##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727302.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9170149
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06508127621481	33.0	31.0	34.0	30.0	34.0
2	32.21302227477438	33.0	31.0	34.0	30.0	34.0
3	32.29565070316742	34.0	31.0	34.0	30.0	34.0
4	35.890940921461585	37.0	35.0	37.0	35.0	37.0
5	35.76382826494967	37.0	35.0	37.0	35.0	37.0
6	35.77779946650812	37.0	35.0	37.0	35.0	37.0
7	35.71996518268133	37.0	35.0	37.0	33.0	37.0
8	35.683429026071444	37.0	35.0	37.0	33.0	37.0
9	37.40711094225404	39.0	37.0	39.0	35.0	39.0
10	37.23182524078943	39.0	37.0	39.0	34.0	39.0
11	37.34342408176792	39.0	37.0	39.0	34.0	39.0
12	37.23979414074951	39.0	37.0	39.0	34.0	39.0
13	37.29633586106398	39.0	37.0	39.0	34.0	39.0
14	38.44425046964886	40.0	38.0	41.0	34.0	41.0
15	38.40093863251295	40.0	38.0	41.0	34.0	41.0
16	38.319836242573594	40.0	38.0	41.0	34.0	41.0
17	38.27032406997967	40.0	38.0	41.0	33.0	41.0
18	38.21513009221551	40.0	38.0	41.0	33.0	41.0
19	38.21318159606785	40.0	38.0	41.0	34.0	41.0
20	38.08867991130788	40.0	38.0	41.0	33.0	41.0
21	38.0049591342518	40.0	37.0	41.0	33.0	41.0
22	37.923233853670204	40.0	37.0	41.0	33.0	41.0
23	37.8022769313781	40.0	37.0	41.0	33.0	41.0
24	37.67980945565879	40.0	37.0	41.0	32.0	41.0
25	37.58682568843756	39.0	37.0	41.0	32.0	41.0
26	37.202590710358145	39.0	37.0	41.0	31.0	41.0
27	36.972138402549405	39.0	36.0	40.0	31.0	41.0
28	36.80347854762229	39.0	36.0	40.0	30.0	41.0
29	36.74557229113726	39.0	36.0	40.0	30.0	41.0
30	36.66232042685457	39.0	36.0	40.0	30.0	41.0
31	36.44337425705951	39.0	36.0	40.0	30.0	41.0
32	36.32802051526098	39.0	35.0	40.0	30.0	41.0
33	36.14069705955705	39.0	35.0	40.0	29.0	41.0
34	35.98463536415821	38.0	35.0	40.0	28.0	41.0
35	35.81804755844207	38.0	35.0	40.0	27.0	41.0
36	35.65552937035156	38.0	35.0	40.0	26.0	41.0
37	35.409314396091055	38.0	34.0	40.0	25.0	41.0
38	35.289906412643894	38.0	34.0	40.0	25.0	41.0
39	35.11918148767266	38.0	34.0	40.0	24.0	41.0
40	34.97383597583856	38.0	34.0	40.0	24.0	41.0
41	34.83260948104551	38.0	33.0	40.0	24.0	41.0
42	34.63069040644814	38.0	33.0	40.0	23.0	41.0
43	31.102343157128637	35.0	27.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	6.0
10	29.0
11	41.0
12	40.0
13	41.0
14	73.0
15	143.0
16	328.0
17	646.0
18	1315.0
19	2607.0
20	5086.0
21	9778.0
22	17399.0
23	27411.0
24	40901.0
25	57504.0
26	77737.0
27	101125.0
28	129817.0
29	163657.0
30	203832.0
31	252080.0
32	312595.0
33	388291.0
34	495859.0
35	645456.0
36	897758.0
37	1386551.0
38	2412854.0
39	1539188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09565744242542	17.862806809355007	12.181067068812077	26.8604686794075
2	19.087072630989965	20.719052656614412	33.833855916626874	26.360018795768745
3	18.941077184242044	21.44771039161959	30.08188852765642	29.529323896481944
4	15.079242441971227	15.112993256707169	32.66726636611902	37.14049793520258
5	15.01180624218865	36.612218623710476	33.64588732418633	14.730087809914538
6	37.70964899261724	34.39164401799796	13.412213912772847	14.48649307661195
7	31.434963597647105	30.368961289505762	19.20629642986172	18.98977868298541
8	29.097836905376344	34.4944994895939	18.03152816819007	18.376135436839686
9	27.725394647349788	13.23745121262479	16.950706035419927	42.086448104605495
10	15.949849887935299	27.208881774985333	32.868320896421636	23.972947440657723
11	38.615043223398004	20.366288486697435	20.070415431635844	20.948252858268717
12	22.548673963749117	24.313781597223773	28.786086245708763	24.351458193318344
13	33.000499773776845	18.539229842394057	22.67588018471674	25.784390199112362
14	23.28494335261074	19.169481324676404	24.44320152267973	33.10237380003313
15	27.017096450668358	26.899181245582817	20.485196042070854	25.59852626167797
16	26.222649163061583	25.069799847308914	22.830185202007076	25.877365787622423
