##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4900997
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.133247173993375	33.0	31.0	34.0	30.0	34.0
2	32.28839050503397	34.0	31.0	34.0	30.0	34.0
3	32.36980700049398	34.0	31.0	34.0	30.0	34.0
4	35.95398548499418	37.0	35.0	37.0	35.0	37.0
5	35.82455059654189	37.0	35.0	37.0	35.0	37.0
6	35.832794429378346	37.0	35.0	37.0	35.0	37.0
7	35.78111902537382	37.0	35.0	37.0	35.0	37.0
8	35.74344546630002	37.0	35.0	37.0	35.0	37.0
9	37.47763648906539	39.0	37.0	39.0	35.0	39.0
10	37.311359300974885	39.0	37.0	39.0	34.0	39.0
11	37.40980600477821	39.0	37.0	39.0	35.0	39.0
12	37.31127564452702	39.0	37.0	39.0	34.0	39.0
13	37.362235887922395	39.0	37.0	39.0	34.0	39.0
14	38.55299584961998	40.0	38.0	41.0	34.0	41.0
15	38.506255359878814	40.0	38.0	41.0	34.0	41.0
16	38.436743176949506	40.0	38.0	41.0	34.0	41.0
17	38.37799635461928	40.0	38.0	41.0	34.0	41.0
18	38.31660149965405	40.0	38.0	41.0	34.0	41.0
19	38.309451525883404	40.0	38.0	41.0	34.0	41.0
20	38.183116210844446	40.0	38.0	41.0	34.0	41.0
21	38.09891864859334	40.0	38.0	41.0	33.0	41.0
22	38.016638247279076	40.0	37.0	41.0	33.0	41.0
23	37.89764449968037	40.0	37.0	41.0	33.0	41.0
24	37.781021494198015	40.0	37.0	41.0	32.0	41.0
25	37.68806550993604	40.0	37.0	41.0	32.0	41.0
26	37.31437787046187	39.0	37.0	41.0	31.0	41.0
27	37.09886376180194	39.0	36.0	41.0	31.0	41.0
28	36.93820032944317	39.0	36.0	40.0	30.0	41.0
29	36.90076100842339	39.0	36.0	40.0	30.0	41.0
30	36.825936641054874	39.0	36.0	40.0	30.0	41.0
31	36.61712912699192	39.0	36.0	40.0	30.0	41.0
32	36.50664732094307	39.0	36.0	40.0	30.0	41.0
33	36.32696857394526	39.0	35.0	40.0	29.0	41.0
34	36.18852633453969	39.0	35.0	40.0	29.0	41.0
35	36.03484270649421	39.0	35.0	40.0	28.0	41.0
36	35.88530986654348	38.0	35.0	40.0	27.0	41.0
37	35.63992612115453	38.0	35.0	40.0	26.0	41.0
38	35.52892380876789	38.0	35.0	40.0	26.0	41.0
39	35.362210178867684	38.0	34.0	40.0	25.0	41.0
40	35.23502503674253	38.0	34.0	40.0	25.0	41.0
41	35.100745011678235	38.0	34.0	40.0	24.0	41.0
42	34.91476122103319	38.0	34.0	40.0	24.0	41.0
43	31.382923311318084	35.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	16.0
12	13.0
13	26.0
14	37.0
15	67.0
16	135.0
17	262.0
18	603.0
19	1188.0
20	2586.0
21	4801.0
22	8587.0
23	13588.0
24	20614.0
25	28808.0
26	38756.0
27	50574.0
28	64562.0
29	81481.0
30	102826.0
31	127876.0
32	158773.0
33	198271.0
34	253666.0
35	334123.0
36	465372.0
37	720626.0
38	1318220.0
39	904533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.792435906408436	18.049184686299544	11.932082390582979	27.22629701670905
2	19.714886583280915	20.249206436975985	33.31175269031995	26.72415428942315
3	19.189687322803913	21.095544437182884	29.687775772970276	30.02699246704293
4	15.585339064684186	15.221943616778383	32.19865264149315	36.99406467704428
5	15.222596545151934	36.00408243465564	33.62285265630646	15.15046836388596
6	37.947891010747405	33.770700124892954	13.601293777572195	14.680115086787444
7	31.376840263317852	29.902099511589174	19.28019951858775	19.440860706505227
8	28.729440152687303	33.906896902813855	18.1895030745785	19.174159869920345
9	27.434234299674127	13.167239237240913	17.2287189728947	42.16980749019026
10	16.213721412194293	26.778204516346367	33.103958235436586	23.90411583602275
11	38.659011625593735	20.097380186113153	20.19001439911104	21.05359378918208
12	22.486587933026687	23.96138989679039	28.813525084793973	24.738497085388953
13	32.614323167306566	18.910988927354985	22.43368033075719	26.041007574581254
14	23.39936139524264	19.28073002289126	23.83874138262072	33.48116719924538
15	26.719706214878318	26.91025927989754	20.349696194468187	26.020338310755953
16	26.446659730662965	25.198301488452245	22.43488416744593	25.920154613438857
