##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727300.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565969
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.376119195207565	33.0	31.0	34.0	31.0	34.0
2	32.50934916336147	34.0	31.0	34.0	31.0	34.0
3	32.60050486312309	34.0	31.0	34.0	31.0	34.0
4	36.117073198767024	37.0	35.0	37.0	35.0	37.0
5	35.93242075673273	37.0	35.0	37.0	35.0	37.0
6	35.95729481234942	37.0	35.0	37.0	35.0	37.0
7	35.91956226464253	37.0	35.0	37.0	35.0	37.0
8	35.88087375931452	37.0	35.0	37.0	35.0	37.0
9	37.72343769257246	39.0	37.0	39.0	35.0	39.0
10	37.55320443763574	39.0	37.0	39.0	35.0	39.0
11	37.66446079073085	39.0	37.0	39.0	35.0	39.0
12	37.597985656165605	39.0	37.0	39.0	35.0	39.0
13	37.67767752746063	39.0	37.0	39.0	35.0	39.0
14	38.88639047133117	40.0	38.0	41.0	36.0	41.0
15	38.81284239981762	40.0	38.0	41.0	35.0	41.0
16	38.77838769477557	40.0	38.0	41.0	35.0	41.0
17	38.738975037181454	40.0	38.0	41.0	35.0	41.0
18	38.72202706439272	40.0	38.0	41.0	35.0	41.0
19	38.777712713342346	40.0	38.0	41.0	35.0	41.0
20	38.743272057109685	40.0	38.0	41.0	35.0	41.0
21	38.7008618944564	40.0	38.0	41.0	35.0	41.0
22	38.65291075366115	40.0	38.0	41.0	34.0	41.0
23	38.580489779810456	40.0	38.0	41.0	34.0	41.0
24	38.53977569160054	40.0	38.0	41.0	34.0	41.0
25	38.42208370663787	40.0	38.0	41.0	34.0	41.0
26	38.30548944455478	40.0	38.0	41.0	34.0	41.0
27	38.200597840697995	40.0	38.0	41.0	34.0	41.0
28	38.0903523632971	40.0	38.0	41.0	33.0	41.0
29	37.98044661165068	40.0	37.0	41.0	33.0	41.0
30	37.89496343797355	40.0	37.0	41.0	33.0	41.0
31	37.76518692260191	40.0	37.0	41.0	33.0	41.0
32	37.64727079527117	40.0	37.0	41.0	33.0	41.0
33	37.53172189232354	40.0	36.0	41.0	33.0	41.0
34	37.40446777682062	40.0	36.0	41.0	33.0	41.0
35	37.31298065287372	40.0	36.0	41.0	32.0	41.0
36	37.18241293410023	39.0	36.0	41.0	32.0	41.0
37	37.08152524092112	39.0	36.0	41.0	31.0	41.0
38	36.95632033584317	39.0	36.0	41.0	31.0	41.0
39	36.80719669418743	39.0	35.0	41.0	31.0	41.0
40	36.65763051503574	39.0	35.0	41.0	30.0	41.0
41	36.50807136028874	39.0	35.0	41.0	30.0	41.0
42	36.35932831365116	39.0	35.0	41.0	30.0	41.0
43	34.69331512948213	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	10.0
11	9.0
12	5.0
13	1.0
14	4.0
15	3.0
16	12.0
17	15.0
18	38.0
19	82.0
20	178.0
21	325.0
22	666.0
23	1154.0
24	2112.0
25	3494.0
26	5299.0
27	8195.0
28	12016.0
29	16932.0
30	22171.0
31	28392.0
32	36620.0
33	48701.0
34	65406.0
35	90711.0
36	134544.0
37	228770.0
38	419040.0
39	441059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77259766955795	17.219625675859483	12.86602736069488	25.141749293887685
2	16.28582685864152	21.673481403527145	37.20354617492428	24.837145562907057
3	18.633638341499736	23.297268336729527	31.232482890785196	26.83661043098554
4	13.257669851702044	14.90891582144985	35.11895829355498	36.714456033293125
5	14.222248333140694	37.992003673125076	34.38963351126363	13.396114482470598
6	34.81339668920649	36.06099482173658	14.744417035075408	14.381191453981529
7	28.88250022829315	31.382549718417156	21.545190230457948	18.189759822831743
8	27.96983848339271	36.54114481193433	19.083583391497534	16.405433313175422
9	27.074865466685484	13.679389566460129	18.652859667081533	40.59288529977286
10	14.779602916788262	28.374955059774493	34.243589751776696	22.60185227166055
11	36.404041203880794	21.659496452356336	21.55655699442326	20.379905349339612
12	20.67416404794731	25.72617976473353	31.66410063034453	21.935555556974627
13	31.924642186403435	19.10044196277193	25.047111405142758	23.927804445681875
14	21.322133452194773	19.79106866100159	28.450180048264045	30.436617838539586
15	25.23089537532352	28.466400037293205	23.10441649866632	23.198288088716954