17	25.64227691393019	25.407242564979043	23.880135426370934	25.070345094719837
18	25.609420304948156	24.478795273664584	24.470812851568716	25.44097156981855
19	26.577081790055974	24.829345739093224	24.083087417663553	24.51048505318725
20	26.07988158098631	24.466003769404402	23.997407239511592	25.4567074100977
21	25.870506575193055	24.880860714476942	23.828838549951588	25.419794160378416
22	26.51750805793886	24.364925804368063	23.221629223254713	25.895936914438362
23	25.9671462263045	24.224099303075665	23.563521159797947	26.245233310821884
24	25.775295472298215	24.09978289338592	23.693540857405914	26.43138077690995
25	26.107798248425407	23.78774870506466	23.747596685724517	26.356856360785414
26	25.745775777471007	24.06036150557641	24.12368653988065	26.07017617707193
27	26.30882006388337	24.468620956976817	23.73826205004957	25.484296929090245
28	26.12805964221519	24.589862171268972	23.61248437729856	25.66959380921728
29	25.7250236610114	24.809695022403673	24.006163912930965	25.459117403653963
30	25.980581122509566	24.684582551493982	23.691567061778386	25.64326926421806
31	25.83197939313745	24.08148439027545	23.78422640679012	26.30230980979698
32	25.334757374171346	24.525403022350016	23.8833741959918	26.256465407486836
33	25.73111952706548	24.064559910640494	23.883112477234558	26.321208085059467
34	25.854247297399418	24.057090021110888	24.39729169068027	25.69137099080942
35	25.714478576084204	24.07678435759332	24.337870627838218	25.870866438484256
36	25.418736380401235	23.64094629214858	24.70932587900153	26.230991448448655
37	25.041806845232284	23.35460416182987	24.99382507307133	26.609763919866513
38	24.902692420810173	23.248466300820194	25.449521049221772	26.39932022914786
39	24.671572948269436	23.02273387269934	25.92615452595154	26.37953865307968
40	24.946879270991126	22.787383280249863	26.406364825696944	25.859372623062065
41	24.39143573348699	22.719260068729525	26.691278407799047	26.198025789984435
42	23.960428560103004	22.741833311541612	26.972527927299762	26.325210201055622
43	23.963361991173755	21.780496696400462	26.93091464489836	27.32522666752743
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	213.0
1	293.5
2	374.0
3	1217.0
4	2060.0
5	2060.0
6	2454.5
7	2849.0
8	2847.5
9	2846.0
10	4091.5
11	5337.0
12	5337.0
13	11757.5
14	18178.0
15	32530.5
16	46883.0
17	34603.0
18	22323.0
19	22323.0
20	26967.0
21	31611.0
22	27883.5
23	24156.0
24	31398.0
25	38640.0
26	38640.0
27	50108.0
28	61576.0
29	75327.0
30	89078.0
31	109085.5
32	129093.0
33	129093.0
34	154666.0
35	180239.0
36	215993.0
37	251747.0
38	299502.5
39	347258.0
40	347258.0
41	398712.0
42	450166.0
43	505583.5
44	561001.0
45	623144.5
46	685288.0
47	685288.0
48	749650.0
49	814012.0
50	851259.5
51	888507.0
52	900003.5
53	911500.0
54	911500.0
55	862772.0
56	814044.0
57	761722.5
58	709401.0
59	658392.5
60	607384.0
61	607384.0
62	536210.5
63	465037.0
64	398294.5
65	331552.0
66	280206.0
67	228860.0
68	228860.0
69	190931.0
70	153002.0
71	128997.5
72	104993.0
73	86772.0
74	68551.0
75	68551.0
76	57114.5
77	45678.0
78	38377.0
79	31076.0
80	24923.5
81	18771.0
82	18771.0
83	15122.5
84	11474.0
85	9162.5
86	6851.0
87	5728.5
88	4606.0
89	4606.0
90	3404.0
91	2202.0
92	1613.5
93	1025.0
94	769.0
95	513.0
96	513.0
97	327.0
98	141.0
99	97.0
100	53.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9170149.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.049375569797874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59053342484377	43.55868008449729
2	9.940996394168831	10.74609295292875
3	3.501277716014848	5.677256227411518
4	1.6509044520754073	3.569214190403002
5	0.9737741972162209	2.631594365275897
6	0.6340189275788015	2.056099628104027
7	0.43041046741987615	1.6284391901924395
8	0.32657893652102166	1.4121110074567902
