##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727296.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5837090
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16520423704277	33.0	31.0	34.0	30.0	34.0
2	32.3144784473085	34.0	31.0	34.0	30.0	34.0
3	32.3930989585564	34.0	31.0	34.0	30.0	34.0
4	35.97013888769918	37.0	35.0	37.0	35.0	37.0
5	35.845108264563336	37.0	35.0	37.0	35.0	37.0
6	35.852130256686124	37.0	35.0	37.0	35.0	37.0
7	35.79951653991972	37.0	35.0	37.0	35.0	37.0
8	35.76212342108825	37.0	35.0	37.0	35.0	37.0
9	37.499346934859666	39.0	37.0	39.0	35.0	39.0
10	37.33042098031725	39.0	37.0	39.0	34.0	39.0
11	37.43323299794932	39.0	37.0	39.0	35.0	39.0
12	37.33772444831243	39.0	37.0	39.0	34.0	39.0
13	37.393193183589766	39.0	37.0	39.0	34.0	39.0
14	38.58267561404741	40.0	38.0	41.0	34.0	41.0
15	38.538297165197044	40.0	38.0	41.0	34.0	41.0
16	38.46969740058831	40.0	38.0	41.0	34.0	41.0
17	38.41790875247769	40.0	38.0	41.0	34.0	41.0
18	38.35741610973961	40.0	38.0	41.0	34.0	41.0
19	38.353780393997695	40.0	38.0	41.0	34.0	41.0
20	38.23294329880129	40.0	38.0	41.0	34.0	41.0
21	38.14945580760276	40.0	38.0	41.0	33.0	41.0
22	38.072691529512134	40.0	38.0	41.0	33.0	41.0
23	37.95445692973725	40.0	37.0	41.0	33.0	41.0
24	37.84279306983446	40.0	37.0	41.0	33.0	41.0
25	37.753074048883946	40.0	37.0	41.0	32.0	41.0
26	37.387914011947736	39.0	37.0	41.0	32.0	41.0
27	37.17527997683777	39.0	37.0	41.0	31.0	41.0
28	37.01316923329947	39.0	36.0	40.0	31.0	41.0
29	36.97947727377854	39.0	36.0	40.0	30.0	41.0
30	36.90479125728745	39.0	36.0	40.0	30.0	41.0
31	36.70482021007043	39.0	36.0	40.0	30.0	41.0
32	36.59446916185976	39.0	36.0	40.0	30.0	41.0
33	36.41524201271524	39.0	36.0	40.0	30.0	41.0
34	36.28081852429892	39.0	35.0	40.0	29.0	41.0
35	36.12874206154094	39.0	35.0	40.0	28.0	41.0
36	35.981256071090215	39.0	35.0	40.0	27.0	41.0
37	35.74491347572164	38.0	35.0	40.0	27.0	41.0
38	35.63912411835349	38.0	35.0	40.0	26.0	41.0
39	35.47292674945906	38.0	34.0	40.0	26.0	41.0
40	35.34579028248665	38.0	34.0	40.0	25.0	41.0
41	35.2165836058721	38.0	34.0	40.0	25.0	41.0
42	35.032074372675424	38.0	34.0	40.0	24.0	41.0
43	31.517001793701997	35.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	4.0
12	14.0
13	16.0
14	26.0
15	67.0
16	155.0
17	300.0
18	641.0
19	1293.0
20	2691.0
21	5288.0
22	9389.0
23	15162.0
24	22854.0
25	32491.0
26	43805.0
27	58132.0
28	74272.0
29	94930.0
30	119076.0
31	149279.0
32	185890.0
33	232242.0
34	298131.0
35	392992.0
36	550152.0
37	857004.0
38	1577634.0
39	1113153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78604578651349	18.07121356703426	12.064350558240493	27.078390088211762
2	19.353976039430606	20.3350299549947	33.71549864744247	26.595495358132222
3	18.980056843392855	21.251668211386153	29.96789496135917	29.80037998386182
4	15.36279207618865	15.237609836408211	32.52978795941128	36.86981012799186
5	15.019864350215602	36.195689975655675	33.844261438490754	14.940184235637963
6	37.71279867194098	33.96070644790469	13.77057746239993	14.555917417754394
7	31.25271325266528	30.085470671173482	19.447224558812696	19.21459151734854
8	28.648453253247762	34.186486759669634	18.28971285349378	18.875347133588825
9	27.41429376624311	13.19649003184806	17.355377422654097	42.03383877925474
10	15.97359300610407	26.974622628741372	33.284718926725475	23.76706543842908
11	38.458529849633976	20.23861204812672	20.379401379797123	20.923456722442175
12	22.351342878043685	24.103174698351403	29.057338502575767	24.488143921029142
13	32.48137342408632	18.963421842048007	22.633778132596895	25.92142660126878
