209654377603574	24.922841544049483
18	25.297796025295753	24.806260179193828	24.627426499717885	25.26851729579253
19	26.287523793136103	24.805139717563637	24.56766854610766	24.339667943192595
20	25.932430828167874	24.558784886039714	24.37481575742539	25.133968528367017
21	25.609271019660103	24.714288953715595	24.40564179108459	25.27079823553971
22	26.28133457651219	24.29753058260003	23.91744065221538	25.503694188672398
23	25.65448964973569	24.362143869941082	24.158233192075134	25.825133288248093
24	25.605135982691536	24.287139634862896	24.174079720844986	25.93364466160058
25	25.84730242193117	24.004476510989196	24.28826009649309	25.85996097058655
26	25.480297882727683	24.320820177913298	24.511618786940222	25.687263152418797
27	25.860254424823026	24.52599804452768	24.223153272481596	25.3905942581677
28	25.69782750493203	24.6386311160198	24.22703487170047	25.436506507347694
29	25.527530675971832	24.827655660798918	24.372107975152428	25.27270568807682
30	25.686876326379803	24.641578997213518	24.172599110833666	25.498945565573017
31	25.654022790723115	24.164208987436155	24.28904708740001	25.89272113444072
32	25.218189894236424	24.539110113366707	24.375029178688287	25.867670813708582
33	25.40560044071491	24.09680788484856	24.419754272093435	26.0778374023431
34	25.634934926523062	24.000848349520002	24.785838432100693	25.578378291856247
35	25.43303841182567	24.048854794842143	24.746795679820085	25.771311113512102
36	25.154070143565814	23.647996307812154	25.211787256349616	25.986146292272416
37	24.992750346476083	23.379525697920876	25.436413135545177	26.19131082005786
38	24.635443135905327	23.378431913948546	25.88955983198411	26.09656511816202
39	24.462585251790404	23.14666976127498	26.409534061366617	25.981210925568
40	24.647621486719196	22.94712088046942	26.625369652296747	25.779887980514637
41	24.125179573984482	23.009226467971473	26.980582666725354	25.885011291318687
42	23.821414422742173	23.05735296275399	26.980876120961838	26.140356493542004
43	23.96204569616015	22.258463820427348	26.904538002990563	26.874952480421932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	400.0
1	469.5
2	539.0
3	1405.0
4	2271.0
5	2271.0
6	2623.5
7	2976.0
8	2990.0
9	3004.0
10	4403.0
11	5802.0
12	5802.0
13	12085.5
14	18369.0
15	31529.5
16	44690.0
17	32878.5
18	21067.0
19	21067.0
20	25778.5
21	30490.0
22	27528.5
23	24567.0
24	31803.0
25	39039.0
26	39039.0
27	49461.5
28	59884.0
29	71975.0
30	84066.0
31	101653.0
32	119240.0
33	119240.0
34	142020.0
35	164800.0
36	194928.5
37	225057.0
38	262839.5
39	300622.0
40	300622.0
41	341870.5
42	383119.0
43	426866.5
44	470614.0
45	516702.5
46	562791.0
47	562791.0
48	606960.0
49	651129.0
50	672714.0
51	694299.0
52	693198.5
53	692098.0
54	692098.0
55	665493.0
56	638888.0
57	605482.0
58	572076.0
59	530102.0
60	488128.0
61	488128.0
62	431166.0
63	374204.0
64	321753.5
65	269303.0
66	226630.5
67	183958.0
68	183958.0
69	153841.5
70	123725.0
71	105057.5
72	86390.0
73	71787.5
74	57185.0
75	57185.0
76	47718.5
77	38252.0
78	31898.5
79	25545.0
80	20780.0
81	16015.0
82	16015.0
83	12756.5
84	9498.0
85	7626.5
86	5755.0
87	4742.0
88	3729.0
89	3729.0
90	2799.5
91	1870.0
92	1377.5
93	885.0
94	628.0
95	371.0
96	371.0
97	255.0
98	139.0
99	100.0
100	61.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7496910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.77229305643727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32456433854452	44.79885141919955
2	10.093164675350598	11.258378762810683
3	3.476708927042342	5.817120874528112
4	1.7038416467845916	3.801086225849317
5	0.9921929464423159	2.76684378887554
6	0.6522649632704386	2.182698760917912