16	23.236539165206974	26.47587532064811	25.68556593393611	24.602019580208804
17	23.228556887141444	26.925437221298758	27.040637458340488	22.805368433219304
18	23.03710993001777	26.150900816044253	27.83758810040301	22.974401153534966
19	24.305525843742757	26.41987165774035	27.648823188709354	21.625779309807537
20	23.0893459576786	26.290367178405194	27.550417664717504	23.069869199198706
21	22.556576790472864	26.953917989436572	28.032994267447187	22.45651095264338
22	23.421919591000844	25.998088084757743	27.027674238762074	23.552318085479342
23	22.57203048080773	26.114437769840908	27.720472116625555	23.593059632725808
24	22.6293751664305	25.887230206983663	27.993274451793106	23.490120174792732
25	23.05818314411077	26.009135557600437	27.422509640995447	23.510171657293345
26	22.622670052855455	26.290367178405194	28.189574633980623	22.897388134758735
27	23.565792170853957	26.76445063727315	27.518680127128953	22.151077064743937
28	22.896047112043725	27.219440487008363	27.065350591231375	22.81916180971654
29	22.555618917105	27.14932415648075	27.831074561501534	22.463982364912717
30	23.084875881961903	26.88686685368612	27.47781086343344	22.550446400918535
31	22.61283588627872	26.284683796422538	27.935865907945818	23.16661440935293
32	22.185113498415358	26.94574413669747	27.630687452944468	23.238454911942704
33	22.63224878653409	26.16514120011316	27.649334054505548	23.553275958847205
34	22.86360713398541	26.473576424565238	28.256370336832976	22.406446104616375
35	22.71992612880587	26.562530931327505	27.751379497295286	22.966163442571343
36	22.459001423399823	26.183340794102566	28.279550872335275	23.078106910162333
37	21.950115232166155	26.10932911187897	28.312693290863354	23.627862365091516
38	22.4444417482083	25.890039968862727	28.40592629866875	23.259591984260226
39	21.7655649632911	25.905302084524024	29.06628419847392	23.26284875371096
40	22.528926179253865	25.644696670240595	28.910406272410246	22.915970878095287
41	21.86754654785631	25.64546296893489	29.076948521969463	23.410041961239337
42	21.413706146162536	25.815070413271275	29.049681060097615	23.72154238046858
43	20.96829503010596	25.27910833483932	28.963280882316315	24.7893157527384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	306.5
2	406.0
3	1984.5
4	3563.0
5	3563.0
6	4613.5
7	5664.0
8	5168.5
9	4673.0
10	6081.0
11	7489.0
12	7489.0
13	11594.0
14	15699.0
15	24738.5
16	33778.0
17	26663.5
18	19549.0
19	19549.0
20	20568.5
21	21588.0
22	17101.5
23	12615.0
24	14943.5
25	17272.0
26	17272.0
27	20315.0
28	23358.0
29	26270.5
30	29183.0
31	32570.0
32	35957.0
33	35957.0
34	40552.5
35	45148.0
36	51285.5
37	57423.0
38	64306.0
39	71189.0
40	71189.0
41	78414.0
42	85639.0
43	93162.0
44	100685.0
45	109896.5
46	119108.0
47	119108.0
48	127752.0
49	136396.0
50	138354.0
51	140312.0
52	138023.5
53	135735.0
54	135735.0
55	124852.5
56	113970.0
57	103255.0
58	92540.0
59	84372.0
60	76204.0
61	76204.0
62	66273.0
63	56342.0
64	47309.5
65	38277.0
66	31759.5
67	25242.0
68	25242.0
69	20544.5
70	15847.0
71	12994.0
72	10141.0
73	8220.0
74	6299.0
75	6299.0
76	4992.0
77	3685.0
78	2980.5
79	2276.0
80	1763.0
81	1250.0
82	1250.0
83	957.0
84	664.0
85	500.5
86	337.0
87	256.5
88	176.0
89	176.0
90	113.0
91	50.0
92	36.5
93	23.0
94	16.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1565969.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.85860124685036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.83221114311895	51.85743320743962
2	8.710446161657758	10.776320315922913
3	2.8063383854184036	5.207885014419808
4	1.3581944991554857	3.360640477556994
5	0.800692653318404	2.476486378145288
