FastQCFastQC Report
Thu 9 Feb 2017
SRR2727290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6697950
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT205840.30731791070402137No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT89910.13423510178487447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC45100.019.73059837
TATACCG2950.015.0508475
TCTTATA76850.014.92517937
TAATACG1508.119787E-814.84
TATACTG6250.014.5045
TACTTAC8950.014.469274531
TCGTTAC7900.014.2848123
CTCGTTA10150.014.03448322
TGTTACG8100.013.93209824
GAGTACT59200.013.812511
TAGATCG3650.013.6849325
CGTTACG7250.013.52413824
GTTACGG15750.013.27301725
GTATTAA6150.013.2357721
AGTACTT62850.013.09864812
TAGACTG8500.013.0588235
TTATACT6850.012.9635044
CTATACT5600.012.8839284
TTTATAC5750.012.8695653
GTCTTAG10250.012.8146331
Produced by FastQC (version 0.11.5)