FastQCFastQC Report
Thu 9 Feb 2017
SRR2727303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5558643
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176950.3183330895688031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC33350.020.19190437
TCTTATA56550.015.11405937
TACTTAC7000.014.831
GAGTACT48400.014.60123911
AGTACTT51100.013.75733912
GGGTAAG26000.013.5903841
GTACTTT52400.013.52194713
GGGTATA7550.013.2317871
CATTACG5800.013.07758624
CGCGTAA850.00941066613.05882410
AACGCAG136000.012.9908095
CTCTTAT88150.012.96993737
TACTTTT55150.012.78059814
TTACGCT1451.3741057E-512.758624
TACACCG3050.012.7377055
TCGTTAC7100.012.50704323
TCTATAC4500.012.3333333
ACTTTTT58900.012.24957515
TAATCGA6350.012.2362220
TTAGGCA13250.012.147174
Produced by FastQC (version 0.11.5)