##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727297.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5803084
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.948563384572754	33.0	31.0	34.0	30.0	34.0
2	32.10034095663616	33.0	31.0	34.0	30.0	34.0
3	32.18040872749731	34.0	31.0	34.0	30.0	34.0
4	35.80514964112186	37.0	35.0	37.0	35.0	37.0
5	35.65356403595054	37.0	35.0	37.0	33.0	37.0
6	35.67346104243882	37.0	35.0	37.0	33.0	37.0
7	35.6147979246897	37.0	35.0	37.0	33.0	37.0
8	35.57948290943229	37.0	35.0	37.0	33.0	37.0
9	37.285808890583006	39.0	37.0	39.0	34.0	39.0
10	37.09911419514176	39.0	37.0	39.0	33.0	39.0
11	37.21629619698767	39.0	37.0	39.0	34.0	39.0
12	37.115553902028644	39.0	37.0	39.0	33.0	39.0
13	37.17304419512108	39.0	37.0	39.0	34.0	39.0
14	38.280299750960005	40.0	38.0	41.0	34.0	41.0
15	38.234754485718284	40.0	38.0	41.0	33.0	41.0
16	38.148314068864074	40.0	38.0	41.0	33.0	41.0
17	38.09726879707411	40.0	37.0	41.0	33.0	41.0
18	38.054311466110086	40.0	37.0	41.0	33.0	41.0
19	38.06227447336623	40.0	37.0	41.0	33.0	41.0
20	37.944280144833336	40.0	37.0	41.0	33.0	41.0
21	37.85641910404881	40.0	37.0	41.0	33.0	41.0
22	37.78166695501909	40.0	37.0	41.0	33.0	41.0
23	37.65388489982223	39.0	37.0	41.0	32.0	41.0
24	37.54221186527715	39.0	37.0	41.0	32.0	41.0
25	37.450161328011106	39.0	37.0	41.0	32.0	41.0
26	37.07060780095549	39.0	36.0	40.0	31.0	41.0
27	36.83453022565243	39.0	36.0	40.0	30.0	41.0
28	36.66325388362464	39.0	36.0	40.0	30.0	41.0
29	36.607161467936706	39.0	36.0	40.0	30.0	41.0
30	36.51458603735531	39.0	36.0	40.0	30.0	41.0
31	36.305925263187646	39.0	35.0	40.0	30.0	41.0
32	36.18529371623778	38.0	35.0	40.0	29.0	41.0
33	35.99981147955122	38.0	35.0	40.0	28.0	41.0
34	35.84112482259433	38.0	35.0	40.0	27.0	41.0
35	35.67538484709165	38.0	35.0	40.0	27.0	41.0
36	35.50573901739144	38.0	34.0	40.0	26.0	41.0
37	35.259348822109075	38.0	34.0	40.0	25.0	41.0
38	35.14544421552402	38.0	34.0	40.0	25.0	41.0
39	34.97207588241011	38.0	34.0	40.0	24.0	41.0
40	34.82223590077276	38.0	33.0	40.0	24.0	41.0
41	34.67708756240647	38.0	33.0	40.0	24.0	41.0
42	34.46215184891344	38.0	33.0	40.0	23.0	41.0
43	31.06498785818024	35.0	27.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	17.0
11	20.0
12	17.0
13	22.0
14	51.0
15	101.0
16	206.0
17	393.0
18	883.0
19	1808.0
20	3597.0
21	6553.0
22	11371.0
23	17853.0
24	27086.0
25	37966.0
26	50622.0
27	66141.0
28	85353.0
29	108322.0
30	135421.0
31	165552.0
32	205316.0
33	256621.0
34	327150.0
35	430341.0
36	599193.0
37	934225.0
38	1547418.0
39	783462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30252396828996	18.686718992866552	12.815375410729882	26.1953816281136
2	18.970361276865887	21.40765151771024	33.915294005739014	25.706693199684853
3	19.237650187383124	22.262834727189887	30.241161423822227	28.25835366160476
4	15.351974915407048	15.46510441689281	32.61676032950755	36.56616033819259
5	15.032817033149959	36.71365087942894	33.500204374087986	14.753327713333118
6	37.16766808820965	34.553747627985395	13.538266893948114	14.740317389856841
7	30.424322653265058	30.453479563625134	19.875483449834604	19.246714333275204
8	28.543443451792182	34.81092122740253	18.47109226749087	18.17454305331441
9	27.185424164116874	13.630907289985808	17.609395280164822	41.5742732657325
10	15.955688389139292	27.521073277588258	33.23012384449372	23.293114488778723
11	37.692337384742316	20.87369405647066	20.138791718334595	21.29517684045242
12	21.961753440067387	24.932036138025918	29.185257356260912	23.920953065645783
13	32.8139313509851	19.030984214600373	22.969786410122616	25.185298024291914
14	22.856691373069907	19.81954767499488	25.18242024413226	32.141340707802954
15	26.925217694591357	27.362330098961174	20.97455421979072	24.73789798665675
16	25.599353722951452	25.430719252039086	23.607430118192326	25.362496906817135
