##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	809145
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05726167744965	33.0	31.0	34.0	30.0	34.0
2	32.19916331436269	33.0	31.0	34.0	30.0	34.0
3	32.27575156492347	34.0	31.0	34.0	30.0	34.0
4	35.875757744285636	37.0	35.0	37.0	35.0	37.0
5	35.74634707005543	37.0	35.0	37.0	35.0	37.0
6	35.761234389386324	37.0	35.0	37.0	35.0	37.0
7	35.695928418268664	37.0	35.0	37.0	33.0	37.0
8	35.65236144325183	37.0	35.0	37.0	33.0	37.0
9	37.3880379907186	39.0	37.0	39.0	34.0	39.0
10	37.2129655376972	39.0	37.0	39.0	34.0	39.0
11	37.32186196540793	39.0	37.0	39.0	34.0	39.0
12	37.20659832292111	39.0	37.0	39.0	34.0	39.0
13	37.25149880429342	39.0	37.0	39.0	34.0	39.0
14	38.38049422538606	40.0	38.0	41.0	34.0	41.0
15	38.32965043348226	40.0	38.0	41.0	34.0	41.0
16	38.21556457742432	40.0	38.0	41.0	33.0	41.0
17	38.14919946363136	40.0	38.0	41.0	33.0	41.0
18	38.08047754110821	40.0	37.0	41.0	33.0	41.0
19	38.059541862089	40.0	37.0	41.0	33.0	41.0
20	37.91438617305921	40.0	37.0	41.0	33.0	41.0
21	37.7976135303314	40.0	37.0	41.0	32.0	41.0
22	37.70687824802724	40.0	37.0	41.0	32.0	41.0
23	37.557773946573235	39.0	37.0	41.0	32.0	41.0
24	37.4056800697032	39.0	37.0	41.0	31.0	41.0
25	37.27834936877815	39.0	37.0	41.0	31.0	41.0
26	36.82402536010233	39.0	36.0	40.0	30.0	41.0
27	36.54175580396591	39.0	36.0	40.0	30.0	41.0
28	36.32087450333377	39.0	35.0	40.0	29.0	41.0
29	36.23087827274469	39.0	35.0	40.0	29.0	41.0
30	36.12337467326623	39.0	35.0	40.0	28.0	41.0
31	35.88761470441021	39.0	35.0	40.0	27.0	41.0
32	35.72069530183094	38.0	35.0	40.0	26.0	41.0
33	35.49952480704942	38.0	34.0	40.0	25.0	41.0
34	35.286722404513405	38.0	34.0	40.0	25.0	41.0
35	35.09774144312824	38.0	34.0	40.0	24.0	41.0
36	34.88817084700517	38.0	33.0	40.0	24.0	41.0
37	34.61339438543153	38.0	33.0	40.0	23.0	41.0
38	34.46120781812901	38.0	33.0	40.0	22.0	41.0
39	34.25957523064469	38.0	33.0	40.0	22.0	41.0
40	34.089374586755156	38.0	33.0	40.0	21.0	41.0
41	33.8940301182112	38.0	32.0	40.0	20.0	41.0
42	33.659351537734274	38.0	32.0	40.0	19.0	41.0
43	29.891534891768472	34.0	24.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	2.0
14	11.0
15	17.0
16	40.0
17	89.0
18	176.0
19	374.0
20	710.0
21	1321.0
22	2225.0
23	3506.0
24	4968.0
25	6964.0
26	9029.0
27	11232.0
28	13970.0
29	17193.0
30	20795.0
31	24911.0
32	30481.0
33	36709.0
34	45702.0
35	59054.0
36	80033.0
37	118793.0
38	198845.0
39	121990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46335329267313	15.81595387724079	11.243596635955237	28.47709619413084
2	20.773903317699546	19.37613159569669	31.05945164340137	28.790513443202393
3	20.266330509364824	20.061175685445747	28.178385827014935	31.49410797817449
4	16.711467042371886	14.005771524263267	29.18253217902848	40.10022925433637
5	16.735690142063536	35.09037317168122	31.868577325448467	16.30535936080678
6	41.00130384541708	31.340241860235185	11.873520815181458	15.78493347916628
7	33.12793133492761	28.73292178781306	17.519604026472386	20.619542850786942
8	30.962559244634768	32.53965605670183	16.976438092060135	19.521346606603267
9	28.579673606090378	12.491951380778477	15.023141711312556	43.90523330181858
10	17.339043063974938	25.605299420993767	30.776931205161002	26.278726309870294
11	40.89922078243084	18.776980640058333	17.543950713407362	22.779847864103466
12	24.365225021473282	22.99822652305829	25.944175642190213	26.692372813278215
13	35.78258532154311	16.73556655482021	20.403883111185266	27.077965012451415
