##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727291.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7613328
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10846623185025	33.0	31.0	34.0	30.0	34.0
2	32.24702390334424	33.0	31.0	34.0	30.0	34.0
3	32.3275236532565	34.0	31.0	34.0	30.0	34.0
4	35.91515720851643	37.0	35.0	37.0	35.0	37.0
5	35.783577168880676	37.0	35.0	37.0	35.0	37.0
6	35.8066683847064	37.0	35.0	37.0	35.0	37.0
7	35.7344735442897	37.0	35.0	37.0	35.0	37.0
8	35.700931051440314	37.0	35.0	37.0	33.0	37.0
9	37.437735639394496	39.0	37.0	39.0	35.0	39.0
10	37.27858802352927	39.0	37.0	39.0	34.0	39.0
11	37.38491090886929	39.0	37.0	39.0	34.0	39.0
12	37.280365038784616	39.0	37.0	39.0	34.0	39.0
13	37.332538936979994	39.0	37.0	39.0	34.0	39.0
14	38.464630316728766	40.0	38.0	41.0	34.0	41.0
15	38.42875192031658	40.0	38.0	41.0	34.0	41.0
16	38.336228387900796	40.0	38.0	41.0	34.0	41.0
17	38.282802737515055	40.0	38.0	41.0	33.0	41.0
18	38.235354499372676	40.0	38.0	41.0	33.0	41.0
19	38.232804497586336	40.0	38.0	41.0	34.0	41.0
20	38.11041413163862	40.0	38.0	41.0	33.0	41.0
21	38.018159732511194	40.0	37.0	41.0	33.0	41.0
22	37.94163577347515	40.0	37.0	41.0	33.0	41.0
23	37.80770262360954	40.0	37.0	41.0	33.0	41.0
24	37.68761348519333	40.0	37.0	41.0	32.0	41.0
25	37.59472204533944	40.0	37.0	41.0	32.0	41.0
26	37.20149624448073	39.0	37.0	41.0	31.0	41.0
27	36.97031744855863	39.0	36.0	40.0	30.0	41.0
28	36.77683228674766	39.0	36.0	40.0	30.0	41.0
29	36.729143812009674	39.0	36.0	40.0	30.0	41.0
30	36.636666514302284	39.0	36.0	40.0	30.0	41.0
31	36.421305899338634	39.0	35.0	40.0	30.0	41.0
32	36.29051119300259	39.0	35.0	40.0	30.0	41.0
33	36.10096162939519	39.0	35.0	40.0	28.0	41.0
34	35.93905648095025	38.0	35.0	40.0	27.0	41.0
35	35.77001187916769	38.0	35.0	40.0	27.0	41.0
36	35.58600168021133	38.0	35.0	40.0	26.0	41.0
37	35.34261508239235	38.0	34.0	40.0	25.0	41.0
38	35.21248591942972	38.0	34.0	40.0	25.0	41.0
39	35.03463111007433	38.0	34.0	40.0	24.0	41.0
40	34.88896866127402	38.0	33.0	40.0	24.0	41.0
41	34.72306421055286	38.0	33.0	40.0	23.0	41.0
42	34.50082263630307	38.0	33.0	40.0	23.0	41.0
43	30.85999066374127	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	34.0
11	51.0
12	58.0
13	51.0
14	86.0
15	172.0
16	342.0
17	628.0
18	1218.0
19	2445.0
20	4716.0
21	8862.0
22	14953.0
23	23651.0
24	35420.0
25	48906.0
26	65798.0
27	85257.0
28	109009.0
29	136652.0
30	169040.0
31	209692.0
32	258822.0
33	322154.0
34	410910.0
35	535012.0
36	742021.0
37	1143675.0
38	1978172.0
39	1305513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95868666107647	16.54360616014442	12.036877959284034	27.460829219495075
2	19.49323607231949	20.14682672282082	33.01352575378336	27.346411451076325
3	19.497662520253954	20.734073193746546	29.603663470167053	30.164600815832443
4	15.62805385502897	14.350885709902425	31.19602885886435	38.82503157620425
5	15.646968053918076	35.90125106917763	32.97626215499976	15.475518721904532
6	39.054734013824174	32.82245294042237	12.812241899994326	15.310571145759122
7	31.932224646041785	29.57551546445917	18.626020053253978	19.86623983624507
8	30.152293451694185	33.48318895494848	17.67286264298609	18.691654950371245
9	28.122904989775826	12.852946832186923	16.1163685578764	42.90777962016086
10	16.586044893901853	26.467124495358668	32.010429604504104	24.936401006235382
11	39.35862214264248	19.617018996160418	19.02425588389204	22.000102977305065
12	23.14974476339388	23.85691250922067	27.759056223506988	25.23428650387846
13	34.21386810078326	17.629727761630658	21.885908501512084	26.270495636074
14	23.798370436686824	18.45937808012475	23.94047649070157	33.80177499248686
15	28.283399848266093	25.695241292638382	20.03663837943144	25.984720479664087
16	26.94297421574376	23.935642862096575	22.391088365035632	26.730294557124033
