##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727293.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3174242
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00925323274029	33.0	31.0	34.0	30.0	34.0
2	32.154529805856015	33.0	31.0	34.0	30.0	34.0
3	32.22877713797499	34.0	31.0	34.0	30.0	34.0
4	35.846835559481605	37.0	35.0	37.0	35.0	37.0
5	35.70571777451121	37.0	35.0	37.0	35.0	37.0
6	35.72216107026497	37.0	35.0	37.0	35.0	37.0
7	35.66267757782803	37.0	35.0	37.0	33.0	37.0
8	35.621580522216014	37.0	35.0	37.0	33.0	37.0
9	37.35006341671492	39.0	37.0	39.0	34.0	39.0
10	37.16710635168963	39.0	37.0	39.0	34.0	39.0
11	37.2807788442091	39.0	37.0	39.0	34.0	39.0
12	37.167308604699954	39.0	37.0	39.0	33.0	39.0
13	37.22326999642749	39.0	37.0	39.0	34.0	39.0
14	38.33638676572234	40.0	38.0	41.0	34.0	41.0
15	38.290951981606945	40.0	38.0	41.0	34.0	41.0
16	38.18840025429693	40.0	38.0	41.0	33.0	41.0
17	38.12694715777814	40.0	38.0	41.0	33.0	41.0
18	38.063337010851725	40.0	37.0	41.0	33.0	41.0
19	38.058838929104965	40.0	37.0	41.0	33.0	41.0
20	37.91788055227043	40.0	37.0	41.0	33.0	41.0
21	37.819897474735704	40.0	37.0	41.0	33.0	41.0
22	37.74703125974642	40.0	37.0	41.0	32.0	41.0
23	37.603476987576876	39.0	37.0	41.0	32.0	41.0
24	37.476642297594196	39.0	37.0	41.0	32.0	41.0
25	37.36679969580139	39.0	37.0	41.0	31.0	41.0
26	36.95366137805498	39.0	36.0	40.0	31.0	41.0
27	36.70356324439032	39.0	36.0	40.0	30.0	41.0
28	36.484614279566586	39.0	36.0	40.0	30.0	41.0
29	36.439208478748625	39.0	36.0	40.0	30.0	41.0
30	36.35588653921157	39.0	35.0	40.0	30.0	41.0
31	36.131025296748014	39.0	35.0	40.0	29.0	41.0
32	36.014689491223415	39.0	35.0	40.0	28.0	41.0
33	35.80996849011512	38.0	35.0	40.0	27.0	41.0
34	35.639486214346604	38.0	35.0	40.0	26.0	41.0
35	35.46632014824326	38.0	34.0	40.0	26.0	41.0
36	35.270456694858176	38.0	34.0	40.0	25.0	41.0
37	35.02086513882684	38.0	34.0	40.0	24.0	41.0
38	34.889255765628455	38.0	33.0	40.0	24.0	41.0
39	34.691082784488394	38.0	33.0	40.0	23.0	41.0
40	34.55844450423125	38.0	33.0	40.0	23.0	41.0
41	34.39328412893535	38.0	33.0	40.0	23.0	41.0
42	34.18436117977142	38.0	33.0	40.0	22.0	41.0
43	30.514096593769473	34.0	26.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	11.0
11	20.0
12	13.0
13	16.0
14	33.0
15	80.0
16	164.0
17	290.0
18	585.0
19	1213.0
20	2428.0
21	4285.0
22	7344.0
23	11376.0
24	16663.0
25	23394.0
26	31037.0
27	39594.0
28	50013.0
29	62442.0
30	76837.0
31	94844.0
32	115208.0
33	142318.0
34	180954.0
35	232589.0
36	317151.0
37	479885.0
38	809321.0
39	474133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1778541144626	15.746404968493266	11.533336147653518	28.54240476939061
2	20.34986620427806	19.314248882095317	32.20954798027372	28.126336933352906
3	19.77023175926725	19.936759705151655	29.372555715663772	30.920452819917323
4	16.42867809070638	14.001452945301587	30.211401651165854	39.35846731282618
5	16.09697685305657	35.3572285918969	32.608162830685245	15.937631724361282
6	40.165872671333815	31.68624194374594	12.574750129322213	15.57313525559803
7	32.54966067489498	29.44076097537617	18.042165657186818	19.96741269254203
8	30.641173546314366	32.9128339931234	17.35412737907192	19.091865081490322
9	29.003900773791035	12.286807370074493	15.501779637469355	43.207512218665116
10	16.90324808253435	25.870711810882725	31.594030952901512	25.632009153681413
11	40.26473091843659	19.038119966908635	18.483719892812207	22.213429221842567
12	23.636162586217434	23.028994008648365	26.99091625654251	26.343927148591696
13	34.70318268109363	17.237312088996365	21.246080166540548	26.81342506336946
14	24.228429968477514	18.10123487749201	22.953385406657713	34.71694974737276