9	0.23270901530964522	1.1319999270253969
>10	1.5316664100836095	15.619888721302603
>50	0.1292614703521038	4.8097980929522315
>100	0.05526532794283703	5.082678010677482
>500	0.0017964694519449857	0.6383858966577257
>1k	6.656027064448271E-4	0.6197470767656735
>5k	1.2101855550978415E-4	0.472062335902521
>10k+	2.0169759251630692E-5	0.3459522924467831
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31734	0.3460576267626622	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9241	0.10077262648622176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1809896436797264E-5
2	0.0	1.0904948218398632E-5	0.0	0.0	2.1809896436797264E-5
3	0.0	1.0904948218398632E-5	0.0	0.0	2.1809896436797264E-5
4	0.0	1.0904948218398632E-5	0.0	0.0	2.1809896436797264E-5
5	0.0	1.0904948218398632E-5	0.0	0.0	2.1809896436797264E-5
6	1.0904948218398632E-5	1.0904948218398632E-5	0.0	0.0	5.452474109199316E-5
7	1.0904948218398632E-5	1.0904948218398632E-5	0.0	0.0	6.542968931039179E-5
8	1.0904948218398632E-5	1.0904948218398632E-5	0.0	0.0	8.723958574718906E-5
9	1.0904948218398632E-5	1.0904948218398632E-5	0.0	0.0	1.7447917149437811E-4
10	3.2714844655195896E-5	1.0904948218398632E-5	0.0	0.0	1.8538411971277675E-4
11	5.452474109199316E-5	1.0904948218398632E-5	0.0	0.0	2.07194016149574E-4
12	5.452474109199316E-5	1.0904948218398632E-5	0.0	1.0904948218398632E-5	2.2900391258637127E-4
13	5.452474109199316E-5	1.0904948218398632E-5	0.0	2.1809896436797264E-5	2.2900391258637127E-4
14	6.542968931039179E-5	1.0904948218398632E-5	0.0	7.633463752879042E-5	3.2714844655195896E-4
15	6.542968931039179E-5	1.0904948218398632E-5	0.0	1.1995443040238495E-4	3.4895834298875623E-4
16	8.723958574718906E-5	1.0904948218398632E-5	0.0	2.5081380902316857E-4	4.252929805175467E-4
17	8.723958574718906E-5	1.0904948218398632E-5	0.0	4.14388032299148E-4	4.252929805175467E-4
18	8.723958574718906E-5	1.0904948218398632E-5	0.0	5.234375144831343E-4	4.689127733911412E-4
19	8.723958574718906E-5	1.0904948218398632E-5	0.0	8.396810128166946E-4	4.689127733911412E-4
20	8.723958574718906E-5	2.1809896436797264E-5	0.0	0.0012976888379894374	4.907226698279384E-4
21	1.1995443040238495E-4	2.1809896436797264E-5	0.0	0.0023881836598293005	5.125325662647357E-4
22	1.3085937862078359E-4	2.1809896436797264E-5	0.0	0.0037294922906923322	5.125325662647357E-4
23	1.4176432683918222E-4	2.1809896436797264E-5	0.0	0.004765462371440202	6.542968931039179E-4
24	1.4176432683918222E-4	2.1809896436797264E-5	0.0	0.006477539241728787	6.542968931039179E-4
25	1.4176432683918222E-4	2.1809896436797264E-5	0.0	0.00765527364931584	6.652018413223165E-4
26	1.5266927505758085E-4	2.1809896436797264E-5	0.0	0.009269205985638837	6.761067895407152E-4
27	1.5266927505758085E-4	2.1809896436797264E-5	0.0	0.013871094133803061	6.761067895407152E-4
28	1.5266927505758085E-4	2.1809896436797264E-5	0.0	0.03816731876439521	6.761067895407152E-4
29	1.5266927505758085E-4	2.1809896436797264E-5	0.0	0.0948403346554129	6.870117377591138E-4
30	1.5266927505758085E-4	2.1809896436797264E-5	0.0	0.1745991259247805	6.870117377591138E-4
31	1.5266927505758085E-4	2.1809896436797264E-5	0.0	0.4782474090660904	6.870117377591138E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5245	0.0	19.081982	37
TCTTATA	9180	0.0	15.779412	37
GAGTACT	9145	0.0	15.050848	11
AGTACTT	9555	0.0	14.346939	12
GTACTTT	9755	0.0	14.014863	13
GTATAGA	780	0.0	13.99359	1
TATACCG	440	0.0	13.875	5
TACTTTT	10115	0.0	13.607513	14
TATACTA	450	0.0	13.155556	5
CTCTTAT	14515	0.0	13.038581	37
TAAGACT	1235	0.0	12.882591	4
ACTTTTT	10845	0.0	12.776855	15
TAATACT	990	0.0	12.707071	4
GTCTTAG	1360	0.0	12.650735	1
AACGCAG	23345	0.0	12.148425	5
TATACTG	985	0.0	12.020304	5
TTAGACA	1595	0.0	11.83072	4
CTAAGAC	1410	0.0	11.808511	3
GGACCGT	800	0.0	11.793751	6
CTTAGAC	1605	0.0	11.757009	3
>>END_MODULE