17	25.013337735590248	25.91142916421682	24.712049661869447	24.363183438323485
18	24.993761937617997	25.072271916105297	25.227672044726564	24.706294101550142
19	25.800057348816598	25.43326962008477	24.917405986196307	23.84926704490233
20	25.295291262370146	24.956626511006906	24.81342679168525	24.9346554349377
21	24.95990063214663	25.574022364659893	24.640467034425143	24.82560996876833
22	25.70157523137697	24.930175058641233	23.90951087387327	25.458738836108523
23	25.20177202328969	24.71597860723712	24.215193162807914	25.867056206665286
24	25.122452130625717	24.852457762114074	24.361770396568446	25.663319710691763
25	25.51476077203087	24.288188832007258	24.268871517282882	25.92817887867899
26	25.044700369665506	24.694955303076778	24.987179230905497	25.27316509635222
27	25.969019231842932	24.916372053204814	24.39418419585172	24.720424519100533
28	25.334718573779046	25.239993079541843	24.43090604926622	24.994382297412894
29	25.21066384701652	25.196430036166976	24.856266081966073	24.736640034850435
30	25.242543447587522	25.087298408914982	24.683047841458094	24.987110302039397
31	25.257725030345934	24.494561857109083	24.59004556887338	25.657667543671607
32	24.775619308629686	24.839344045338652	24.541416253840197	25.843620392191465
33	25.263273804066944	24.58155008612662	24.433129005197927	25.722047104608514
34	25.59063422138987	24.49116711045368	24.85059668272939	25.067601985427057
35	25.17132269669024	24.649893056864247	24.970532909742474	25.208251336703036
36	24.94633887774156	24.349311504020964	25.188572145431635	25.51577747280584
37	24.48215466121118	23.98517753663397	25.643106320708092	25.88956148144676
38	24.501058402738956	23.84466604309019	26.001191780094864	25.65308377407599
39	24.053554971804648	23.684647680440264	26.412886665090497	25.848910682664595
40	24.574950147197594	23.472346772853882	26.831956938758772	25.120746141189755
41	24.22353355560595	23.308468393702384	26.97077967508311	25.497218375608554
42	23.64325589634753	23.507569423430713	27.20084699790663	25.648327682315124
43	24.006045750845583	22.658469186384345	26.98544429134577	26.3500407714243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	478.0
1	515.5
2	553.0
3	1233.0
4	1913.0
5	1913.0
6	2284.0
7	2655.0
8	2817.0
9	2979.0
10	4202.5
11	5426.0
12	5426.0
13	11192.0
14	16958.0
15	29163.0
16	41368.0
17	30643.5
18	19919.0
19	19919.0
20	24510.5
21	29102.0
22	26440.5
23	23779.0
24	29834.5
25	35890.0
26	35890.0
27	45306.5
28	54723.0
29	64900.5
30	75078.0
31	89569.0
32	104060.0
33	104060.0
34	121689.5
35	139319.0
36	162590.0
37	185861.0
38	214408.5
39	242956.0
40	242956.0
41	272151.5
42	301347.0
43	332691.0
44	364035.0
45	397570.0
46	431105.0
47	431105.0
48	468009.0
49	504913.0
50	524906.5
51	544900.0
52	553411.0
53	561922.0
54	561922.0
55	530403.0
56	498884.0
57	453961.5
58	409039.0
59	378727.0
60	348415.0
61	348415.0
62	307680.5
63	266946.0
64	228043.0
65	189140.0
66	160368.5
67	131597.0
68	131597.0
69	109904.0
70	88211.0
71	75206.0
72	62201.0
73	51847.0
74	41493.0
75	41493.0
76	34884.0
77	28275.0
78	23562.5
79	18850.0
80	15323.0
81	11796.0
82	11796.0
83	9499.5
84	7203.0
85	5830.0
86	4457.0
87	3660.0
88	2863.0
89	2863.0
90	2137.0
91	1411.0
92	1016.5
93	622.0
94	462.5
95	303.0
96	303.0
97	203.5
98	104.0
99	69.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5803084.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.43175487637063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.38768693123197	45.114623119268444
2	9.608305363781213	10.652104554048746
3	3.279802980297422	5.454157045399097
4	1.5852436051730427	3.5149133976514473
5	0.910079034894082	2.5223638990186354
6	0.5889623781423724	1.9588330905955373
7	0.40438297179149774	1.5690960437967219
8	0.30974995930872523	1.3735987053893628
9	0.2382514178460055	1.188602477368878
>10	1.5330979280706096	15.9049373231866
>50	0.11176095411364459	4.22488727725118
