FastQCFastQC Report
Thu 9 Feb 2017
SRR2727302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170149
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT317340.3460576267626622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92410.10077262648622176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC52450.019.08198237
TCTTATA91800.015.77941237
GAGTACT91450.015.05084811
AGTACTT95550.014.34693912
GTACTTT97550.014.01486313
GTATAGA7800.013.993591
TATACCG4400.013.8755
TACTTTT101150.013.60751314
TATACTA4500.013.1555565
CTCTTAT145150.013.03858137
TAAGACT12350.012.8825914
ACTTTTT108450.012.77685515
TAATACT9900.012.7070714
GTCTTAG13600.012.6507351
AACGCAG233450.012.1484255
TATACTG9850.012.0203045
TTAGACA15950.011.830724
CTAAGAC14100.011.8085113
GGACCGT8000.011.7937516
CTTAGAC16050.011.7570093
Produced by FastQC (version 0.11.5)