15	29.236775267922233	25.0456959488281	19.339357238673045	26.378171544576627
16	27.52395690057658	23.65122129944724	21.799440622359604	27.025381177616577
17	27.133690499968182	23.389174486381314	22.255423499531542	27.22171151411896
18	26.939344889268053	23.385362552697618	23.103720510282454	26.571572047751875
19	28.74481529763641	23.07615487414003	22.489463626276763	25.689566201946796
20	27.400651872163493	22.37627124837993	22.614879394828748	27.608197484627826
21	27.098091449864253	23.40253200606633	22.922102347584083	26.577274196485334
22	28.44524771583263	22.331095108690516	21.420578519218132	27.803078656258723
23	27.261217008659077	22.604168176213406	22.45364405108369	27.680970764043828
24	26.8311615812531	22.241687936836573	22.629623072216926	28.2975274096934
25	27.708032342839644	21.587988565459092	22.19638578281051	28.507593308890755
26	27.19420888514486	22.11567360018549	23.10034962677704	27.58976788789261
27	28.63527733550246	22.368080316497608	22.331347137363817	26.665295210636113
28	27.26767524341244	23.45186661886523	21.767590498771046	27.51286763895128
29	27.293823218267544	23.10665034360959	22.672247421589155	26.92727901653371
30	28.17456892070611	22.53142640038157	22.20183590287067	27.092168776041653
31	27.438046626564706	22.274514671534178	22.536592988184264	27.75084571371685
32	26.889033665360106	22.459629732074617	22.458716128133897	28.192620474431372
33	27.213678100157455	22.184603442333632	22.60158488231206	28.000133575196852
34	27.647545461247127	22.303340451043116	23.211903818297408	26.837210269412353
35	27.308314866982414	22.227794856220793	22.95058158766723	27.513308689129563
36	26.72518352412954	21.659501701508578	23.850197937019292	27.765116837342585
37	26.272193487453066	21.746987154728593	24.172448099420272	27.80837125839807
38	26.913763978927886	21.139377526981246	24.03345428609413	27.913404207996745
39	25.670317512023345	21.37915130604409	25.105741780242337	27.844789401690228
40	26.867170177951145	20.628515406197764	25.219028668891657	27.28528574695943
41	25.805121348655835	21.036770353363103	25.575208191435937	27.58290010654512
42	25.09846445230074	20.904864846473583	26.24424980830069	27.752420892924988
43	25.12839285725537	19.938933452458887	25.69586691877935	29.236806771506394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	68.5
2	84.0
3	275.5
4	467.0
5	467.0
6	548.5
7	630.0
8	587.0
9	544.0
10	761.0
11	978.0
12	978.0
13	2107.5
14	3237.0
15	6295.5
16	9354.0
17	6549.0
18	3744.0
19	3744.0
20	4397.0
21	5050.0
22	3965.0
23	2880.0
24	3827.0
25	4774.0
26	4774.0
27	6159.0
28	7544.0
29	9589.5
30	11635.0
31	14735.5
32	17836.0
33	17836.0
34	22815.5
35	27795.0
36	35813.0
37	43831.0
38	56131.5
39	68432.0
40	68432.0
41	84072.5
42	99713.0
43	121010.0
44	142307.0
45	171281.0
46	200255.0
47	200255.0
48	235538.5
49	270822.0
50	300919.5
51	331017.0
52	346293.0
53	361569.0
54	361569.0
55	342477.5
56	323386.0
57	305858.5
58	288331.0
59	274601.0
60	260871.0
61	260871.0
62	233397.0
63	205923.0
64	177271.0
65	148619.0
66	127049.0
67	105479.0
68	105479.0
69	88674.5
70	71870.0
71	61616.5
72	51363.0
73	43504.0
74	35645.0
75	35645.0
76	30224.5
77	24804.0
78	20887.0
79	16970.0
80	13792.5
81	10615.0
82	10615.0
83	8589.5
84	6564.0
85	5288.0
86	4012.0
87	3401.5
88	2791.0
89	2791.0
90	2104.0
91	1417.0
92	1039.0
93	661.0
94	457.5
95	254.0
96	254.0
97	169.5
98	85.0
99	58.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3174242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.0231602341951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.31749469745027	50.00979334536759
2	8.941349765325036	10.733761393481748
