##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727304.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4695224
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13458442025343	33.0	31.0	34.0	30.0	34.0
2	32.28229025920808	34.0	31.0	34.0	30.0	34.0
3	32.364227564009724	34.0	31.0	34.0	30.0	34.0
4	35.94426996454269	37.0	35.0	37.0	35.0	37.0
5	35.82142981889682	37.0	35.0	37.0	35.0	37.0
6	35.836005055349865	37.0	35.0	37.0	35.0	37.0
7	35.78418537645914	37.0	35.0	37.0	35.0	37.0
8	35.75798662641016	37.0	35.0	37.0	35.0	37.0
9	37.4847892241137	39.0	37.0	39.0	35.0	39.0
10	37.32426397547806	39.0	37.0	39.0	34.0	39.0
11	37.42873375157394	39.0	37.0	39.0	35.0	39.0
12	37.3333263759088	39.0	37.0	39.0	34.0	39.0
13	37.39094066651559	39.0	37.0	39.0	34.0	39.0
14	38.57505477906911	40.0	38.0	41.0	34.0	41.0
15	38.53634586976042	40.0	38.0	41.0	34.0	41.0
16	38.477848341208	40.0	38.0	41.0	34.0	41.0
17	38.43202901501611	40.0	38.0	41.0	34.0	41.0
18	38.381999240078855	40.0	38.0	41.0	34.0	41.0
19	38.38320109967064	40.0	38.0	41.0	34.0	41.0
20	38.27081924099894	40.0	38.0	41.0	34.0	41.0
21	38.19506204602805	40.0	38.0	41.0	34.0	41.0
22	38.12128324442029	40.0	38.0	41.0	33.0	41.0
23	38.01174470057233	40.0	38.0	41.0	33.0	41.0
24	37.90940091463155	40.0	37.0	41.0	33.0	41.0
25	37.82514508359984	40.0	37.0	41.0	33.0	41.0
26	37.4725063170575	39.0	37.0	41.0	32.0	41.0
27	37.26693444231841	39.0	37.0	41.0	31.0	41.0
28	37.1278215906206	39.0	36.0	40.0	31.0	41.0
29	37.09570618994962	39.0	36.0	40.0	31.0	41.0
30	37.01651593193424	39.0	36.0	40.0	31.0	41.0
31	36.822580562716496	39.0	36.0	40.0	30.0	41.0
32	36.71752785383615	39.0	36.0	40.0	30.0	41.0
33	36.54056164306538	39.0	36.0	40.0	30.0	41.0
34	36.41728701335655	39.0	35.0	40.0	30.0	41.0
35	36.27275738069153	39.0	35.0	40.0	29.0	41.0
36	36.135007403267664	39.0	35.0	40.0	29.0	41.0
37	35.90151950151899	38.0	35.0	40.0	27.0	41.0
38	35.793895243336635	38.0	35.0	40.0	27.0	41.0
39	35.63512709936736	38.0	35.0	40.0	26.0	41.0
40	35.5066252430129	38.0	35.0	40.0	26.0	41.0
41	35.37725953010975	38.0	34.0	40.0	26.0	41.0
42	35.18850921702564	38.0	34.0	40.0	25.0	41.0
43	31.759845323673588	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	12.0
11	13.0
12	16.0
13	11.0
14	25.0
15	46.0
16	102.0
17	194.0
18	406.0
19	908.0
20	1837.0
21	3598.0
22	6399.0
23	10744.0
24	16596.0
25	23834.0
26	32856.0
27	43887.0
28	56959.0
29	73607.0
30	93276.0
31	117481.0
32	146740.0
33	185462.0
34	239998.0
35	318393.0
36	442766.0
37	696216.0
38	1270019.0
39	912822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49379795298371	18.693250843836204	12.484516180697662	26.32843502248242
2	18.98865741016829	20.934975626295998	34.095242314317694	25.981124649218014
3	19.01662199716137	21.786777372070002	30.29039722066508	28.906203410103544
4	15.212138973561217	15.679145446521828	32.73539239022462	36.373323189692336
5	14.795204659032244	36.58110880332866	33.90396283542596	14.719723702213141
6	36.96096288483787	34.53952356692673	13.968577431023524	14.530936117211873
7	30.468940353005525	30.404577076620836	19.87351402190822	19.252968548465418
8	28.126943464252186	34.74549883030075	18.4535391708681	18.674018534578966
9	26.681133850056995	13.626676810307666	18.128783632048226	41.563405707587116
10	15.950059038716791	27.611121428924374	33.54698306193698	22.891836470421858
11	37.67364453751301	20.78418409856484	20.785249010483845	20.756922353438302
12	21.8065847337635	24.785910107803165	29.6773274288937	23.730177729539633
13	32.008590005503464	19.44999855171979	23.222086954743798	25.319324488032947
14	22.779296578821373	19.999067137158953	24.969884290930526	32.251751993089144
15	25.721563018079646	27.82795879387224	21.25951818273207	25.190960005316036