14	23.213776042514336	19.3684866945687	24.179805348212895	33.23793191470407
15	26.57329251390676	27.072685190737168	20.50749602969973	25.846526265656344
16	26.2002984363784	25.353198254609744	22.701911397631356	25.7445919113805
17	25.453916249363985	25.427498976373503	23.873762439845883	25.244822334416632
18	25.498664574299866	24.851338595087622	24.255014056661793	25.39498277395072
19	26.487376415302833	24.70799662160426	24.110284405414344	24.69434255767857
20	26.12068684909775	24.432653942289736	23.975508344055	25.47115086455751
21	25.9689674135571	24.571455982347366	23.975422684933758	25.484153919161773
22	26.49357813568062	24.154381721028802	23.49136299080535	25.860677152485227
23	25.94864906999892	24.232742685139343	23.765163805937547	26.05344443892419
24	25.850209607869672	24.104716562533728	23.771211339897107	26.27386248969949
25	26.146230399051586	23.804961033665748	23.82428573141754	26.224522835865134
26	25.805598337527773	24.11660604856187	24.079018826161665	25.99877678774869
27	26.165863469639838	24.38293738832192	23.714008178732897	25.737190963305345
28	25.948255038041218	24.472468301842184	23.725829137464043	25.853447522652555
29	25.791670164414114	24.68680455500943	23.912805867307167	25.60871941326928
30	26.16154624992933	24.42965587304633	23.633437209294357	25.77536066772998
31	26.01787877178526	23.980219595723213	23.833554048335728	26.168347584155804
32	25.55746099511914	24.360854466866193	23.88697107634112	26.19471346167354
33	25.710431053829907	23.89783265291438	23.946230056415096	26.445506236840615
34	25.95836281434756	23.78549928131997	24.333734789081547	25.92240311525092
35	25.777005322857793	23.79514449837162	24.338703018113478	26.089147160657106
36	25.549751674207528	23.429585632566912	24.749027340678317	26.271635352547246
37	25.320664920362717	23.155305126355767	25.028601580582105	26.495428372699408
38	25.065195157175925	23.089758766782765	25.404045508977934	26.441000567063384
39	24.720554248778072	22.978847336600943	25.941419440166246	26.35917897445474
40	25.078814957453115	22.75985122723823	26.167011301864456	25.994322513444203
41	24.51257390240685	22.703744502825895	26.535859477924788	26.247822116842467
42	24.16724772103908	22.85870185314943	26.533323967936077	26.440726457875414
43	24.344819079370026	21.99587465672107	26.412835847999606	27.246470415909297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	193.0
2	221.0
3	545.0
4	869.0
5	869.0
6	990.5
7	1112.0
8	1127.0
9	1142.0
10	1751.0
11	2360.0
12	2360.0
13	4663.0
14	6966.0
15	11631.0
16	16296.0
17	12821.5
18	9347.0
19	9347.0
20	12038.5
21	14730.0
22	14957.0
23	15184.0
24	20076.5
25	24969.0
26	24969.0
27	31874.0
28	38779.0
29	47615.5
30	56452.0
31	70158.5
32	83865.0
33	83865.0
34	101528.0
35	119191.0
36	143092.5
37	166994.0
38	196571.0
39	226148.0
40	226148.0
41	259137.0
42	292126.0
43	328367.5
44	364609.0
45	404726.0
46	444843.0
47	444843.0
48	481663.5
49	518484.0
50	540096.0
51	561708.0
52	561785.0
53	561862.0
54	561862.0
55	543431.5
56	525001.0
57	495083.0
58	465165.0
59	431966.5
60	398768.0
61	398768.0
62	350855.0
63	302942.0
64	257857.0
65	212772.0
66	178111.5
67	143451.0
68	143451.0
69	118153.5
70	92856.0
71	77042.5
72	61229.0
73	50296.5
74	39364.0
75	39364.0
76	32250.0
77	25136.0
78	20948.0
79	16760.0
80	13529.5
81	10299.0
82	10299.0
83	8345.5
84	6392.0
85	5110.5
86	3829.0
87	3128.0
88	2427.0
89	2427.0
90	1840.0
91	1253.0
92	923.5
93	594.0
94	434.5
95	275.0
96	275.0
97	187.0
98	99.0
99	77.5
