##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727298.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2719765
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.929001218855305	31.0	30.0	31.0	26.0	34.0
2	30.18265438374271	31.0	30.0	31.0	27.0	34.0
3	30.201581386627154	31.0	30.0	31.0	27.0	34.0
4	33.93994848819659	35.0	33.0	37.0	30.0	37.0
5	32.34601151202401	35.0	32.0	35.0	25.0	37.0
6	32.40547363467064	35.0	32.0	35.0	25.0	37.0
7	32.48046945232401	35.0	32.0	35.0	26.0	37.0
8	32.337335394785946	35.0	32.0	35.0	25.0	37.0
9	33.60033973523448	35.0	32.0	37.0	27.0	39.0
10	33.004453693609555	35.0	32.0	37.0	25.0	39.0
11	33.302588275089946	35.0	32.0	37.0	25.0	39.0
12	33.13367625511763	35.0	32.0	37.0	25.0	39.0
13	33.33295523694143	35.0	32.0	37.0	26.0	39.0
14	33.83563947620475	36.0	32.0	38.0	25.0	40.0
15	33.80123062102792	36.0	32.0	38.0	25.0	40.0
16	33.676662505767965	36.0	32.0	38.0	25.0	40.0
17	33.53793802038044	36.0	31.0	38.0	25.0	40.0
18	33.40646930892927	36.0	31.0	38.0	24.0	40.0
19	33.37248401975906	36.0	31.0	38.0	24.0	40.0
20	33.17197257851322	36.0	31.0	38.0	24.0	40.0
21	33.01656135732315	36.0	31.0	38.0	23.0	40.0
22	32.86845260528023	36.0	30.0	38.0	23.0	40.0
23	32.66487619334759	35.0	30.0	38.0	21.0	40.0
24	32.44396960766831	35.0	30.0	38.0	19.0	40.0
25	32.26550749789044	35.0	30.0	38.0	19.0	40.0
26	31.733197904966055	34.0	29.0	38.0	18.0	40.0
27	31.36004176831454	34.0	27.0	38.0	16.0	40.0
28	31.10540616560622	34.0	27.0	38.0	16.0	39.0
29	30.949178697424227	34.0	27.0	38.0	16.0	39.0
30	30.782285601881046	34.0	27.0	38.0	16.0	39.0
31	30.475133329533985	34.0	26.0	38.0	15.0	39.0
32	30.248289466185497	34.0	26.0	38.0	15.0	39.0
33	30.003419780753116	33.0	25.0	37.0	14.0	39.0
34	29.777751754287596	33.0	25.0	37.0	12.0	39.0
35	29.533053774866577	33.0	25.0	37.0	11.0	39.0
36	29.27692649916445	33.0	24.0	37.0	9.0	39.0
37	28.90351776716003	32.0	24.0	37.0	9.0	39.0
38	28.669138326289218	32.0	24.0	37.0	9.0	39.0
39	28.397305649568988	31.0	23.0	37.0	9.0	39.0
40	28.157692300621562	31.0	23.0	37.0	9.0	39.0
41	27.89987039321412	31.0	22.0	36.0	9.0	39.0
42	27.580121811994786	31.0	21.0	36.0	9.0	38.0
43	24.919508119267658	28.0	15.0	34.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	12.0
10	47.0
11	101.0
12	222.0
13	505.0
14	959.0
15	1744.0
16	3102.0
17	5287.0
18	8938.0
19	14103.0
20	21108.0
21	30463.0
22	42133.0
23	56336.0
24	72684.0
25	91307.0
26	112276.0
27	133747.0
28	157707.0
29	181100.0
30	204606.0
31	225653.0
32	243306.0
33	252312.0
34	247148.0
35	222918.0
36	177555.0
37	122894.0
38	72721.0
39	16769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.653596910027154	21.773976795789345	13.804722099151947	25.767704195031556
2	21.399937126920893	23.39724204113223	30.88744799642616	24.315372835520716
3	21.417144495939908	23.18615762758915	29.04622274350909	26.350475132961854
4	18.349452985827817	16.873369574209537	31.062095438392657	33.71508200156999
5	15.257825584195695	37.51802085841975	32.58020453973046	14.643949017654098
6	36.993269639104845	35.63149757423895	12.924976974113573	14.450255812542629
7	30.89645612764338	30.74868600779847	19.05131509523801	19.303542769320142
8	29.201603815035494	34.62494002239164	17.71064779493817	18.462808367634704
9	27.686289072769156	13.218715587559954	16.775456703060744	42.31953863661015
10	16.071719431642073	28.11158316986945	32.714848525516	23.10184887297248
11	39.142646515415855	20.45720861912702	19.672104023693223	20.728040841763903
12	22.48282480287819	24.982856974775395	28.380246087437698	24.154072134908713
13	33.14183394521218	19.12838057699838	22.349688300275943	25.3800971775135
14	23.351539563160788	19.601252314078607	24.30849724148961	32.738710881271
