FastQCFastQC Report
Thu 9 Feb 2017
SRR2727295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4900997
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.11560913014229553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19600.017.65050937
TCTTATA35150.017.21052637
CTCTTAT56400.014.104609537
AACGCAG53000.013.752835
TAGGACT7300.013.6849324
GGTATCA31650.013.6777251
TATACTG6150.013.2357725
TAATACC4650.012.7311824
CAACGCA57600.012.6223964
GGGTAAG13150.012.5209121
TCAACGC58700.012.4488933
ACGCAGA58800.012.3962586
ACTATAC3450.012.3333333
ATCAACG59650.012.18860152
ATACCGT3350.012.1492536
AGAGTAC60150.012.08728210
TAATACT4750.012.0736844
TATCAAC60850.011.8266231
TAAGAGT6750.011.7851864
GTCTTAG7750.011.69677451
Produced by FastQC (version 0.11.5)