17	25.678509903189084	25.300586798971718	23.595627583530455	25.425275714308743
18	25.75149505294535	24.71433873556748	23.96957190547148	25.564594306015692
19	26.664737807429795	24.55510582846715	23.871653053450146	24.908503310652915
20	26.37834709957994	24.255044432796023	23.72033690287915	25.64627156474489
21	26.16075871909328	24.36973538241301	23.66863313729839	25.800872761195325
22	26.784652184035206	23.962165249233983	23.225519215784054	26.027663350946757
23	26.210728143681784	24.05441178601007	23.454554246819576	26.28030582348857
24	26.157392057167144	24.0340281783482	23.37279537204369	26.435784392440965
25	26.38852870140504	23.69903511469197	23.490424499341664	26.422011684561326
26	26.073225509013774	23.989404604818162	23.715541960135866	26.221827926032194
27	26.418624618623515	24.152004173844627	23.458859493282695	25.970511714249163
28	26.25008748220005	24.29672982864507	23.440312246671443	26.01287044248344
29	26.112421615438652	24.443169420426088	23.589587996075085	25.85482096806017
30	26.31474779519351	24.23725213461669	23.408216736308958	26.03978333388084
31	26.27198098672576	23.781691765981495	23.531783431003937	26.41454381628881
32	25.853882383523192	24.175407575234182	23.59691303626589	26.37379700497674
33	26.007402167354925	23.73831283716354	23.632375208554503	26.621909786927027
34	26.247618596787554	23.631967128321033	23.964776962728195	26.155637312163222
35	26.03774293271349	23.681548876687746	23.959288283588013	26.321419907010757
36	25.8425377530327	23.334599062190815	24.33641563135011	26.486447553426373
37	25.639170968682496	23.10346241795292	24.632885920966693	26.624480692397896
38	25.318073036975946	23.135578332327075	25.013563566759988	26.53278506393699
39	25.174469602817553	22.957961410708883	25.404810490600177	26.46275849587339
40	25.414624820215153	22.701095307750645	25.6117887850166	26.272491087017602
41	24.8967914079523	22.716235084412418	25.965390307319105	26.421583200316178
42	24.598586777343467	22.751921700829445	25.99165027034295	26.657841251484136
43	24.79617922639006	21.92876265788369	25.872756094321215	27.402302021405035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	170.0
2	174.0
3	366.0
4	558.0
5	558.0
6	628.0
7	698.0
8	742.0
9	786.0
10	1078.5
11	1371.0
12	1371.0
13	2887.0
14	4403.0
15	7021.0
16	9639.0
17	7883.5
18	6128.0
19	6128.0
20	8145.0
21	10162.0
22	10684.5
23	11207.0
24	14935.0
25	18663.0
26	18663.0
27	24257.5
28	29852.0
29	37259.5
30	44667.0
31	55897.5
32	67128.0
33	67128.0
34	81584.0
35	96040.0
36	115600.0
37	135160.0
38	159449.5
39	183739.0
40	183739.0
41	212164.5
42	240590.0
43	271588.0
44	302586.0
45	334529.5
46	366473.0
47	366473.0
48	397723.5
49	428974.0
50	448243.5
51	467513.0
52	469116.5
53	470720.0
54	470720.0
55	455874.5
56	441029.0
57	418451.5
58	395874.0
59	367261.5
60	338649.0
61	338649.0
62	300375.0
63	262101.0
64	224485.5
65	186870.0
66	157667.0
67	128464.0
68	128464.0
69	106688.5
70	84913.0
71	71604.5
72	58296.0
73	48519.0
74	38742.0
75	38742.0
76	32202.5
77	25663.0
78	21427.5
79	17192.0
80	13950.0
81	10708.0
82	10708.0
83	8618.5
84	6529.0
85	5184.0
86	3839.0
87	3142.0
88	2445.0
89	2445.0
90	1851.0
91	1257.0
92	923.0
93	589.0
94	432.0
95	275.0
96	275.0
97	194.5
98	114.0
99	82.5
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4900997.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19658357840384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.96278954858943	47.92720538253795
2	9.943608693698804	11.772553262149685
3	3.3859874985155423	6.0131667585391755
4	1.6305733184252633	3.8609747889950565
5	0.9336795157964197	2.763531874614319
6	0.6097413568645892	2.1656763115706408
7	0.4413207930708143	1.8287278248332748
