FastQCFastQC Report
Thu 9 Feb 2017
SRR2727292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485088
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT206041.3873925316210218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76230.5133029153827922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67640.45546122519338916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53470.3600460040078433No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA26180.17628584972742356No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT26040.1753431446486673No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.1457152707448986No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.11339395375896916No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.1063236656682971No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT15160.10208149281389386No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG14900.10033075481048935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGA656.903867E-519.92307710
GTACCGT801.6171884E-518.56
TAAACCG500.007035826318.55
TGTACGC609.238549E-418.52
TATACCG752.0677783E-417.2666665
CTTATAC15600.016.60256437
TAGTACT1708.54925E-1116.3235284
TCGGACG803.383397E-416.187513
TCTATAC1151.2427154E-616.0869563
CGACCGA700.002592972215.85714218
TTATACT2750.015.4727274
CGCCTTA1802.0190782E-1015.41666625
TACACCG855.365463E-415.2352935
TATACAG2800.015.1964285
ACCGTCC1101.4523572E-515.1363648
GTATTAG2109.094947E-1214.97619151
CCGATTT750.00410515614.821
CGCTACG750.00410515614.810
CCGACCG1001.09370536E-414.89
CCCTATA1252.9599687E-614.7999992
Produced by FastQC (version 0.11.5)