FastQCFastQC Report
Thu 9 Feb 2017
SRR2727298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719765
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88630.3258737427682171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27660.10169996304827808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT2300.016.8913044
TATACTG3100.016.7096795
CGTATAC803.3846905E-416.18753
ATTATAC1902.7284841E-1115.5789483
TTATACT3100.015.51612954
TCTTATA14100.015.48226937
CTTATAC8200.015.34146437
TAAGGGT3200.015.0312514
TATTAGT1508.113602E-814.82
TAGTACG1152.2119291E-514.4782612
CGCCTAA908.279138E-414.38888922
GTAAGAC2600.014.2307693
GAGTACT28000.014.07321511
ACCGTGC3050.013.950828
AGTACTT28350.013.76895912
GTACTTT28050.013.7183613
TATACCG950.001245838913.6315795
TTTATAC2051.4260877E-913.5365863
TAAGACT4000.013.41254
AACGTTA850.00940904813.05882412
Produced by FastQC (version 0.11.5)