FastQCFastQC Report
Thu 9 Feb 2017
SRR2727294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600644
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG186730.28289663857041825No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG119840.181558041912274No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG87770.13297187365354046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81790.123912151602177No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT69760.10568665724132373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC28700.018.9512237
TCTTATA48250.015.64352337
GTCTTAG10450.014.8708131
TATACCG3050.014.5573775
TAGTACT4950.014.2020214
CTCTTAT79850.013.69254937
TAGATCG2850.013.6315785
TATACTA2750.013.4545465
TTAGAAC6950.013.0431653
ATAGTAC5250.013.0380953
TTACACT8350.012.6287424
TAAGACT9550.012.3979054
TTAGTAC3300.012.3333333
GTATAAG5600.012.2232141
TAAGACA8700.012.120694
TAGGACA10000.012.0250014
TTAATAC5850.012.0170943
CTAAGAC8650.011.9768793
GTATAAT5100.011.9705881
TTAGCGC2951.6370905E-1111.9152554
Produced by FastQC (version 0.11.5)