##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727294.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6600644
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.118587368141654	33.0	31.0	34.0	30.0	34.0
2	32.26607161361831	33.0	31.0	34.0	30.0	34.0
3	32.34756957048433	34.0	31.0	34.0	30.0	34.0
4	35.93622834377979	37.0	35.0	37.0	35.0	37.0
5	35.80381353698215	37.0	35.0	37.0	35.0	37.0
6	35.81982545945517	37.0	35.0	37.0	35.0	37.0
7	35.761264052416706	37.0	35.0	37.0	35.0	37.0
8	35.717205775678856	37.0	35.0	37.0	33.0	37.0
9	37.45760716681584	39.0	37.0	39.0	35.0	39.0
10	37.28734984040951	39.0	37.0	39.0	34.0	39.0
11	37.39578244183446	39.0	37.0	39.0	34.0	39.0
12	37.292917175960405	39.0	37.0	39.0	34.0	39.0
13	37.34947196061475	39.0	37.0	39.0	34.0	39.0
14	38.50595502499453	40.0	38.0	41.0	34.0	41.0
15	38.462349582858884	40.0	38.0	41.0	34.0	41.0
16	38.38065270600869	40.0	38.0	41.0	34.0	41.0
17	38.30196099047305	40.0	38.0	41.0	34.0	41.0
18	38.25823162103577	40.0	38.0	41.0	33.0	41.0
19	38.24504078692927	40.0	38.0	41.0	34.0	41.0
20	38.12510355050204	40.0	38.0	41.0	33.0	41.0
21	38.030249624127585	40.0	37.0	41.0	33.0	41.0
22	37.95256205303604	40.0	37.0	41.0	33.0	41.0
23	37.821372278220124	40.0	37.0	41.0	33.0	41.0
24	37.6937436710721	40.0	37.0	41.0	32.0	41.0
25	37.605694838261236	40.0	37.0	41.0	32.0	41.0
26	37.206505001633175	39.0	37.0	41.0	31.0	41.0
27	36.984595594005675	39.0	36.0	40.0	30.0	41.0
28	36.79777215677743	39.0	36.0	40.0	30.0	41.0
29	36.75657875201268	39.0	36.0	40.0	30.0	41.0
30	36.64779466973223	39.0	36.0	40.0	30.0	41.0
31	36.45687784404067	39.0	36.0	40.0	30.0	41.0
32	36.32358797111312	39.0	35.0	40.0	29.0	41.0
33	36.13174275116186	39.0	35.0	40.0	28.0	41.0
34	35.97247829151216	39.0	35.0	40.0	27.0	41.0
35	35.80260077653029	38.0	35.0	40.0	27.0	41.0
36	35.629864146589334	38.0	35.0	40.0	26.0	41.0
37	35.39020798576624	38.0	34.0	40.0	25.0	41.0
38	35.27426323855672	38.0	34.0	40.0	25.0	41.0
39	35.10892028111196	38.0	34.0	40.0	24.0	41.0
40	34.96709411990709	38.0	34.0	40.0	24.0	41.0
41	34.81317201776069	38.0	33.0	40.0	24.0	41.0
42	34.595151927599794	38.0	33.0	40.0	23.0	41.0
43	31.01500899003188	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	15.0
11	18.0
12	21.0
13	27.0
14	50.0
15	117.0
16	211.0
17	421.0
18	935.0
19	1886.0
20	3737.0
21	7206.0
22	12789.0
23	20501.0
24	30307.0
25	42330.0
26	56692.0
27	73604.0
28	93365.0
29	116943.0
30	144396.0
31	180546.0
32	221566.0
33	275472.0
34	351540.0
35	459112.0
36	636672.0
37	980624.0
38	1741604.0
39	1147934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38393344649401	17.58129964288333	12.048278925510905	26.986487985111758
2	19.452298896895513	20.270885689335767	33.339413548132576	26.93740186563614
3	19.457252958953703	21.1556175427731	29.21470995860404	30.17241953966916
4	15.67718240826198	14.76772569464434	31.597735008887014	37.95735688820667
5	15.603007827721052	35.83376106937444	33.22139476087485	15.341836342029659
6	38.718828041627454	33.34247385558137	13.024592751858757	14.914105350932424
7	31.826712666218633	29.583946657326166	18.9940254314579	19.595315244997305
8	29.25981161838148	34.05881304915096	17.892678350779107	18.788696981688453
9	27.524677895065995	13.362393124064864	16.593017287404077	42.51991169346506
10	16.502753367701697	26.669549213682785	32.62148360069108	24.206213817924432
11	38.83699832925394	19.84676040701483	19.402712826203018	21.913528437528218
12	22.848285712727424	24.069727135715848	28.283058440964243	24.798928710592484
13	33.94343036830952	18.07134273564822	21.79443399765235	26.190792898389915
14	23.463983211335137	18.887202521450938	23.953268802256265	33.69554546495766
15	28.01890239800844	26.150478650265036	19.885605101562817	25.94501385016371
16	26.97214090019095	24.453841170649408	22.306868844918768	26.26714908424087