7	0.4623353749969366	1.8049852817280876
8	0.34845915328064836	1.5547492811973052
9	0.2609048544635305	1.3096135802688424
>10	1.533333305373129	15.918799647404953
>50	0.11514304719952562	4.3594791024579065
>100	0.03620198991661124	3.3048018666553824
>500	6.456798177256236E-4	0.23682629187267565
>1k	1.4345851511890612E-4	0.13295011758442968
>5k	7.172925137882103E-5	0.3534579366245366
>10k+	2.390975045960701E-5	0.39935706202488586
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29948	0.39947124882118096	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9827	0.1310806719034909	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9469	0.1263053711462456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3338828930852844E-5
2	0.0	0.0	0.0	0.0	1.3338828930852844E-5
3	0.0	0.0	0.0	0.0	1.3338828930852844E-5
4	0.0	0.0	0.0	0.0	1.3338828930852844E-5
5	0.0	0.0	0.0	0.0	1.3338828930852844E-5
6	0.0	0.0	0.0	0.0	5.335531572341138E-5
7	0.0	0.0	0.0	0.0	6.669414465426423E-5
8	0.0	0.0	0.0	0.0	6.669414465426423E-5
9	0.0	0.0	0.0	0.0	9.337180251596991E-5
10	1.3338828930852844E-5	0.0	0.0	0.0	9.337180251596991E-5
11	1.3338828930852844E-5	0.0	0.0	1.3338828930852844E-5	9.337180251596991E-5
12	1.3338828930852844E-5	0.0	0.0	2.667765786170569E-5	9.337180251596991E-5
13	1.3338828930852844E-5	1.3338828930852844E-5	0.0	5.335531572341138E-5	9.337180251596991E-5
14	1.3338828930852844E-5	1.3338828930852844E-5	0.0	5.335531572341138E-5	1.6006594717023412E-4
15	1.3338828930852844E-5	2.667765786170569E-5	0.0	9.337180251596991E-5	1.6006594717023412E-4
16	2.667765786170569E-5	2.667765786170569E-5	0.0	1.7340477610108698E-4	2.667765786170569E-4
17	4.001648679255853E-5	2.667765786170569E-5	0.0	2.8011540754790975E-4	2.667765786170569E-4
18	4.001648679255853E-5	2.667765786170569E-5	0.0	3.7348721006387963E-4	3.3347072327132113E-4
19	8.003297358511706E-5	2.667765786170569E-5	0.0	5.20214328303261E-4	3.3347072327132113E-4
20	8.003297358511706E-5	2.667765786170569E-5	0.0	7.869909069203178E-4	3.4680955220217397E-4
21	8.003297358511706E-5	2.667765786170569E-5	0.0	0.001280527577361873	4.26842525787291E-4
22	8.003297358511706E-5	2.667765786170569E-5	0.0	0.0020941961421438965	4.26842525787291E-4
23	8.003297358511706E-5	4.001648679255853E-5	0.0	0.0025343774968620406	5.735696440266723E-4
24	8.003297358511706E-5	4.001648679255853E-5	0.0	0.0034014013773674755	6.00247301888378E-4
25	8.003297358511706E-5	4.001648679255853E-5	0.0	0.0038282439031547665	6.135861308192308E-4
26	8.003297358511706E-5	4.001648679255853E-5	0.0	0.004628573639005937	6.135861308192308E-4
27	8.003297358511706E-5	4.001648679255853E-5	0.0	0.00829675159499047	6.135861308192308E-4
28	8.003297358511706E-5	4.001648679255853E-5	0.0	0.030732661856684954	6.135861308192308E-4
29	8.003297358511706E-5	4.001648679255853E-5	0.0	0.08391457280399525	6.135861308192308E-4
30	8.003297358511706E-5	4.001648679255853E-5	0.0	0.16145318537904282	6.135861308192308E-4
31	8.003297358511706E-5	4.001648679255853E-5	0.0	0.43501922792190384	6.269249597500837E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3950	0.0	17.750633	37
TCTTATA	6925	0.0	16.589891	37
AACGCAG	13055	0.0	16.31061	5
ACGCAGA	14910	0.0	14.318579	6
TATACCG	350	0.0	14.271428	5
AGAGTAC	15715	0.0	13.596882	10
CGCAGAG	15960	0.0	13.492481	7
GAGTACT	8870	0.0	13.410935	11
CAGAGTA	16330	0.0	13.345376	9
CTCTTAT	10925	0.0	13.208238	37
AGTACTT	9205	0.0	12.942965	12
GGTATCA	8695	0.0	12.936171	1
TAGATCG	315	0.0	12.920634	5
TAGTACT	670	0.0	12.701492	4
CAACGCA	16770	0.0	12.609123	4
TCAACGC	16910	0.0	12.548492	3
GTACTTT	9430	0.0	12.516437	13
GCAGAGT	17295	0.0	12.515178	8
ATCAACG	17100	0.0	12.376608	2
TATCAAC	17110	0.0	12.326125	1
>>END_MODULE