16	25.456463844962457	25.87420749254988	23.35145245466457	25.317876207823097
17	24.6216367951774	26.18350051030579	24.854383944195206	24.340478750321605
18	24.811723572719853	25.3615588947407	25.169065416261287	24.657652116278157
19	25.607532249792552	25.329185572402935	25.2309367987555	23.83234537904901
20	25.384624886906355	25.205762280990214	24.971758535907977	24.437854296195454
21	25.082956638490515	25.20750873653738	24.905819189883164	24.803715435088932
22	25.607638740984456	24.900324244381096	24.589902419991038	24.902134594643407
23	25.16934229336023	24.884265372642496	24.69013192980782	25.25626040418945
24	25.1826536923478	24.865246045769062	24.675840811854773	25.27625945002837
25	25.366713068428687	24.621274725124938	24.805121118822022	25.20689108762436
26	24.99182147646204	24.92445514846576	24.996996948388407	25.086726426683796
27	25.112710277507528	25.10566056060371	24.84920847226884	24.93242068961992
28	25.223461117084085	25.11505308372934	24.835492406752053	24.825993392434526
29	25.002577086843992	25.349674477724598	24.939342617093455	24.70840581833795
30	25.096246739239703	25.194431618171997	24.81498220319201	24.894339439396287
31	25.15089801892306	24.748233524108755	24.853787593520565	25.247080863447625
32	24.716712131306195	25.086982005544357	24.907672136622235	25.28863372652721
33	24.86247727477965	24.683210002334288	24.927223919455173	25.527088803430892
34	25.066322714315653	24.475104915122262	25.274725976865003	25.183846393697085
35	24.860965099854663	24.51544377861418	25.365626858271302	25.257964263259858
36	24.71971518291779	24.189793713782347	25.68365215376306	25.40683894953681
37	24.497681047805173	23.791261077213782	26.051004169343145	25.660053705637896
38	24.112417213747417	23.839757166005285	26.513644503435827	25.534181116811467
39	23.96373421161589	23.620875170172926	26.978691538465473	25.43669907974572
40	24.11680465085372	23.611269664663496	27.146777235761277	25.125148448721507
41	23.654249509714553	23.499965070889058	27.478987158014185	25.366798261382208
42	23.351069086373727	23.699380476842	27.346916781819143	25.60263365496513
43	23.5161943285347	23.014407832299373	27.335224048948465	26.134173790217464
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	721.0
1	668.0
2	615.0
3	1223.0
4	1831.0
5	1831.0
6	2083.0
7	2335.0
8	2446.0
9	2557.0
10	3829.0
11	5101.0
12	5101.0
13	10052.0
14	15003.0
15	23219.0
16	31435.0
17	24144.0
18	16853.0
19	16853.0
20	20803.5
21	24754.0
22	22958.5
23	21163.0
24	26044.5
25	30926.0
26	30926.0
27	38019.5
28	45113.0
29	53106.5
30	61100.0
31	72853.5
32	84607.0
33	84607.0
34	99537.0
35	114467.0
36	133536.5
37	152606.0
38	176035.0
39	199464.0
40	199464.0
41	225924.0
42	252384.0
43	281305.0
44	310226.0
45	336131.0
46	362036.0
47	362036.0
48	387717.5
49	413399.0
50	424045.0
51	434691.0
52	431479.0
53	428267.0
54	428267.0
55	413322.5
56	398378.0
57	372644.5
58	346911.0
59	319768.5
60	292626.0
61	292626.0
62	255847.5
63	219069.0
64	184761.0
65	150453.0
66	125063.0
67	99673.0
68	99673.0
69	82410.5
70	65148.0
71	53597.0
72	42046.0
73	34305.5
74	26565.0
75	26565.0
76	21817.5
77	17070.0
78	13893.0
79	10716.0
80	8591.0
81	6466.0
82	6466.0
83	5132.5
84	3799.0
85	2983.0
86	2167.0
87	1749.5
88	1332.0
89	1332.0
90	993.5
91	655.0
92	470.5
93	286.0
94	218.0
95	150.0
96	150.0
97	96.0
98	42.0
99	30.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4695224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.29024634328351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27499834674848	47.595244271853936
2	10.355792732435335	12.27995004372152
3	3.6561751577095207	6.5032657732437285
4	1.6700631244126691	3.960738162210436
