##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727303.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5558643
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14006134230962	33.0	31.0	34.0	30.0	34.0
2	32.28420083822616	34.0	31.0	34.0	30.0	34.0
3	32.36011630896246	34.0	31.0	34.0	30.0	34.0
4	35.94277524208696	37.0	35.0	37.0	35.0	37.0
5	35.82259968844914	37.0	35.0	37.0	35.0	37.0
6	35.833971888462706	37.0	35.0	37.0	35.0	37.0
7	35.77637869530387	37.0	35.0	37.0	35.0	37.0
8	35.73892243124806	37.0	35.0	37.0	35.0	37.0
9	37.47793823060772	39.0	37.0	39.0	35.0	39.0
10	37.3067554437297	39.0	37.0	39.0	34.0	39.0
11	37.41265107329253	39.0	37.0	39.0	35.0	39.0
12	37.310954490151644	39.0	37.0	39.0	34.0	39.0
13	37.36418798616857	39.0	37.0	39.0	34.0	39.0
14	38.527309992744634	40.0	38.0	41.0	34.0	41.0
15	38.48591499759923	40.0	38.0	41.0	34.0	41.0
16	38.40319211001678	40.0	38.0	41.0	34.0	41.0
17	38.35603005985454	40.0	38.0	41.0	34.0	41.0
18	38.294224147872065	40.0	38.0	41.0	33.0	41.0
19	38.291212981297775	40.0	38.0	41.0	34.0	41.0
20	38.16431960102493	40.0	38.0	41.0	33.0	41.0
21	38.077569471541885	40.0	38.0	41.0	33.0	41.0
22	37.994561262523966	40.0	37.0	41.0	33.0	41.0
23	37.86732157470807	40.0	37.0	41.0	33.0	41.0
24	37.7412325274352	40.0	37.0	41.0	32.0	41.0
25	37.644012036750695	40.0	37.0	41.0	32.0	41.0
26	37.25572284458635	39.0	37.0	41.0	31.0	41.0
27	37.02046758534412	39.0	36.0	41.0	31.0	41.0
28	36.83325390747346	39.0	36.0	40.0	30.0	41.0
29	36.77746115373842	39.0	36.0	40.0	30.0	41.0
30	36.688564097388515	39.0	36.0	40.0	30.0	41.0
31	36.467416597899884	39.0	36.0	40.0	30.0	41.0
32	36.34235424005463	39.0	35.0	40.0	30.0	41.0
33	36.14750254693457	39.0	35.0	40.0	29.0	41.0
34	35.98061199469007	39.0	35.0	40.0	27.0	41.0
35	35.81438419412795	38.0	35.0	40.0	27.0	41.0
36	35.64863096982483	38.0	35.0	40.0	26.0	41.0
37	35.395903640510824	38.0	34.0	40.0	25.0	41.0
38	35.26895539073116	38.0	34.0	40.0	25.0	41.0
39	35.09294732545335	38.0	34.0	40.0	24.0	41.0
40	34.952068157642074	38.0	34.0	40.0	24.0	41.0
41	34.79835222373518	38.0	33.0	40.0	24.0	41.0
42	34.59662816986088	38.0	33.0	40.0	23.0	41.0
43	31.090716924976114	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	11.0
12	8.0
13	28.0
14	59.0
15	109.0
16	216.0
17	409.0
18	844.0
19	1749.0
20	3482.0
21	6589.0
22	11001.0
23	17364.0
24	25601.0
25	35970.0
26	47590.0
27	61285.0
28	78278.0
29	96956.0
30	120920.0
31	149335.0
32	185024.0
33	229448.0
34	293142.0
35	381885.0
36	530847.0
37	817412.0
38	1452968.0
39	1010104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25248086628337	17.586648396020397	12.151778770466102	27.00909196723013
2	19.596491445843885	20.518856850493908	33.518990156410474	26.36566154725173
3	19.09475028347746	21.244519570693782	30.05713804610226	29.6035920997265
4	15.365620709946654	15.049878180699858	32.23290288654983	37.35159822280366
5	15.14954279308817	36.452889671094184	33.50796228503971	14.889605250777931
6	37.88545153916162	33.9560032907312	13.36660764146933	14.791937528637837
7	31.42678887634986	30.16964752008719	19.098474213940346	19.305089389622612
8	29.176365526622234	34.10830664966252	17.958465762237292	18.756862061477957
9	27.907908458953024	13.07808398560584	16.858161245469443	42.15584630997169
10	16.22025375617754	26.996624895680476	32.55325085636908	24.2298704917729
11	38.783188630750345	20.292182822318324	19.712437010255922	21.21219153667541
12	22.685033019749604	24.301020950616902	28.377411537312252	24.63653449232124
13	33.18477189486715	18.621145484608384	22.34536378752872	25.848718832995747
14	23.36762767459612	19.32691126233507	24.099604885580888	33.205856177487924