>100	0.040035457800636096	3.7939953492709613
>500	0.001553544314807216	0.6282300552713467
>1k	9.01049251132892E-4	1.0098886613441642
>5k	1.5535331912776211E-4	0.6268660482101557
>10k+	3.107066382555243E-5	0.46290295292865763
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26877	0.46315028353889076	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8327	0.14349266700258	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8141	0.14028747472895448	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	7561	0.13029278914453074	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	6345	0.10933841385029064	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6023	0.10378964012928299	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4464433049737E-5
2	0.0	0.0	0.0	0.0	3.4464433049737E-5
3	0.0	0.0	0.0	0.0	3.4464433049737E-5
4	0.0	0.0	0.0	0.0	5.1696649574605504E-5
5	0.0	3.4464433049737E-5	0.0	0.0	5.1696649574605504E-5
6	0.0	3.4464433049737E-5	0.0	0.0	6.8928866099474E-5
7	0.0	3.4464433049737E-5	0.0	0.0	6.8928866099474E-5
8	1.72322165248685E-5	3.4464433049737E-5	0.0	0.0	6.8928866099474E-5
9	1.72322165248685E-5	3.4464433049737E-5	0.0	0.0	1.0339329914921101E-4
10	3.4464433049737E-5	3.4464433049737E-5	0.0	0.0	1.0339329914921101E-4
11	5.1696649574605504E-5	3.4464433049737E-5	0.0	0.0	1.0339329914921101E-4
12	5.1696649574605504E-5	3.4464433049737E-5	0.0	0.0	1.206255156740795E-4
13	5.1696649574605504E-5	5.1696649574605504E-5	0.0	0.0	1.206255156740795E-4
14	1.0339329914921101E-4	5.1696649574605504E-5	0.0	0.0	1.550899487238165E-4
15	1.206255156740795E-4	5.1696649574605504E-5	0.0	5.1696649574605504E-5	1.550899487238165E-4
16	1.37857732198948E-4	5.1696649574605504E-5	0.0	1.0339329914921101E-4	2.240188148232905E-4
17	1.37857732198948E-4	6.8928866099474E-5	0.0	3.618765470222385E-4	2.240188148232905E-4
18	1.37857732198948E-4	6.8928866099474E-5	0.0	3.963409800719755E-4	2.929476809227645E-4
19	1.550899487238165E-4	6.8928866099474E-5	0.0	6.375920114201346E-4	2.929476809227645E-4
20	1.550899487238165E-4	8.61610826243425E-5	0.0	0.0011545585071661897	2.929476809227645E-4
21	1.550899487238165E-4	8.61610826243425E-5	0.0	0.0016370605698625075	3.10179897447633E-4
22	1.550899487238165E-4	8.61610826243425E-5	0.0	0.002722690210929223	3.2741211397250154E-4
23	1.550899487238165E-4	8.61610826243425E-5	0.0	0.0034636755214985685	3.4464433049737E-4
24	1.550899487238165E-4	8.61610826243425E-5	0.0	0.004790556193913443	3.4464433049737E-4
25	1.550899487238165E-4	8.61610826243425E-5	1.72322165248685E-5	0.005738328102781211	3.618765470222385E-4
26	1.550899487238165E-4	8.61610826243425E-5	1.72322165248685E-5	0.006841189960372795	4.1357319659684403E-4
27	1.550899487238165E-4	8.61610826243425E-5	1.72322165248685E-5	0.010735670894993075	4.1357319659684403E-4
28	1.550899487238165E-4	8.61610826243425E-5	1.72322165248685E-5	0.03187960057100673	4.1357319659684403E-4
29	1.550899487238165E-4	8.61610826243425E-5	1.72322165248685E-5	0.08018150349021314	4.1357319659684403E-4
30	1.550899487238165E-4	8.61610826243425E-5	1.72322165248685E-5	0.1557964696013361	4.1357319659684403E-4
31	1.550899487238165E-4	8.61610826243425E-5	1.72322165248685E-5	0.4110745252007381	4.1357319659684403E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2930	0.0	17.99488	37
TCTATAC	515	0.0	15.446601	3
TCTTATA	4790	0.0	15.101253	37
GAGTACT	7915	0.0	14.024005	11
CTCTTAT	7735	0.0	13.728507	37
AGTACTT	8180	0.0	13.501834	12
GTACTTT	8400	0.0	13.12619	13
TAGACAG	960	0.0	12.718749	5
TACTTTT	8830	0.0	12.591733	14
TAGTACT	485	0.0	12.587629	4
TAACCCG	240	1.1314114E-9	12.333333	5
TTATACT	760	0.0	12.171053	4
ACTTTTT	9285	0.0	12.054389	15
TACTTAC	860	0.0	12.046512	31
GTATTAG	545	0.0	11.8807335	1
TCTACAC	1045	0.0	11.684212	3
GGGTAAG	2270	0.0	11.572687	1
TATACTG	720	0.0	11.562501	5
TATACAG	800	0.0	11.562499	5
TAAGACA	865	0.0	11.549133	4
>>END_MODULE