5	0.9667292739371508	2.8658808399498636
6	0.6210578680397146	2.2093604393705486
7	0.43222112378853655	1.7938547832935217
8	0.33033454736159185	1.566849335101267
9	0.24645712784303306	1.3151253440584394
>10	1.3578100699735416	14.526331363705994
>50	0.07077647813641187	2.815618353066676
>100	0.0171523526070492	1.567493936639518
>500	2.158985276746546E-4	0.10573358417721368
>1k	7.196615976489352E-5	0.07518262542561199
>5k	1.0794923964734027E-4	0.41286440613973974
>10k+	3.598307988244676E-5	0.4065067380418872
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19054	0.4058166340945608	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7493	0.15958770018214252	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6844	0.145765143473453	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5015	0.10681066547623713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.259647676021421E-5
2	0.0	0.0	0.0	0.0	4.259647676021421E-5
3	0.0	0.0	0.0	0.0	4.259647676021421E-5
4	0.0	0.0	0.0	0.0	4.259647676021421E-5
5	0.0	0.0	0.0	0.0	4.259647676021421E-5
6	0.0	0.0	0.0	0.0	4.259647676021421E-5
7	0.0	0.0	0.0	0.0	4.259647676021421E-5
8	0.0	0.0	0.0	0.0	6.389471514032132E-5
9	0.0	0.0	0.0	0.0	8.519295352042842E-5
10	0.0	0.0	0.0	0.0	8.519295352042842E-5
11	0.0	0.0	0.0	0.0	8.519295352042842E-5
12	0.0	0.0	0.0	0.0	1.0649119190053552E-4
13	0.0	0.0	0.0	0.0	1.2778943028064264E-4
14	0.0	0.0	0.0	2.1298238380107105E-5	2.1298238380107104E-4
15	2.1298238380107105E-5	0.0	0.0	8.519295352042842E-5	2.1298238380107104E-4
16	2.1298238380107105E-5	0.0	0.0	1.0649119190053552E-4	2.768770989413924E-4
17	2.1298238380107105E-5	0.0	0.0	2.555788605612853E-4	2.768770989413924E-4
18	2.1298238380107105E-5	0.0	0.0	2.768770989413924E-4	3.194735757016066E-4
19	4.259647676021421E-5	2.1298238380107105E-5	0.0	3.407718140817137E-4	3.194735757016066E-4
20	4.259647676021421E-5	2.1298238380107105E-5	0.0	4.472630059822492E-4	3.620700524618208E-4
21	4.259647676021421E-5	2.1298238380107105E-5	0.0	6.815436281634274E-4	3.620700524618208E-4
22	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.0010862101573854623	3.620700524618208E-4
23	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.0013630872563268547	3.620700524618208E-4
24	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.0019381396925897466	3.833682908419279E-4
25	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.002236315029911246	3.833682908419279E-4
26	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.003066946326735423	4.04666529222035E-4
27	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.008306312968241771	4.04666529222035E-4
28	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.033502128971908476	4.04666529222035E-4
29	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.08517165528204831	4.259647676021421E-4
30	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.15752177105927215	4.259647676021421E-4
31	6.389471514032132E-5	2.1298238380107105E-5	0.0	0.43030960823168396	4.685612443623563E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2475	0.0	17.41616	37
AACGCAG	7805	0.0	15.951954	5
TCTTATA	4275	0.0	15.189473	37
TATACCG	170	2.4421752E-8	14.1470585	5
ACGCAGA	8990	0.0	13.890433	6
TTACGCG	80	0.0063024396	13.875	3
CTCTTAT	7060	0.0	13.80949	37
GTATTAA	705	0.0	13.6453905	1
CGCAGAG	9310	0.0	13.432868	7
AGAGTAC	9475	0.0	13.159894	10
GAGTACT	6035	0.0	12.936206	11
GTATTAG	460	0.0	12.869565	1
GTATAAG	620	0.0	12.830646	1
CAGAGTA	9955	0.0	12.729783	9
GGTATCA	5280	0.0	12.578598	1
CTAGTAC	325	0.0	12.523077	3
GTATAAA	665	0.0	12.518797	1
ATACCGT	300	1.8189894E-12	12.333333	6
CAACGCA	10070	0.0	12.308839	4
GCAGAGT	10440	0.0	12.209291	8
>>END_MODULE