15	27.293351992563654	26.79810522100448	20.2992349031949	25.60930788323697
16	26.29276605819082	25.060450905014047	22.627734862627445	26.019048174167686
17	25.89772359908704	25.091321748851293	23.546034526772093	25.46492012528957
18	25.801135277081116	24.42729277631249	24.160896823199476	25.610675123406917
19	27.128743472102812	24.37598528993497	23.76696974423434	24.728301493727876
20	26.336553723633628	24.10984119685326	23.765368634035323	25.788236445477793
21	26.121051486846703	24.516505197401596	23.848536414373076	25.51390690137863
22	26.81382848295888	24.047523829107213	23.00413967941456	26.134508008519347
23	26.09014466300498	24.140514150665908	23.462434986380668	26.306906199948443
24	25.88694758774758	23.937928735484544	23.556576668082478	26.618547008685393
25	26.25390765336072	23.564977999126764	23.56487005911335	26.616244288399166
26	25.881712497096864	23.897739070488967	23.961747498445213	26.258800933968956
27	26.64729503225877	24.209955559297473	23.477474628250096	25.665274780193652
28	26.08917320288423	24.51278126693871	23.419582801054144	25.97846272912292
29	25.92668750268726	24.536114299839006	23.68119341357234	25.856004783901394
30	26.35207909556343	24.373106889577187	23.372790805237898	25.902023209621483
31	25.993394430978928	23.72755364933492	23.708969977024967	26.570081942661183
32	25.5740474788541	24.288949659116444	23.69556742535903	26.441435436670425
33	25.898641089201085	23.72512499903304	23.7626701336999	26.61356377806598
34	26.092879143344877	23.727139879283488	24.204144788575196	25.975836188796436
35	25.96191192706565	23.87656483785701	24.002926613563776	26.158596621513556
36	25.58789978057594	23.23725412839069	24.65670128482797	26.518144806205402
37	25.361045852378002	23.186342422062364	24.76939065883526	26.68322106672438
38	25.211243103757518	22.9654971546113	25.24465053791006	26.578609203721125
39	24.840145337630066	22.796283193578002	25.838806341763625	26.524765127028303
40	25.219320614761553	22.47517964366483	26.132565088277843	26.172934653295776
41	24.51110819673075	22.50425148727846	26.582782884239915	26.40185743175088
42	24.091239534541074	22.557861693942208	26.63729259101547	26.713606180501248
43	24.139830530580937	21.668561913402247	26.551930030404904	27.639677525611916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	194.0
1	222.5
2	251.0
3	730.0
4	1209.0
5	1209.0
6	1441.5
7	1674.0
8	1736.5
9	1799.0
10	2537.0
11	3275.0
12	3275.0
13	6938.0
14	10601.0
15	18606.0
16	26611.0
17	19625.5
18	12640.0
19	12640.0
20	15870.5
21	19101.0
22	16964.0
23	14827.0
24	19544.0
25	24261.0
26	24261.0
27	30585.0
28	36909.0
29	44985.5
30	53062.0
31	64927.0
32	76792.0
33	76792.0
34	91597.0
35	106402.0
36	126399.5
37	146397.0
38	173870.0
39	201343.0
40	201343.0
41	231088.0
42	260833.0
43	294214.0
44	327595.0
45	365589.5
46	403584.0
47	403584.0
48	443709.0
49	483834.0
50	507958.0
51	532082.0
52	536060.5
53	540039.0
54	540039.0
55	513799.5
56	487560.0
57	462382.5
58	437205.0
59	407233.0
60	377261.0
61	377261.0
62	335912.5
63	294564.0
64	252452.5
65	210341.0
66	179169.0
67	147997.0
68	147997.0
69	124512.5
70	101028.0
71	86412.0
72	71796.0
73	60300.5
74	48805.0
75	48805.0
76	41329.0
77	33853.0
78	28927.5
79	24002.0
80	19440.0
81	14878.0
82	14878.0
83	12226.5
84	9575.0
85	7777.0
86	5979.0
87	5196.5
88	4414.0
89	4414.0
90	3335.5
91	2257.0
92	1660.5
93	1064.0
94	774.0
95	484.0
96	484.0
97	336.5
98	189.0
99	132.5
100	76.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5558643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.56658483878775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.42122487452177	46.622872093814394
