##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727299.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9103701
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08376439428316	33.0	31.0	34.0	30.0	34.0
2	32.2343530394946	33.0	31.0	34.0	30.0	34.0
3	32.31563273002925	34.0	31.0	34.0	30.0	34.0
4	35.909552170046005	37.0	35.0	37.0	35.0	37.0
5	35.778847196321585	37.0	35.0	37.0	35.0	37.0
6	35.79260742416738	37.0	35.0	37.0	35.0	37.0
7	35.73490737448429	37.0	35.0	37.0	35.0	37.0
8	35.70134498046454	37.0	35.0	37.0	33.0	37.0
9	37.43271357440232	39.0	37.0	39.0	35.0	39.0
10	37.26093904006733	39.0	37.0	39.0	34.0	39.0
11	37.37317789764844	39.0	37.0	39.0	34.0	39.0
12	37.267065119998996	39.0	37.0	39.0	34.0	39.0
13	37.32816137085346	39.0	37.0	39.0	34.0	39.0
14	38.481027441476826	40.0	38.0	41.0	34.0	41.0
15	38.43780216419674	40.0	38.0	41.0	34.0	41.0
16	38.367168912950895	40.0	38.0	41.0	34.0	41.0
17	38.31577739646766	40.0	38.0	41.0	34.0	41.0
18	38.266724489303854	40.0	38.0	41.0	33.0	41.0
19	38.265159191849555	40.0	38.0	41.0	34.0	41.0
20	38.145814433053104	40.0	38.0	41.0	33.0	41.0
21	38.06566461266687	40.0	38.0	41.0	33.0	41.0
22	37.98802003712556	40.0	37.0	41.0	33.0	41.0
23	37.8647143617744	40.0	37.0	41.0	33.0	41.0
24	37.751247761761945	40.0	37.0	41.0	32.0	41.0
25	37.655827009257	40.0	37.0	41.0	32.0	41.0
26	37.28099648703313	39.0	37.0	41.0	31.0	41.0
27	37.060391812077306	39.0	36.0	40.0	31.0	41.0
28	36.899131243436045	39.0	36.0	40.0	30.0	41.0
29	36.85442667767757	39.0	36.0	40.0	30.0	41.0
30	36.76183345652499	39.0	36.0	40.0	30.0	41.0
31	36.5568145307057	39.0	36.0	40.0	30.0	41.0
32	36.43919698153531	39.0	35.0	40.0	30.0	41.0
33	36.25111303633544	39.0	35.0	40.0	29.0	41.0
34	36.10847039022921	39.0	35.0	40.0	29.0	41.0
35	35.943667745678376	38.0	35.0	40.0	27.0	41.0
36	35.791242374941795	38.0	35.0	40.0	27.0	41.0
37	35.544147264942026	38.0	35.0	40.0	26.0	41.0
38	35.42997765414308	38.0	34.0	40.0	26.0	41.0
39	35.26211537483491	38.0	34.0	40.0	25.0	41.0
40	35.122806537692746	38.0	34.0	40.0	24.0	41.0
41	34.98163724841139	38.0	34.0	40.0	24.0	41.0
42	34.77682054803865	38.0	33.0	40.0	24.0	41.0
43	31.27506944703039	35.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	20.0
11	34.0
12	28.0
13	35.0
14	66.0
15	119.0
16	246.0
17	514.0
18	1116.0
19	2216.0
20	4567.0
21	8826.0
22	15224.0
23	25090.0
24	37808.0
25	53653.0
26	72954.0
27	95590.0
28	123922.0
29	156467.0
30	196051.0
31	243441.0
32	303452.0
33	378091.0
34	483876.0
35	635577.0
36	888259.0
37	1378432.0
38	2421409.0
39	1576611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90514374318752	17.871160311613927	12.304764842342692	26.918931102855858
2	19.017133800857476	20.63798009183298	33.68538795375639	26.65949815355315
3	19.02784373080794	21.416235001566942	29.985189539946443	29.570731727678666
4	15.18584584445381	15.112293340917063	32.38226958464475	37.319591229984376
5	15.018386478202656	36.37213041157656	33.6757984472469	14.933684662973883
6	37.72637084631844	34.212733919973864	13.511043475615029	14.549851758092668
7	31.128361970587566	30.077064262106145	19.467807653173143	19.326766114133143
8	29.02285564958691	34.17370583678001	18.194995639685445	18.608442873947638
9	27.27272128115807	13.267219562681158	17.22331390277427	42.23674525338651
10	15.967308240901145	27.19023834372416	33.142696580215016	23.699756835159675
11	38.48137147738046	20.22218216525345	20.140918512152364	21.15552784521372
12	22.372175887586817	24.419420189656933	29.041485435428953	24.166918487327298
13	32.89437998897372	18.678425400834232	22.701800070103356	25.725394540088697
14	23.12987871635942	19.200828322459184	24.71108178970289	32.9582111714785
15	26.642581956503186	27.03652064144022	20.73717051998962	25.583726882066976
16	26.0950354147176	25.05489800247174	22.933826583276407	25.91623999953426