17	26.357921266494756	24.27255202980878	23.390506753419793	25.979019950276673
18	26.37125314973951	23.40553303364836	24.201820806879727	26.021393009732407
19	27.52240544476739	23.805371317247857	23.497122414796788	25.175100823187968
20	26.8035476732383	23.162695735688782	23.535147835480092	26.498608755592823
21	26.352128793084965	24.07524015778645	23.398978738338872	26.17365231078971
22	27.342812499343257	23.289736104893944	22.469109435453195	26.8983419603096
23	26.52109563649432	23.169407649322345	23.10079113890798	27.20870557527536
24	26.343013200009246	23.05322980961808	23.244814882532317	27.358942107840356
25	26.90676140578733	22.534218938156876	23.022848877652454	27.536170778403346
26	26.4796551521227	22.88667978051123	23.826203205746555	26.80746186161952
27	27.50774694062833	23.179377008320145	23.304328934731302	26.008547116320223
28	26.706953910300463	23.757718043935583	23.059258710513983	26.476069335249974
29	26.43230398059824	23.662253878986956	23.63872934411863	26.266712796296183
30	26.721848841925635	23.51783608955243	23.359666101342277	26.400648967179663
31	26.42641956316607	22.930090493933797	23.39400062627014	27.249489316629994
32	25.88879659460357	23.32361090970992	23.48679841456982	27.300794081116692
33	26.461818011781446	23.049827880790108	23.380143348611803	27.108210758816643
34	26.679291894425145	23.067940853198497	23.897814464318365	26.354952788057993
35	26.363332828954693	23.113742111202882	23.966733076520544	26.55619198332188
36	25.931051440316246	22.656688375963835	24.45352413556857	26.958736048151348
37	25.43815267121028	22.45206038673232	24.721619769961308	27.388167172096097
38	25.696397160348273	22.191110116364353	25.024155008164627	27.08833771512274
39	25.095070119138434	22.032966397874883	25.657110793072356	27.21485268991432
40	25.7336344894112	21.717073006706133	26.0483851477304	26.500907356152265
41	25.078704083155223	21.707405749496147	26.368875740018034	26.8450144273306
42	24.343913726034135	21.762401409738292	26.892562621760156	27.001122242467417
43	24.51373433536556	20.74582889375054	26.625544045915266	28.114892724968634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	231.0
2	347.0
3	1235.0
4	2123.0
5	2123.0
6	2484.5
7	2846.0
8	2772.0
9	2698.0
10	3796.5
11	4895.0
12	4895.0
13	10999.0
14	17103.0
15	33589.5
16	50076.0
17	34519.5
18	18963.0
19	18963.0
20	21745.0
21	24527.0
22	18694.5
23	12862.0
24	16427.0
25	19992.0
26	19992.0
27	26017.0
28	32042.0
29	39449.0
30	46856.0
31	57720.5
32	68585.0
33	68585.0
34	84978.5
35	101372.0
36	126551.0
37	151730.0
38	185507.5
39	219285.0
40	219285.0
41	260552.0
42	301819.0
43	349955.0
44	398091.0
45	457585.0
46	517079.0
47	517079.0
48	585904.5
49	654730.0
50	703451.5
51	752173.0
52	783908.5
53	815644.0
54	815644.0
55	771225.0
56	726806.0
57	677901.0
58	628996.0
59	592965.0
60	556934.0
61	556934.0
62	499338.5
63	441743.0
64	382306.0
65	322869.0
66	276630.0
67	230391.0
68	230391.0
69	193510.5
70	156630.0
71	135122.5
72	113615.0
73	95148.0
74	76681.0
75	76681.0
76	64706.0
77	52731.0
78	44450.0
79	36169.0
80	28982.0
81	21795.0
82	21795.0
83	17592.0
84	13389.0
85	10773.0
86	8157.0
87	6799.0
88	5441.0
89	5441.0
90	4091.5
91	2742.0
92	2036.0
93	1330.0
94	984.5
95	639.0
96	639.0
97	425.0
98	211.0
99	158.5
100	106.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7613328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.89635139518387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9264324853915	42.807130099058924
2	9.744268182001479	10.308724676881194
3	3.3762489063296064	5.357737456404483
4	1.6565003143114154	3.504912908481966
5	0.9774654476758879	2.585217789845724
6	0.6295274610164011	1.9979823474504883
7	0.443656983113715	1.642748497439716
8	0.3123482196633516	1.321766494797818
9	0.24161609441192153	1.150254884947043
