FastQCFastQC Report
Thu 9 Feb 2017
SRR2727305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4750380
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT426910.8986859998568535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131540.2769041634563972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124520.26212639830918794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100550.21166727714414424No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA54280.11426454304708256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC33500.017.83731337
AACGCAG162400.015.2306035
TCTTATA56750.014.73480137
TATACCG1803.3396645E-914.3888895
GAGTACT109800.014.10245911
AGTACTT111500.013.90403612
CGTATAC1356.576871E-613.7037043
TAGACTG6100.013.3442625
ACGCAGA188100.013.179166
GTACTTT116800.013.14640413
CTCTTAT87700.013.01539437
AGAGTAC191000.012.9510
CGCAGAG192700.012.9221597
TACTTTT119600.012.85409814
GTATAAG4750.012.8526321
CAGAGTA195750.012.8342289
TATACTG5650.012.4424775
CTAGACT5950.012.43697554
TAGACAG7000.012.4214285
GTATTAG4350.012.3333341
Produced by FastQC (version 0.11.5)