17	26.347186729052495	24.833713195257918	23.633315173489134	25.185784902200453
18	26.469462676672155	24.16093035770449	23.46589211598141	25.903714849641947
19	26.894587861426857	24.62993004924974	23.580305194462845	24.895176894860562
20	26.781765536817314	23.856293416218175	23.048281349516806	26.313659697447704
21	26.10608904222073	24.75679645804258	22.937534579959166	26.199579919777527
22	27.1896196795343	24.125827722264674	22.209696508401304	26.47485608979972
23	26.39684854992937	23.591773772377362	22.508470385616917	27.50290729207635
24	26.648536112536895	24.012505446438258	22.700815253784327	26.638143187240516
25	26.80349068969634	23.004376542652505	22.52449912463087	27.667633643020288
26	26.302857721155693	23.830023252276597	23.377279550298425	26.48983947626928
27	27.536373723533643	23.454756838878147	22.80868351633568	26.200185921252533
28	26.559044844715153	24.370864418683997	22.871995520437096	26.198095216163757
29	26.91314665659896	23.96148012224262	23.025662344462148	26.099710876696275
30	26.277693509906	24.039563412297348	23.25147364408685	26.431269433709804
31	26.666034405127743	23.623891850552763	22.785049458810384	26.925024285509107
32	26.029520755853518	23.563625003863258	22.937761830512297	27.469092409770923
33	26.52191210433406	23.63389087489039	22.99239286348423	26.85180415729132
34	27.2076330733789	23.44610616782241	22.917491081173292	26.428769677625397
35	26.48982432623241	23.500100899245588	23.427380722244678	26.582694052277322
36	26.282102170636684	23.50520646167253	23.60315145007063	26.60953991762016
37	25.78266605500918	22.96433499519138	23.89403215807427	27.35896679172517
38	25.72318701023718	22.907083005840036	24.503048490420028	26.866681493502753
39	25.354556312990066	22.48944193930168	24.656578964113198	27.499422783595058
40	25.74492731315308	22.57311559296335	25.23684052646984	26.445116567413724
41	25.823556004535313	21.941025754456685	25.40789959282761	26.827518648180387
42	24.6825915774279	22.187744104969152	25.99214561488243	27.13751870272052
43	25.567732481860862	21.173176435511444	25.69656839544747	27.56252268718022
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	115.0
2	148.0
3	412.5
4	677.0
5	677.0
6	784.5
7	892.0
8	903.5
9	915.0
10	1404.5
11	1894.0
12	1894.0
13	3722.5
14	5551.0
15	9473.5
16	13396.0
17	10331.5
18	7267.0
19	7267.0
20	9630.0
21	11993.0
22	11483.5
23	10974.0
24	14776.5
25	18579.0
26	18579.0
27	24204.5
28	29830.0
29	37392.5
30	44955.0
31	56981.0
32	69007.0
33	69007.0
34	85942.0
35	102877.0
36	126569.0
37	150261.0
38	182753.5
39	215246.0
40	215246.0
41	253353.0
42	291460.0
43	334045.0
44	376630.0
45	423654.0
46	470678.0
47	470678.0
48	524698.0
49	578718.0
50	613328.5
51	647939.0
52	669623.0
53	691307.0
54	691307.0
55	681551.5
56	671796.0
57	605584.0
58	539372.0
59	501735.0
60	464098.0
61	464098.0
62	413471.5
63	362845.0
64	312546.5
65	262248.0
66	223020.0
67	183792.0
68	183792.0
69	154131.5
70	124471.0
71	106211.5
72	87952.0
73	73009.5
74	58067.0
75	58067.0
76	48654.5
77	39242.0
78	32789.0
79	26336.0
80	21215.0
81	16094.0
82	16094.0
83	12924.5
84	9755.0
85	7963.5
86	6172.0
87	4931.0
88	3690.0
89	3690.0
90	2791.0
91	1892.0
92	1424.0
93	956.0
94	678.0
95	400.0
96	400.0
97	270.5
98	141.0
99	95.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6600644.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.81596764613924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40548722885796	43.270990992802815
2	9.987870549950262	10.750138367399003
3	3.4323106408600763	5.541393552000759
4	1.71628154711017	3.694534088437868
5	0.980268924617207	2.6377060365857656
6	0.664413153904091	2.145362207650313
7	0.4589922219871309	1.7290777397802295
8	0.32064997304512716	1.3804870860105587
9	0.2587900573550496	1.2534333618385682
>10	1.5980056032540422	16.13351320242
>50	0.1281822721196082	4.704281099154778