>10	1.5076938501457526	14.998469593335084
>50	0.12069838218403726	4.391404387611285
>100	0.057753445759575056	5.470077404376418
>500	0.003678097162108442	1.292096757745027
>1k	0.001863644858457727	1.7167511985093618
>5k	1.7394018233186183E-4	0.6897314754416969
>10k+	7.454579242794077E-5	0.7649940276737914
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36998	0.48596356284662895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10666	0.14009642038278136	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	10537	0.13840202339896562	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9910	0.13016646596600068	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8359	0.1097942975791927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.313486033965698E-5	0.0	0.0	0.0	2.626972067931396E-5
7	2.626972067931396E-5	0.0	1.313486033965698E-5	0.0	2.626972067931396E-5
8	5.253944135862792E-5	0.0	1.313486033965698E-5	0.0	2.626972067931396E-5
9	5.253944135862792E-5	0.0	1.313486033965698E-5	0.0	9.194402237759886E-5
10	1.7075318441554075E-4	0.0	1.313486033965698E-5	0.0	9.194402237759886E-5
11	1.8388804475519773E-4	0.0	1.313486033965698E-5	1.313486033965698E-5	9.194402237759886E-5
12	1.8388804475519773E-4	0.0	1.313486033965698E-5	2.626972067931396E-5	9.194402237759886E-5
13	1.970229050948547E-4	0.0	1.313486033965698E-5	7.880916203794189E-5	9.194402237759886E-5
14	2.2329262577416866E-4	0.0	1.313486033965698E-5	9.194402237759886E-5	1.970229050948547E-4
15	2.3642748611382564E-4	0.0	1.313486033965698E-5	1.7075318441554075E-4	2.1015776543451169E-4
16	2.4956234645348265E-4	0.0	1.313486033965698E-5	3.940458101897094E-4	2.889669274724536E-4
17	2.4956234645348265E-4	0.0	1.313486033965698E-5	8.931705030966747E-4	3.0210178781211056E-4
18	2.4956234645348265E-4	0.0	1.313486033965698E-5	0.0011164631288708433	3.283715084914245E-4
19	2.889669274724536E-4	0.0	1.313486033965698E-5	0.0018651501682312912	3.283715084914245E-4
20	3.1523664815176754E-4	0.0	1.313486033965698E-5	0.0028502646937055647	3.415063688310815E-4
21	3.6777608951039546E-4	0.0	1.313486033965698E-5	0.005004381789409309	3.546412291707385E-4
22	4.071806705293664E-4	0.0	1.313486033965698E-5	0.008445715198399439	3.546412291707385E-4
23	4.071806705293664E-4	0.0	1.313486033965698E-5	0.010652371735461812	4.597201118879943E-4
24	4.071806705293664E-4	0.0	1.313486033965698E-5	0.015026280228567586	4.597201118879943E-4
25	4.071806705293664E-4	1.313486033965698E-5	1.313486033965698E-5	0.018191781570424916	4.597201118879943E-4
26	4.071806705293664E-4	1.313486033965698E-5	1.313486033965698E-5	0.022040295649944412	4.728549722276513E-4
27	4.071806705293664E-4	1.313486033965698E-5	1.313486033965698E-5	0.028003522244148683	4.728549722276513E-4
28	4.071806705293664E-4	1.313486033965698E-5	1.313486033965698E-5	0.05737306996362169	4.728549722276513E-4
29	4.071806705293664E-4	1.313486033965698E-5	1.313486033965698E-5	0.12961480183173507	4.728549722276513E-4
30	4.071806705293664E-4	1.313486033965698E-5	1.313486033965698E-5	0.2359283614209187	4.728549722276513E-4
31	4.071806705293664E-4	1.313486033965698E-5	1.313486033965698E-5	0.5494180731475118	4.8598983256730827E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4705	0.0	18.087141	37
TTAATAC	525	0.0	16.914286	3
TAATACC	435	0.0	15.310346	4
TCTTATA	7585	0.0	14.707315	37
ATACTAG	315	0.0	14.68254	1
TATACCG	305	0.0	14.557377	5
GAGTACT	9920	0.0	14.397178	11
AGTACTT	10030	0.0	14.239282	12
TCTATAC	520	0.0	14.230769	3
GTCTTAG	1185	0.0	13.894514	1
GTACTTT	10325	0.0	13.760775	13
TACTTTT	10305	0.0	13.7336235	14
TCGTTAC	1230	0.0	13.536586	23
GTATAAG	590	0.0	13.483052	1
TAGATCG	270	3.6379788E-12	13.0185175	5
CTCTTAT	12100	0.0	12.995869	37
CGTTACG	1125	0.0	12.826667	24
ACTTTTT	11190	0.0	12.779715	15
ATTACGG	870	0.0	12.758621	25
TACTTAC	1045	0.0	12.569378	31
>>END_MODULE