3	2.8580069614077566	5.146398293850686
4	1.4217479417830754	3.4135121808913054
5	0.8040741935975171	2.4131537081242493
6	0.5240690582790525	1.8873768635320378
7	0.3677642567110586	1.5452061036284273
8	0.2665215417826999	1.2797972166630174
9	0.21136792390622325	1.1418273688493157
>10	1.1756873663254084	12.716422831875681
>50	0.07083530179941648	2.912558499127053
>100	0.03662112120947699	4.026992614095488
>500	0.0031481438294450586	1.2592397122296233
>1k	0.0012592575298359515	1.2957407817607727
>5k	5.2469063743164645E-5	0.2182190865231119
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6929	0.2182883346638347	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	3617	0.11394846391673981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.15035841627702E-5	0.0	0.0	0.0
6	0.0	3.15035841627702E-5	0.0	0.0	3.15035841627702E-5
7	0.0	3.15035841627702E-5	0.0	0.0	9.45107524883106E-5
8	0.0	3.15035841627702E-5	0.0	0.0	9.45107524883106E-5
9	0.0	3.15035841627702E-5	0.0	0.0	2.520286733021616E-4
10	3.15035841627702E-5	3.15035841627702E-5	0.0	3.15035841627702E-5	2.520286733021616E-4
11	6.30071683255404E-5	3.15035841627702E-5	0.0	3.15035841627702E-5	2.520286733021616E-4
12	9.45107524883106E-5	3.15035841627702E-5	0.0	6.30071683255404E-5	2.520286733021616E-4
13	9.45107524883106E-5	3.15035841627702E-5	0.0	6.30071683255404E-5	2.520286733021616E-4
14	1.890215049766212E-4	3.15035841627702E-5	0.0	6.30071683255404E-5	3.780430099532424E-4
15	2.2052508913939138E-4	3.15035841627702E-5	0.0	1.5751792081385098E-4	3.780430099532424E-4
16	2.2052508913939138E-4	3.15035841627702E-5	0.0	3.1503584162770196E-4	5.985680990926338E-4
17	2.2052508913939138E-4	3.15035841627702E-5	0.0	5.985680990926338E-4	5.985680990926338E-4
18	2.2052508913939138E-4	3.15035841627702E-5	0.0	6.300716832554039E-4	6.930788515809444E-4
19	3.465394257904722E-4	3.15035841627702E-5	0.0	9.136039407203358E-4	7.245824357437145E-4
20	3.465394257904722E-4	3.15035841627702E-5	0.0	0.0013231505348363483	7.245824357437145E-4
21	3.465394257904722E-4	3.15035841627702E-5	0.0	0.002488783148858846	7.87589604069255E-4
22	3.780430099532424E-4	3.15035841627702E-5	0.0	0.004505012535276139	7.87589604069255E-4
23	3.780430099532424E-4	3.15035841627702E-5	0.0	0.005733652317624176	9.136039407203358E-4
24	3.780430099532424E-4	6.30071683255404E-5	0.0	0.00787589604069255	9.45107524883106E-4
25	3.780430099532424E-4	9.45107524883106E-5	0.0	0.009388068080505518	9.45107524883106E-4
26	3.780430099532424E-4	9.45107524883106E-5	0.0	0.011561815387736664	9.766111090458761E-4
27	4.0954659411601257E-4	9.45107524883106E-5	0.0	0.016318856596314962	0.0010081146932086464
28	4.0954659411601257E-4	9.45107524883106E-5	0.0	0.042372320698925915	0.0010081146932086464
29	4.0954659411601257E-4	9.45107524883106E-5	0.0	0.10944345138146366	0.0010081146932086464
30	4.0954659411601257E-4	9.45107524883106E-5	0.0	0.20474179347384353	0.0010081146932086464
31	4.0954659411601257E-4	9.45107524883106E-5	0.0	0.49551987529621244	0.0010081146932086464
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATATA	190	0.0	19.473684	36
TACTTAC	505	0.0	19.41584	31
CTTATAC	1735	0.0	18.8732	37
TCTATAC	170	0.0	18.5	3
TATACCG	135	1.1550583E-9	17.814814	5
CGTTATG	225	0.0	16.444445	24
CTCGTTA	890	0.0	16.421349	22
TTACGGG	430	0.0	16.348837	26
TCGTTAC	770	0.0	15.616882	23
GTCTTAG	345	0.0	15.550724	1
ATTACGG	660	0.0	15.416667	25
ATTATAC	120	1.9380168E-6	15.416667	3
TACATAT	265	0.0	15.358491	35
GTGTACT	760	0.0	15.335526	28
AGTGTAC	870	0.0	15.310346	27
TCTTATA	2895	0.0	15.2089815	37
CATTACG	615	0.0	15.04065	24
ATCTCGT	1190	0.0	14.924371	20
GTAAGTA	685	0.0	14.583941	2
TTACATA	305	0.0	14.557377	34
>>END_MODULE