2	9.252154789240375	10.467255976543244
3	3.016480030189547	5.118959206266781
4	1.434649718172149	3.2461293998771117
5	0.8272444148423198	2.3397195687295707
6	0.5416697806274863	1.8384245760283353
7	0.4018880202957314	1.5913402957025664
8	0.2868352744844234	1.2980233511104087
9	0.22323509714760364	1.1364882355615453
>10	1.4292983955131577	15.27443904847375
>50	0.1163744301168512	4.480744642091524
>100	0.045892513542562685	4.54035502648222
>500	0.002162304891483987	0.8250625407850055
>1k	7.949610845101802E-4	0.7086274128144368
>5k	6.359688675319414E-5	0.19327346812064397
>10k+	3.179844337659707E-5	0.3182851575983988
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17695	0.3183330895688031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.799000223615728E-5	0.0	0.0	0.0	1.799000223615728E-5
2	5.397000670847183E-5	0.0	0.0	0.0	1.799000223615728E-5
3	5.397000670847183E-5	0.0	0.0	0.0	1.799000223615728E-5
4	5.397000670847183E-5	0.0	0.0	0.0	1.799000223615728E-5
5	7.196000894462912E-5	0.0	0.0	0.0	1.799000223615728E-5
6	7.196000894462912E-5	0.0	0.0	0.0	1.799000223615728E-5
7	7.196000894462912E-5	0.0	0.0	0.0	1.799000223615728E-5
8	7.196000894462912E-5	0.0	0.0	0.0	3.598000447231456E-5
9	1.2593001565310095E-4	0.0	0.0	0.0	1.0794001341694366E-4
10	1.9789002459773007E-4	0.0	0.0	0.0	1.2593001565310095E-4
11	2.518600313062019E-4	0.0	0.0	1.799000223615728E-5	1.2593001565310095E-4
12	2.518600313062019E-4	0.0	0.0	3.598000447231456E-5	1.2593001565310095E-4
13	2.698500335423592E-4	0.0	0.0	5.397000670847183E-5	1.2593001565310095E-4
14	2.8784003577851647E-4	0.0	0.0	5.397000670847183E-5	1.9789002459773007E-4
15	2.8784003577851647E-4	0.0	0.0	1.4392001788925824E-4	1.9789002459773007E-4
16	2.8784003577851647E-4	0.0	0.0	4.137700514316174E-4	2.518600313062019E-4
17	2.8784003577851647E-4	0.0	0.0	5.397000670847184E-4	2.518600313062019E-4
18	3.058300380146737E-4	0.0	0.0	7.016100872101338E-4	2.8784003577851647E-4
19	3.058300380146737E-4	0.0	0.0	0.0010614101319332794	2.8784003577851647E-4
20	3.418100424869883E-4	0.0	0.0	0.0015111601878372114	3.058300380146737E-4
21	3.7779004695930285E-4	0.0	0.0	0.0027524703421320635	3.058300380146737E-4
22	3.7779004695930285E-4	0.0	0.0	0.005001220621651723	3.058300380146737E-4
23	3.7779004695930285E-4	0.0	0.0	0.005990670744640374	3.7779004695930285E-4
24	3.9578004919546013E-4	0.0	0.0	0.007843640974964573	3.7779004695930285E-4
25	3.9578004919546013E-4	0.0	0.0	0.009156911138204054	3.9578004919546013E-4
26	4.137700514316174E-4	0.0	0.0	0.01043420129697122	3.9578004919546013E-4
27	4.137700514316174E-4	0.0	0.0	0.014967681860482855	4.137700514316174E-4
28	4.3176005366777465E-4	0.0	0.0	0.03990182495979684	4.137700514316174E-4
29	4.3176005366777465E-4	0.0	0.0	0.10331658284225125	4.137700514316174E-4
30	4.677400581400892E-4	0.0	0.0	0.19747625454629844	4.137700514316174E-4
31	4.677400581400892E-4	0.0	0.0	0.49105510103814903	4.137700514316174E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3335	0.0	20.191904	37
TCTTATA	5655	0.0	15.114059	37
TACTTAC	700	0.0	14.8	31
GAGTACT	4840	0.0	14.601239	11
AGTACTT	5110	0.0	13.757339	12
GGGTAAG	2600	0.0	13.590384	1
GTACTTT	5240	0.0	13.521947	13
GGGTATA	755	0.0	13.231787	1
CATTACG	580	0.0	13.077586	24
CGCGTAA	85	0.009410666	13.058824	10
AACGCAG	13600	0.0	12.990809	5
CTCTTAT	8815	0.0	12.969937	37
TACTTTT	5515	0.0	12.780598	14
TTACGCT	145	1.3741057E-5	12.75862	4
TACACCG	305	0.0	12.737705	5
TCGTTAC	710	0.0	12.507043	23
TCTATAC	450	0.0	12.333333	3
ACTTTTT	5890	0.0	12.249575	15
TAATCGA	635	0.0	12.23622	20
TTAGGCA	1325	0.0	12.14717	4
>>END_MODULE