8	0.312538398050141	1.4800964321308496
9	0.2441517597149738	1.3007655044803637
>10	1.4336782579023417	15.503017767317035
>50	0.08154204864029853	3.2314264103868626
>100	0.020043936728484202	1.8429324701448708
>500	2.069246837219302E-4	0.07394451655869354
>1k	1.0346198948683783E-4	0.12030748680711247
>5k	3.448732982894593E-5	0.11567320893417596
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5666	0.11560913014229553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0808023347086315E-5
2	0.0	0.0	0.0	0.0	4.0808023347086315E-5
3	0.0	0.0	0.0	2.0404011673543157E-5	4.0808023347086315E-5
4	0.0	0.0	0.0	2.0404011673543157E-5	8.161604669417263E-5
5	0.0	0.0	0.0	2.0404011673543157E-5	8.161604669417263E-5
6	4.0808023347086315E-5	0.0	0.0	2.0404011673543157E-5	1.0202005836771579E-4
7	4.0808023347086315E-5	0.0	0.0	4.0808023347086315E-5	1.0202005836771579E-4
8	4.0808023347086315E-5	0.0	0.0	4.0808023347086315E-5	1.2242407004125894E-4
9	4.0808023347086315E-5	0.0	0.0	4.0808023347086315E-5	1.8363610506188843E-4
10	4.0808023347086315E-5	0.0	0.0	4.0808023347086315E-5	1.8363610506188843E-4
11	4.0808023347086315E-5	0.0	0.0	4.0808023347086315E-5	1.8363610506188843E-4
12	4.0808023347086315E-5	2.0404011673543157E-5	0.0	4.0808023347086315E-5	1.8363610506188843E-4
13	4.0808023347086315E-5	2.0404011673543157E-5	0.0	4.0808023347086315E-5	1.8363610506188843E-4
14	6.121203502062947E-5	2.0404011673543157E-5	0.0	4.0808023347086315E-5	2.6525215175606106E-4
15	6.121203502062947E-5	4.0808023347086315E-5	0.0	4.0808023347086315E-5	2.6525215175606106E-4
16	6.121203502062947E-5	4.0808023347086315E-5	0.0	6.121203502062947E-5	3.264641867766905E-4
17	6.121203502062947E-5	4.0808023347086315E-5	0.0	1.0202005836771579E-4	3.264641867766905E-4
18	6.121203502062947E-5	4.0808023347086315E-5	0.0	1.0202005836771579E-4	3.6727221012377686E-4
19	6.121203502062947E-5	4.0808023347086315E-5	0.0	1.0202005836771579E-4	3.6727221012377686E-4
20	8.161604669417263E-5	4.0808023347086315E-5	0.0	1.4282808171480212E-4	3.6727221012377686E-4
21	1.0202005836771579E-4	4.0808023347086315E-5	0.0	2.6525215175606106E-4	3.6727221012377686E-4
22	1.0202005836771579E-4	4.0808023347086315E-5	0.0	6.121203502062948E-4	3.6727221012377686E-4
23	1.2242407004125894E-4	4.0808023347086315E-5	0.0	7.957564552681832E-4	4.488882568179495E-4
24	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.0012242407004125896	4.488882568179495E-4
25	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.0013874727938009348	4.6929226849149266E-4
26	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.0018771690739659706	4.6929226849149266E-4
27	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.004733730708262013	4.6929226849149266E-4
28	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.022628048945959364	4.6929226849149266E-4
29	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.05627426419563203	4.6929226849149266E-4
30	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.10785560570634914	4.6929226849149266E-4
31	1.2242407004125894E-4	4.0808023347086315E-5	0.0	0.30589694300975906	4.896962801650358E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1960	0.0	17.650509	37
TCTTATA	3515	0.0	17.210526	37
CTCTTAT	5640	0.0	14.1046095	37
AACGCAG	5300	0.0	13.75283	5
TAGGACT	730	0.0	13.684932	4
GGTATCA	3165	0.0	13.677725	1
TATACTG	615	0.0	13.235772	5
TAATACC	465	0.0	12.731182	4
CAACGCA	5760	0.0	12.622396	4
GGGTAAG	1315	0.0	12.520912	1
TCAACGC	5870	0.0	12.448893	3
ACGCAGA	5880	0.0	12.396258	6
ACTATAC	345	0.0	12.333333	3
ATCAACG	5965	0.0	12.1886015	2
ATACCGT	335	0.0	12.149253	6
AGAGTAC	6015	0.0	12.087282	10
TAATACT	475	0.0	12.073684	4
TATCAAC	6085	0.0	11.826623	1
TAAGAGT	675	0.0	11.785186	4
GTCTTAG	775	0.0	11.6967745	1
>>END_MODULE