FastQCFastQC Report
Thu 9 Feb 2017
SRR2727296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5837090
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100620.1723804155837926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC30250.019.5702537
TCTTATA51250.014.83609837
TATACCG2500.014.7999995
GTCTTAG7250.014.2896561
AACGCAG72550.014.1778085
CTCTTAT84150.013.93820637
GGTATCA47750.013.4827221
GTATTAG5250.013.03809451
TCGTTAC5450.012.89908223
TATACAG7750.012.6516125
ACGCAGA81050.012.6224566
AGAGTAC83350.012.27414510
GTTACGG9400.012.20212825
CAACGCA85950.012.0535194
CGCAGAG85600.012.0163557
GTACTAG3550.011.9859161
TAATACC5100.011.9705884
CTCGTTA5600.011.89285822
CAGAGTA88150.011.8786169
TACGGAT11650.011.75107327
Produced by FastQC (version 0.11.5)