6	0.5317764006272268	1.9736966591330982
7	0.3698922410307882	1.6016711649559174
8	0.2606610832983835	1.28993040098614
9	0.1917486935412352	1.0675175376034602
>10	1.0562095374084377	11.35749346295202
>50	0.05298069831188733	2.245070406090083
>100	0.026367053374947932	3.009971205925311
>500	0.0013441186081362025	0.5728071384723974
>1k	8.271499126992015E-4	1.0977737522041344
>5k	2.0678747817480037E-4	0.8878638562163711
>10k+	1.0339373908740019E-4	1.2174390219765612
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19035	1.2155413038189133	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7136	0.45569229020497853	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6746	0.4307875826405248	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4953	0.316289786068562	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2504	0.1599009942086976	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2276	0.14534131901717084	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1903	0.1215222012696292	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	1672	0.10677095140452972	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1585	0.10121528587092082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	6.385822452424026E-5
9	6.385822452424026E-5	0.0	0.0	6.385822452424026E-5	6.385822452424026E-5
10	6.385822452424026E-5	0.0	0.0	6.385822452424026E-5	6.385822452424026E-5
11	6.385822452424026E-5	0.0	0.0	1.2771644904848052E-4	6.385822452424026E-5
12	6.385822452424026E-5	0.0	0.0	1.9157467357272079E-4	6.385822452424026E-5
13	6.385822452424026E-5	0.0	0.0	2.5543289809696105E-4	6.385822452424026E-5
14	6.385822452424026E-5	0.0	0.0	3.192911226212013E-4	1.2771644904848052E-4
15	6.385822452424026E-5	0.0	0.0	7.662986942908831E-4	2.5543289809696105E-4
16	6.385822452424026E-5	0.0	0.0	0.0011494480414363248	3.192911226212013E-4
17	6.385822452424026E-5	0.0	0.0	0.0015325973885817663	3.192911226212013E-4
18	6.385822452424026E-5	0.0	0.0	0.001724172062154487	3.8314934714544157E-4
19	6.385822452424026E-5	0.0	0.0	0.0025543289809696105	3.8314934714544157E-4
20	1.2771644904848052E-4	0.0	0.0	0.004086926369551377	5.108657961939221E-4
21	1.2771644904848052E-4	0.0	0.0	0.00727983759576339	5.108657961939221E-4
22	1.9157467357272079E-4	0.0	0.0	0.013346368925566215	5.108657961939221E-4
23	1.9157467357272079E-4	0.0	0.0	0.016666996600826708	6.385822452424026E-4
24	1.9157467357272079E-4	0.0	0.0	0.02413840887016282	6.385822452424026E-4
25	1.9157467357272079E-4	0.0	0.0	0.028353051688762676	6.385822452424026E-4
26	1.9157467357272079E-4	0.0	0.0	0.03493044881475942	7.024404697666429E-4
27	1.9157467357272079E-4	0.0	0.0	0.05191673653820733	7.024404697666429E-4
28	1.9157467357272079E-4	0.0	0.0	0.1517909996941191	7.024404697666429E-4
29	1.9157467357272079E-4	0.0	0.0	0.3403004784896764	7.024404697666429E-4
30	1.9157467357272079E-4	0.0	0.0	0.5682743400412141	7.024404697666429E-4
31	1.9157467357272079E-4	0.0	0.0	1.1076209043729475	7.024404697666429E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	35	2.3888571E-5	31.714287	5
CGCTACG	70	1.2195553E-4	18.5	10
TGTTACG	155	2.1827873E-11	17.903227	24
TATACAC	780	0.0	16.602564	37
TACACCG	90	4.4476146E-5	16.444443	5
CTTATAC	2000	0.0	15.910001	37
TAGACTG	215	0.0	15.488372	5
GTACTTA	610	0.0	15.163935	30
GTATTAC	125	2.960218E-6	14.800001	1
GAGTGTC	650	0.0	14.8	7
GTATACG	75	0.004105289	14.8	1
CGATAGC	75	0.004105289	14.8	4
TCTATAC	200	6.184564E-11	14.799999	3
TACTTAC	565	0.0	14.734512	31
GTATTAT	165	1.6460035E-8	14.575757	1
AGTGTCT	775	0.0	14.56129	8
GTTACGG	445	0.0	14.550561	25
TACTCCG	140	5.998336E-7	14.535715	5
CGTTACG	295	0.0	14.423729	24
CTAGGAC	270	0.0	14.388889	3
>>END_MODULE