FastQCFastQC Report
Thu 9 Feb 2017
SRR2727293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174242
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69290.2182883346638347No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT36170.11394846391673981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATATA1900.019.47368436
TACTTAC5050.019.4158431
CTTATAC17350.018.873237
TCTATAC1700.018.53
TATACCG1351.1550583E-917.8148145
CGTTATG2250.016.44444524
CTCGTTA8900.016.42134922
TTACGGG4300.016.34883726
TCGTTAC7700.015.61688223
GTCTTAG3450.015.5507241
ATTACGG6600.015.41666725
ATTATAC1201.9380168E-615.4166673
TACATAT2650.015.35849135
GTGTACT7600.015.33552628
AGTGTAC8700.015.31034627
TCTTATA28950.015.208981537
CATTACG6150.015.0406524
ATCTCGT11900.014.92437120
GTAAGTA6850.014.5839412
TTACATA3050.014.55737734
Produced by FastQC (version 0.11.5)