FastQCFastQC Report
Thu 9 Feb 2017
SRR2727289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5287816
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT283960.5370080955918285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82030.15513020876671957No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78740.14890835838463365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66290.12536366620926295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC36750.019.43129337
TACTTAC9950.017.84924731
TCGTTAC10250.017.32682823
GTACTTA11200.015.85714230
CTCGTTA12300.015.64227622
AGTGTAC13600.014.96323627
CGTTACG9450.014.87830724
GAGTACT74550.014.81488911
AGTACTT77100.014.324903512
GTTACGG18750.013.81333325
GTACTTT79550.013.79069813
TACTTTT80200.013.77119614
TCTTATA59950.013.70141737
TACGGAT23050.013.56399227
TAATACT5200.013.5192314
GGGTAAG40150.013.2702361
ATAATAC4200.013.2142873
CCGATTT10800.013.18981421
GTAAGAG42100.013.18289853
TTACATA6600.013.17424334
Produced by FastQC (version 0.11.5)