FastQCFastQC Report
Thu 9 Feb 2017
SRR2727299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9103701
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT399720.4390741743385465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120150.13197929062037517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118870.13057326904739072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92370.1014642286691973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC48100.019.46153837
TCTTATA81750.014.98103937
GAGTACT113050.014.12251211
AGTACTT116050.013.82119812
GTACTTT117700.013.43882713
GTCTTAG12750.013.2039211
TACTTTT121650.013.00246514
CTCTTAT129750.012.96069237
TATACCG3000.012.955
AACGCAG231350.012.9144165
ACTTTTT130550.012.28609815
TAATACT10700.012.1028044
TCTATAC6950.011.9784173
AGAGTAC254100.011.77272710
ACGCAGA255950.011.702096
GTATAAA11700.011.7008541
TTAGACA12850.011.3735414
CAGAGTA265900.011.3546469
CGCAGAG264050.011.3220987
CTAGACA11450.011.3100434
Produced by FastQC (version 0.11.5)