14	24.961533470515175	17.81411242731525	22.05698607789704	35.167368024272534
15	30.072607505453284	24.44753412552756	18.5280759320023	26.951782437016853
16	28.390461536560196	22.869695790000556	21.020089106402438	27.719753567036808
17	27.970759258229368	23.261220176853346	21.509000240995125	27.25902032392217
18	27.708754302380907	22.584703606893697	22.423422254354904	27.28311983637049
19	29.15151178095397	23.152339815484247	21.38430071248046	26.311847691081326
20	28.278615081351305	22.267084391549105	21.44251030408641	28.011790223013183
21	27.79007470848859	23.31732878532278	21.31138423891886	27.581212267269773
22	28.867137534063737	22.496338727916505	20.229625098097376	28.40689863992239
23	28.26415537388231	22.08207428829196	20.87870530003893	28.775065037786803
24	27.894382341854673	22.08195070104864	21.134654480964475	28.889012476132216
25	28.584864270310018	21.407287939738858	20.842123476014805	29.165724313936316
26	28.05158531536375	21.695493391172164	21.77026367338363	28.482657620080452
27	29.470243281488486	22.050806715730804	21.197436800573442	27.281513202207268
28	28.449041889896126	22.859808810534577	20.742882919625035	27.948266379944265
29	28.074448955378827	22.538852739620214	21.565726785681182	27.820971519319777
30	28.337071847443905	22.534032837130553	21.305822812969243	27.8230725024563
31	28.101514561666946	21.73244597692626	21.240197986763807	28.925841474642986
32	27.525227246043666	22.129284615242014	21.32522601017123	29.020262128543088
33	28.36562050065192	21.976160020762656	21.151585933299966	28.506633545285453
34	28.425807488151072	22.053402047840624	21.634070531239765	27.88671993276854
35	28.133523657688052	22.237299865907843	21.70550395788147	27.923672518522636
36	27.69763145048168	21.538908353879712	22.107286085930212	28.65617410970839
37	26.91347039158618	21.40370390968244	22.55430114503581	29.128524553695566
38	27.53437270204969	21.021819327808984	22.68320263982352	28.760605330317805
39	26.622669608043058	20.857942643160374	23.449814310166907	29.069573438629664
40	27.44168226955614	20.331831748326938	24.12719599082983	28.0992899912871
41	27.020373357062084	20.0963980497933	24.363989149040037	28.519239444104578
42	25.91698644865877	20.109498297585723	25.36850626278356	28.605008990971953
43	26.196417205816015	18.77253149929864	25.056695647875227	29.974355647010114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	5.0
2	9.0
3	43.0
4	77.0
5	77.0
6	99.0
7	121.0
8	110.5
9	100.0
10	137.5
11	175.0
12	175.0
13	368.5
14	562.0
15	1016.0
16	1470.0
17	1054.0
18	638.0
19	638.0
20	795.5
21	953.0
22	799.0
23	645.0
24	891.5
25	1138.0
26	1138.0
27	1467.5
28	1797.0
29	2226.5
30	2656.0
31	3232.0
32	3808.0
33	3808.0
34	4698.0
35	5588.0
36	6958.0
37	8328.0
38	10736.5
39	13145.0
40	13145.0
41	15985.0
42	18825.0
43	23018.0
44	27211.0
45	33422.5
46	39634.0
47	39634.0
48	49102.0
49	58570.0
50	67637.5
51	76705.0
52	85992.0
53	95279.0
54	95279.0
55	91987.0
56	88695.0
57	84712.0
58	80729.0
59	77335.0
60	73941.0
61	73941.0
62	67701.0
63	61461.0
64	53442.5
65	45424.0
66	39062.0
67	32700.0
68	32700.0
69	27632.0
70	22564.0
71	19270.0
72	15976.0
73	13337.5
74	10699.0
75	10699.0
76	8948.0
77	7197.0
78	6079.0
79	4961.0
80	4000.5
81	3040.0
82	3040.0
83	2406.0
84	1772.0
85	1452.5
86	1133.0
87	923.5
88	714.0
89	714.0
90	566.0
91	418.0
92	297.0
93	176.0
94	127.0
95	78.0
96	78.0
97	52.5
98	27.0
99	16.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	809145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.14459617996019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94869116200317	60.12032138772783