15	27.02303324000419	27.552343676751484	20.147770119844914	25.27685296339941
16	26.144133776263757	25.597983649322643	22.568273361852953	25.689609212560644
17	25.725568201664483	25.65633427888071	23.703297895222565	24.914799624232238
18	25.72218555647271	24.96561283787386	24.11689980568174	25.195301799971688
19	26.76444472224622	24.858287388800136	24.1339968710532	24.243271017900444
20	26.280285245232584	24.524398247642722	23.931883820844817	25.26343268627988
21	26.031807895167415	24.771294578759562	23.920890223971554	25.276007302101466
22	26.597959750199006	24.458767577345835	23.412721319672837	25.530551352782314
23	26.07725299796122	24.46601085020213	23.643145639421054	25.813590512415598
24	26.033352146233224	24.39122497715795	23.575382431938053	26.00004044467077
25	26.276792296393253	24.069910451822125	23.72304224813541	25.93025500364921
26	25.946579943487762	24.29073835423281	24.00641231871136	25.756269383568064
27	26.45816826086077	24.46498134949159	23.534864225401826	25.541986164245806
28	26.0543098392692	24.874685864403727	23.650094769217194	25.42090952710988
29	26.07960614244245	24.84266103872945	23.757015771583205	25.32071704724489
30	26.242046647412554	24.727982013151873	23.494750465573315	25.535220873862265
31	26.15582596290488	24.28776015574875	23.79665154893897	25.759762332407394
32	25.714831979968857	24.545355940678697	23.727233786742605	26.012578292609838
33	26.06681091932575	24.300592146747974	23.522583752640394	26.110013181285886
34	26.10725559009694	24.23904271141073	24.04380525523345	25.609896443258883
35	26.088393666364556	24.235733601983995	23.93659010980728	25.73928262184417
36	25.851976181765707	24.031561550354535	24.12638591937171	25.99007634850805
37	25.567135395888986	23.926956924587234	24.454355431443524	26.051552248080256
38	25.580739512421108	23.75988366641971	24.609368824144735	26.050007997014447
39	25.191551475954725	23.826654141074687	24.972231056727328	26.009563326243264
40	25.53275742573347	23.513832996600808	25.297994495847988	25.65541508181773
41	25.207435201203044	23.37735061668931	25.31325316709348	26.10196101501416
42	24.861375890931754	23.495963805696448	25.35759523341171	26.28506506996009
43	25.623610863438568	22.55202195777944	24.87001634332378	26.954350835458214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	102.5
2	109.0
3	178.5
4	248.0
5	248.0
6	361.5
7	475.0
8	574.0
9	673.0
10	1029.0
11	1385.0
12	1385.0
13	3060.0
14	4735.0
15	9373.0
16	14011.0
17	10532.0
18	7053.0
19	7053.0
20	8230.5
21	9408.0
22	8668.5
23	7929.0
24	9886.5
25	11844.0
26	11844.0
27	15301.5
28	18759.0
29	22939.5
30	27120.0
31	33696.5
32	40273.0
33	40273.0
34	48360.5
35	56448.0
36	67568.0
37	78688.0
38	92277.5
39	105867.0
40	105867.0
41	121123.5
42	136380.0
43	152723.5
44	169067.0
45	187068.5
46	205070.0
47	205070.0
48	222477.0
49	239884.0
50	249573.0
51	259262.0
52	259234.5
53	259207.0
54	259207.0
55	249930.5
56	240654.0
57	225322.0
58	209990.0
59	193885.5
60	177781.0
61	177781.0
62	157616.0
63	137451.0
64	118276.5
65	99102.0
66	83924.0
67	68746.0
68	68746.0
69	57660.5
70	46575.0
71	39040.0
72	31505.0
73	26097.5
74	20690.0
75	20690.0
76	17155.5
77	13621.0
78	11086.0
79	8551.0
80	6848.5
81	5146.0
82	5146.0
83	4026.0
84	2906.0
85	2219.0
86	1532.0
87	1178.5
88	825.0
89	825.0
90	626.5
91	428.0
92	301.0
93	174.0
94	120.5
95	67.0
96	67.0
97	45.0
98	23.0
99	15.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2719765.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.25915143913798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.266390697891	63.78054492425025
2	6.4274873472046465	9.288895631896075
3	1.9991298964921436	4.333662898114021
4	0.9835237227720492	2.8427435851108105
5	0.5518382805642796	1.9937682942603896