17	25.43525979159465	25.343143409477094	24.316165480390886	24.90543131853737
18	25.441048646039672	24.505791655503625	24.677842560954055	25.375317137502652
19	26.25283936719802	24.684070797140638	24.568249770066043	24.494840065595298
20	26.035993493195793	24.38294052056411	24.29418540876947	25.286880577470633
21	25.650633736762664	24.708961772799874	24.257431126088168	25.382973364349294
22	26.29042847518828	24.26376920771014	23.81402904159528	25.631773275506305
23	25.747264766274725	24.093431891051782	24.00945505569658	26.149848286976912
24	25.75326232704699	24.17611255026939	24.02427320493061	26.046351917753007
25	25.95366433937143	23.72265960843837	24.127956311394673	26.195719740795532
26	25.608859517684074	24.122518962342898	24.475935666164784	25.79268585380825
27	26.051064286931215	24.257431126088168	24.16198642727831	25.529518159702302
28	25.80625176507884	24.48201011874182	24.203222403723494	25.508515712455846
29	25.714827409204233	24.636892182640885	24.259067823075473	25.389212585079406
30	25.680665478798126	24.528441784280922	24.194412799805264	25.596479937115685
31	25.701854663284745	24.042452624487556	24.14530090564266	26.11039180658504
32	25.22357665305572	24.32171267487805	24.259441297555796	26.19526937451043
33	25.484569407540953	24.08607224688069	24.33634408687192	26.093014258706432
34	25.785534915964398	23.844917578026784	24.606915363323115	25.762632142685703
35	25.50781270166935	23.94230654104303	24.72463671642994	25.82524404085767
36	25.313408250117174	23.67762297992871	25.002369915268524	26.00659885468558
37	25.012750308912825	23.29461391581292	25.28819872269531	26.404437052578945
38	24.762335669855588	23.31421034148639	25.76068787847931	26.16276611017871
39	24.5483238080864	23.0413542799791	26.15010093147831	26.26022098045619
40	24.76119327732754	22.974930745199124	26.548686078332317	25.715189899141023
41	24.405261113035237	22.718134086345763	26.83919430130669	26.037410499312312
42	23.891843548025136	22.873883929184405	26.96998726122486	26.2642852615656
43	24.181209378471458	21.976424752965855	26.957618665200012	26.884747203362675
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	304.0
1	437.5
2	571.0
3	1670.0
4	2769.0
5	2769.0
6	3286.5
7	3804.0
8	3743.5
9	3683.0
10	5309.5
11	6936.0
12	6936.0
13	14732.0
14	22528.0
15	40045.0
16	57562.0
17	41289.0
18	25016.0
19	25016.0
20	29953.0
21	34890.0
22	30833.5
23	26777.0
24	34573.5
25	42370.0
26	42370.0
27	53949.5
28	65529.0
29	79152.0
30	92775.0
31	112669.5
32	132564.0
33	132564.0
34	158590.0
35	184616.0
36	220161.5
37	255707.0
38	301207.5
39	346708.0
40	346708.0
41	397223.0
42	447738.0
43	500838.5
44	553939.0
45	611880.5
46	669822.0
47	669822.0
48	728011.5
49	786201.0
50	822904.5
51	859608.0
52	868342.5
53	877077.0
54	877077.0
55	851739.0
56	826401.0
57	771597.0
58	716793.0
59	664224.5
60	611656.0
61	611656.0
62	540293.0
63	468930.0
64	400951.0
65	332972.0
66	280319.0
67	227666.0
68	227666.0
69	188280.5
70	148895.0
71	123601.0
72	98307.0
73	81267.0
74	64227.0
75	64227.0
76	52727.5
77	41228.0
78	34217.0
79	27206.0
80	21911.5
81	16617.0
82	16617.0
83	13305.5
84	9994.0
85	7966.5
86	5939.0
87	4899.0
88	3859.0
89	3859.0
90	2899.5
91	1940.0
92	1437.5
93	935.0
94	679.0
95	423.0
96	423.0
97	283.5
98	144.0
99	109.5
100	75.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9103701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.45809737421331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76176966459127	42.63912449469293
2	10.198111281450403	10.903432518336778
3	3.611790464277824	5.79238339003857
4	1.7734382760627725	3.7921854419568266
5	1.065363991395945	2.8476165995512486
6	0.6886035743488247	2.208686215786248
7	0.4861745136946177	1.819297514576337
8	0.350439017525811	1.498704249809476
9	0.26522505804386903	1.2760584281091476
>10	1.6179528592986578	16.190572443136283