>100	0.046154357303527335	4.200832625715398
>500	0.0014658989234990413	0.5422193431162081
>1k	9.584351056408179E-4	1.0967691483084503
>5k	1.1275707112748982E-4	0.45418442691430116
>10k+	5.637853556374491E-5	0.4650767218648495
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	18673	0.28289663857041825	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	11984	0.181558041912274	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	8777	0.13297187365354046	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8179	0.123912151602177	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT	6976	0.10568665724132373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.5450110625569266E-5
2	0.0	0.0	0.0	0.0	4.5450110625569266E-5
3	0.0	0.0	0.0	0.0	4.5450110625569266E-5
4	0.0	0.0	0.0	0.0	4.5450110625569266E-5
5	0.0	0.0	0.0	0.0	4.5450110625569266E-5
6	0.0	0.0	0.0	0.0	9.090022125113853E-5
7	0.0	0.0	0.0	0.0	9.090022125113853E-5
8	0.0	0.0	0.0	0.0	9.090022125113853E-5
9	0.0	0.0	0.0	0.0	1.363503318767078E-4
10	3.030007375037951E-5	0.0	3.030007375037951E-5	0.0	1.363503318767078E-4
11	4.5450110625569266E-5	0.0	3.030007375037951E-5	0.0	1.363503318767078E-4
12	7.575018437594876E-5	0.0	3.030007375037951E-5	0.0	1.666504056270873E-4
13	7.575018437594876E-5	0.0	3.030007375037951E-5	0.0	1.969504793774668E-4
14	9.090022125113853E-5	1.5150036875189755E-5	3.030007375037951E-5	1.5150036875189755E-5	3.0300073750379506E-4
15	9.090022125113853E-5	1.5150036875189755E-5	3.030007375037951E-5	3.030007375037951E-5	3.636008850045541E-4
16	1.0605025812632828E-4	1.5150036875189755E-5	3.030007375037951E-5	9.090022125113853E-5	4.3935106938050285E-4
17	1.0605025812632828E-4	1.5150036875189755E-5	3.030007375037951E-5	1.0605025812632828E-4	4.3935106938050285E-4
18	1.0605025812632828E-4	1.5150036875189755E-5	3.030007375037951E-5	1.0605025812632828E-4	4.696511431308824E-4
19	1.0605025812632828E-4	1.5150036875189755E-5	3.030007375037951E-5	1.969504793774668E-4	4.696511431308824E-4
20	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	3.7875092187974384E-4	5.302512906316414E-4
21	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	8.181019912602467E-4	5.605513643820209E-4
22	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.0013938033925174574	5.908514381324005E-4
23	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.0017574042775220114	6.363015487579697E-4
24	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.0024997560844063093	6.363015487579697E-4
25	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.002908807080036433	6.363015487579697E-4
26	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.003832959329423008	6.363015487579697E-4
27	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.007075067220713615	6.363015487579697E-4
28	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.02542176187656841	6.363015487579697E-4
29	1.2120029500151804E-4	1.5150036875189755E-5	3.030007375037951E-5	0.06855391686023364	6.363015487579697E-4
30	1.363503318767078E-4	1.5150036875189755E-5	3.030007375037951E-5	0.13238102221540807	6.363015487579697E-4
31	1.363503318767078E-4	1.5150036875189755E-5	3.030007375037951E-5	0.35060215336564127	6.363015487579697E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2870	0.0	18.95122	37
TCTTATA	4825	0.0	15.643523	37
GTCTTAG	1045	0.0	14.870813	1
TATACCG	305	0.0	14.557377	5
TAGTACT	495	0.0	14.202021	4
CTCTTAT	7985	0.0	13.692549	37
TAGATCG	285	0.0	13.631578	5
TATACTA	275	0.0	13.454546	5
TTAGAAC	695	0.0	13.043165	3
ATAGTAC	525	0.0	13.038095	3
TTACACT	835	0.0	12.628742	4
TAAGACT	955	0.0	12.397905	4
TTAGTAC	330	0.0	12.333333	3
GTATAAG	560	0.0	12.223214	1
TAAGACA	870	0.0	12.12069	4
TAGGACA	1000	0.0	12.025001	4
TTAATAC	585	0.0	12.017094	3
CTAAGAC	865	0.0	11.976879	3
GTATAAT	510	0.0	11.970588	1
TTAGCGC	295	1.6370905E-11	11.915255	4
>>END_MODULE