6	0.3515268050980217	1.5240617186696572
7	0.27021897162227565	1.36680555145275
8	0.19657758828652167	1.1363623777229028
9	0.14219722987562217	0.9247546051027647
>10	0.7745412125373633	9.635767094918208
>50	0.02714447521357304	1.3213465711499772
>100	0.008863495667990325	1.071359230093691
>500	3.056055133718753E-4	0.15730311022494495
>1k	2.037370089133407E-4	0.2964247191729333
>5k	5.093425222833517E-5	0.32619968786065406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8863	0.3258737427682171	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2766	0.10169996304827808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.676788251926177E-5
2	0.0	0.0	0.0	0.0	3.676788251926177E-5
3	0.0	0.0	0.0	0.0	3.676788251926177E-5
4	0.0	0.0	0.0	0.0	3.676788251926177E-5
5	0.0	0.0	0.0	0.0	3.676788251926177E-5
6	0.0	0.0	0.0	0.0	3.676788251926177E-5
7	3.676788251926177E-5	0.0	0.0	0.0	3.676788251926177E-5
8	3.676788251926177E-5	0.0	0.0	0.0	3.676788251926177E-5
9	3.676788251926177E-5	0.0	0.0	0.0	3.676788251926177E-5
10	3.676788251926177E-5	3.676788251926177E-5	0.0	0.0	3.676788251926177E-5
11	3.676788251926177E-5	3.676788251926177E-5	0.0	0.0	3.676788251926177E-5
12	3.676788251926177E-5	3.676788251926177E-5	0.0	0.0	3.676788251926177E-5
13	3.676788251926177E-5	3.676788251926177E-5	0.0	0.0	3.676788251926177E-5
14	3.676788251926177E-5	3.676788251926177E-5	0.0	0.0	3.676788251926177E-5
15	3.676788251926177E-5	3.676788251926177E-5	0.0	3.676788251926177E-5	3.676788251926177E-5
16	3.676788251926177E-5	3.676788251926177E-5	0.0	3.676788251926177E-5	3.676788251926177E-5
17	3.676788251926177E-5	3.676788251926177E-5	0.0	3.676788251926177E-5	3.676788251926177E-5
18	3.676788251926177E-5	3.676788251926177E-5	0.0	3.676788251926177E-5	3.676788251926177E-5
19	3.676788251926177E-5	3.676788251926177E-5	0.0	1.1030364755778532E-4	7.353576503852355E-5
20	3.676788251926177E-5	3.676788251926177E-5	0.0	2.2060729511557065E-4	7.353576503852355E-5
21	1.470715300770471E-4	3.676788251926177E-5	0.0	4.7798247275040307E-4	1.1030364755778532E-4
22	1.8383941259630888E-4	3.676788251926177E-5	0.0	9.191970629815443E-4	1.1030364755778532E-4
23	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.0011030364755778533	2.2060729511557065E-4
24	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.0015442510658089945	2.2060729511557065E-4
25	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.0018383941259630886	2.2060729511557065E-4
26	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.0022060729511557066	2.2060729511557065E-4
27	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.004301842254753627	2.2060729511557065E-4
28	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.015883725248321087	2.2060729511557065E-4
29	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.04470974514342232	2.2060729511557065E-4
30	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.08761786404340081	2.2060729511557065E-4
31	1.8383941259630888E-4	3.676788251926177E-5	0.0	0.23410110800013972	2.2060729511557065E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACT	230	0.0	16.891304	4
TATACTG	310	0.0	16.709679	5
CGTATAC	80	3.3846905E-4	16.1875	3
ATTATAC	190	2.7284841E-11	15.578948	3
TTATACT	310	0.0	15.5161295	4
TCTTATA	1410	0.0	15.482269	37
CTTATAC	820	0.0	15.341464	37
TAAGGGT	320	0.0	15.031251	4
TATTAGT	150	8.113602E-8	14.8	2
TAGTACG	115	2.2119291E-5	14.478261	2
CGCCTAA	90	8.279138E-4	14.388889	22
GTAAGAC	260	0.0	14.230769	3
GAGTACT	2800	0.0	14.073215	11
ACCGTGC	305	0.0	13.95082	8
AGTACTT	2835	0.0	13.768959	12
GTACTTT	2805	0.0	13.71836	13
TATACCG	95	0.0012458389	13.631579	5
TTTATAC	205	1.4260877E-9	13.536586	3
TAAGACT	400	0.0	13.4125	4
AACGTTA	85	0.009409048	13.058824	12
>>END_MODULE