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5837090.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.03371085747221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0348052486458	47.02750457191481
2	10.030502837913337	11.642146029010341
3	3.3013623750375634	5.747709285260031
4	1.5977699983237046	3.7089808839784695
5	0.8860434825531419	2.5710195636818383
6	0.6022656002785142	2.0971024623579133
7	0.41346300735527247	1.6796354833381753
8	0.30350537661489985	1.409083461612584
9	0.24310002231165703	1.2697196763851772
>10	1.4561120696137893	15.719567285813474
>50	0.10101136930676824	3.9402619674870647
>100	0.0295864726044555	2.7148986249124527
>500	3.24598312181746E-4	0.12037211649651189
>1k	1.1803290327596941E-4	0.17968967070293673
>5k	0.0	0.0
>10k+	2.9508225818063358E-5	0.17230891704817486
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10062	0.1723804155837926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7131824248041405E-5
2	0.0	0.0	0.0	0.0	1.7131824248041405E-5
3	0.0	0.0	0.0	0.0	1.7131824248041405E-5
4	0.0	0.0	0.0	0.0	3.426364849608281E-5
5	0.0	0.0	0.0	0.0	3.426364849608281E-5
6	3.426364849608281E-5	0.0	0.0	0.0	6.852729699216562E-5
7	3.426364849608281E-5	0.0	0.0	0.0	6.852729699216562E-5
8	3.426364849608281E-5	0.0	0.0	0.0	8.565912124020702E-5
9	3.426364849608281E-5	0.0	0.0	0.0	1.7131824248041403E-4
10	5.1395472744124214E-5	0.0	0.0	0.0	1.7131824248041403E-4
11	5.1395472744124214E-5	0.0	0.0	0.0	1.7131824248041403E-4
12	5.1395472744124214E-5	0.0	0.0	0.0	2.2271371522453827E-4
13	5.1395472744124214E-5	0.0	0.0	1.7131824248041405E-5	2.569773637206211E-4
14	5.1395472744124214E-5	0.0	0.0	1.7131824248041405E-5	3.4263648496082806E-4
15	5.1395472744124214E-5	0.0	0.0	5.1395472744124214E-5	3.597683092088695E-4
16	5.1395472744124214E-5	0.0	0.0	8.565912124020702E-5	4.282956062010351E-4
17	5.1395472744124214E-5	0.0	0.0	1.1992276973628983E-4	4.282956062010351E-4
18	5.1395472744124214E-5	0.0	0.0	1.7131824248041403E-4	4.796910789451593E-4
19	6.852729699216562E-5	0.0	0.0	2.3984553947257965E-4	4.968229031932007E-4
20	6.852729699216562E-5	0.0	0.0	4.282956062010351E-4	5.139547274412422E-4
21	8.565912124020702E-5	0.0	0.0	6.338774971775319E-4	5.48218375937325E-4
22	8.565912124020702E-5	0.0	0.0	0.0012163595216109397	5.48218375937325E-4
23	8.565912124020702E-5	0.0	0.0	0.0014733368853315607	7.538002669138218E-4
24	1.0279094548824843E-4	0.0	0.0	0.0019701597885247615	7.538002669138218E-4
25	1.0279094548824843E-4	0.0	0.0	0.0022271371522453827	7.538002669138218E-4
26	1.0279094548824843E-4	0.0	0.0	0.002621169109950335	7.880639154099045E-4
27	1.0279094548824843E-4	0.0	0.0	0.0062359840262870714	8.05195739657946E-4
28	1.0279094548824843E-4	0.0	0.0	0.026502932111720053	8.05195739657946E-4
29	1.0279094548824843E-4	0.0	0.0	0.07178234359929349	8.05195739657946E-4
30	1.1992276973628983E-4	0.0	0.0	0.13758568053602052	8.05195739657946E-4
31	1.1992276973628983E-4	0.0	0.0	0.39147931589199414	8.223275639059874E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3025	0.0	19.57025	37
TCTTATA	5125	0.0	14.836098	37
TATACCG	250	0.0	14.799999	5
GTCTTAG	725	0.0	14.289656	1
AACGCAG	7255	0.0	14.177808	5
CTCTTAT	8415	0.0	13.938206	37
GGTATCA	4775	0.0	13.482722	1
GTATTAG	525	0.0	13.0380945	1
TCGTTAC	545	0.0	12.899082	23
TATACAG	775	0.0	12.651612	5
ACGCAGA	8105	0.0	12.622456	6
AGAGTAC	8335	0.0	12.274145	10
GTTACGG	940	0.0	12.202128	25
CAACGCA	8595	0.0	12.053519	4
CGCAGAG	8560	0.0	12.016355	7
GTACTAG	355	0.0	11.985916	1
TAATACC	510	0.0	11.970588	4
CTCGTTA	560	0.0	11.892858	22
CAGAGTA	8815	0.0	11.878616	9
TACGGAT	1165	0.0	11.751073	27
>>END_MODULE