>50	0.13012672743678977	4.763508010960242
>100	0.04917189541630843	4.398366024932103
>500	0.0011328108190730329	0.41175373002887833
>1k	5.969442882667366E-4	0.5801619944185065
>5k	4.1168539792773715E-5	0.17528206362750812
>10k+	6.175280968916057E-5	0.7028668800391396
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39972	0.4390741743385465	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12015	0.13197929062037517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11887	0.13057326904739072	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9237	0.1014642286691973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.787634831152736E-5
2	0.0	0.0	0.0	0.0	8.787634831152736E-5
3	0.0	0.0	0.0	0.0	8.787634831152736E-5
4	0.0	0.0	0.0	0.0	8.787634831152736E-5
5	0.0	0.0	0.0	0.0	9.886089185046829E-5
6	0.0	0.0	0.0	0.0	1.3181452246729104E-4
7	1.098454353894092E-5	0.0	0.0	0.0	1.647681530841138E-4
8	2.196908707788184E-5	1.098454353894092E-5	0.0	0.0	1.8673724016199565E-4
9	2.196908707788184E-5	1.098454353894092E-5	0.0	1.098454353894092E-5	2.636290449345821E-4
10	2.196908707788184E-5	1.098454353894092E-5	0.0	2.196908707788184E-5	2.74613588473523E-4
11	3.295363061682276E-5	1.098454353894092E-5	0.0	2.196908707788184E-5	2.74613588473523E-4
12	3.295363061682276E-5	1.098454353894092E-5	0.0	3.295363061682276E-5	2.855981320124639E-4
13	3.295363061682276E-5	1.098454353894092E-5	0.0	3.295363061682276E-5	2.9658267555140484E-4
14	3.295363061682276E-5	1.098454353894092E-5	0.0	7.689180477258644E-5	3.4052084970716853E-4
15	3.295363061682276E-5	3.295363061682276E-5	0.0	1.3181452246729104E-4	3.5150539324610945E-4
16	7.689180477258644E-5	3.295363061682276E-5	0.0	1.9772178370093657E-4	4.283971980186959E-4
17	7.689180477258644E-5	3.295363061682276E-5	0.0	3.185517626292867E-4	4.283971980186959E-4
18	7.689180477258644E-5	3.295363061682276E-5	0.0	3.844590238629322E-4	4.3938174155763683E-4
19	7.689180477258644E-5	3.295363061682276E-5	0.0	6.261189817196325E-4	4.5036628509657775E-4
20	8.787634831152736E-5	3.295363061682276E-5	0.0	9.66639831426801E-4	4.613508286355187E-4
21	1.3181452246729104E-4	3.295363061682276E-5	0.0	0.0016257124437632563	4.613508286355187E-4
22	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.002636290449345821	4.833199157134005E-4
23	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.0033722548664548626	5.49227176947046E-4
24	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.004448740133271073	5.49227176947046E-4
25	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.0050309209408349416	5.60211720485987E-4
26	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.006107406207651152	5.821808075638688E-4
27	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.009600491053034364	5.931653511028097E-4
28	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.03225061983033054	5.931653511028097E-4
29	1.4279906600623196E-4	3.295363061682276E-5	0.0	0.08527301149279837	6.041498946417507E-4
30	1.4279906600623196E-4	4.393817415576368E-5	0.0	0.16313145609681162	6.041498946417507E-4
31	1.4279906600623196E-4	4.393817415576368E-5	0.0	0.4167645664109575	6.041498946417507E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4810	0.0	19.461538	37
TCTTATA	8175	0.0	14.981039	37
GAGTACT	11305	0.0	14.122512	11
AGTACTT	11605	0.0	13.821198	12
GTACTTT	11770	0.0	13.438827	13
GTCTTAG	1275	0.0	13.203921	1
TACTTTT	12165	0.0	13.002465	14
CTCTTAT	12975	0.0	12.960692	37
TATACCG	300	0.0	12.95	5
AACGCAG	23135	0.0	12.914416	5
ACTTTTT	13055	0.0	12.286098	15
TAATACT	1070	0.0	12.102804	4
TCTATAC	695	0.0	11.978417	3
AGAGTAC	25410	0.0	11.772727	10
ACGCAGA	25595	0.0	11.70209	6
GTATAAA	1170	0.0	11.700854	1
TTAGACA	1285	0.0	11.373541	4
CAGAGTA	26590	0.0	11.354646	9
CGCAGAG	26405	0.0	11.322098	7
CTAGACA	1145	0.0	11.310043	4
>>END_MODULE