2	7.141004397951656	9.87523730831374
3	2.235723940069648	4.63764687017956
4	1.1212414634455719	3.1011115284068658
5	0.6588765394412546	2.277887612605758
6	0.4137550204326974	1.716535428315002
7	0.3028036683688872	1.4656066159824106
8	0.21454996292503709	1.1867976437501697
9	0.15610673736273356	0.9714543584325583
>10	0.7481483996402325	8.76138541975502
>50	0.035888942174997746	1.6808676927622546
>100	0.020710252927655038	2.512176043755969
>500	0.0014282932894413743	0.6293402154648765
>1k	0.0010712199670810307	1.0636318745479358
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2058	0.2543425467623232	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	1416	0.17499953654783754	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	1397	0.1726513789246674	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	1350	0.16684277848840443	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1319	0.16301157394533736	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.13297987381742454	No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC	854	0.10554350579933139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.2358724332474401E-4	0.0	0.0	0.0	0.0
11	1.2358724332474401E-4	0.0	0.0	0.0	0.0
12	1.2358724332474401E-4	0.0	0.0	0.0	0.0
13	1.2358724332474401E-4	0.0	0.0	0.0	1.2358724332474401E-4
14	1.2358724332474401E-4	0.0	0.0	0.0	1.2358724332474401E-4
15	1.2358724332474401E-4	0.0	0.0	0.0	1.2358724332474401E-4
16	1.2358724332474401E-4	0.0	0.0	0.0	2.4717448664948803E-4
17	1.2358724332474401E-4	0.0	0.0	1.2358724332474401E-4	2.4717448664948803E-4
18	1.2358724332474401E-4	0.0	0.0	2.4717448664948803E-4	2.4717448664948803E-4
19	2.4717448664948803E-4	0.0	0.0	2.4717448664948803E-4	2.4717448664948803E-4
20	2.4717448664948803E-4	0.0	0.0	7.415234599484641E-4	2.4717448664948803E-4
21	2.4717448664948803E-4	0.0	0.0	0.0019773958931959042	2.4717448664948803E-4
22	2.4717448664948803E-4	0.0	0.0	0.0035840300564175765	2.4717448664948803E-4
23	2.4717448664948803E-4	0.0	0.0	0.004696315246340272	2.4717448664948803E-4
24	3.7076172997423204E-4	0.0	0.0	0.0066737111395361775	2.4717448664948803E-4
25	3.7076172997423204E-4	0.0	0.0	0.009269043249355801	2.4717448664948803E-4
26	3.7076172997423204E-4	0.0	0.0	0.012111549845824915	2.4717448664948803E-4
27	3.7076172997423204E-4	0.0	0.0	0.016437103362190956	2.4717448664948803E-4
28	3.7076172997423204E-4	0.0	0.0	0.038559219917320135	2.4717448664948803E-4
29	3.7076172997423204E-4	0.0	0.0	0.10121795228296535	2.4717448664948803E-4
30	3.7076172997423204E-4	0.0	0.0	0.18451575428384281	2.4717448664948803E-4
31	3.7076172997423204E-4	0.0	0.0	0.40548974534848514	2.4717448664948803E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATGA	25	0.005495394	29.599998	2
TAGATCG	55	6.2579056E-7	26.90909	5
CGCAATC	70	1.9214531E-7	23.785715	22
CTATTCT	40	0.0019306954	23.125002	4
TCTATAC	40	0.0019306954	23.125002	3
TTAGATC	65	2.6812158E-6	22.76923	4
TTAGGGT	75	3.7379323E-7	22.2	4
CCCTTAA	60	3.7245314E-5	21.583334	1
GTCTTAG	80	6.9548696E-7	20.8125	1
TCTAAAC	45	0.0038248443	20.555555	3
TGTTACG	90	9.469659E-8	20.555555	24
TCTTAGA	170	0.0	18.5	2
TTCGTCG	60	9.2335936E-4	18.5	18
GCGCAAT	80	1.6157062E-5	18.5	21
ACTAAAC	70	1.2186311E-4	18.5	3
TAAGACT	60	9.2335936E-4	18.5	4
GTAAGAC	50	0.007033127	18.499998	3
TACACCG	50	0.007033127	18.499998	5
CTAAACC	75	2.066313E-4	17.266666	4
AAGAGTA	65	0.0015793873	17.076923	5
>>END_MODULE