##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5287816
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.102932098998906	33.0	31.0	34.0	30.0	34.0
2	32.24289650018079	33.0	31.0	34.0	30.0	34.0
3	32.320908859158486	34.0	31.0	34.0	30.0	34.0
4	35.91258659529757	37.0	35.0	37.0	35.0	37.0
5	35.78526994887871	37.0	35.0	37.0	35.0	37.0
6	35.80646244120446	37.0	35.0	37.0	35.0	37.0
7	35.74389729143374	37.0	35.0	37.0	35.0	37.0
8	35.71288694614185	37.0	35.0	37.0	33.0	37.0
9	37.44616529773351	39.0	37.0	39.0	35.0	39.0
10	37.2830544784463	39.0	37.0	39.0	34.0	39.0
11	37.394286034158526	39.0	37.0	39.0	34.0	39.0
12	37.289996664029154	39.0	37.0	39.0	34.0	39.0
13	37.347446469393034	39.0	37.0	39.0	34.0	39.0
14	38.47130384264506	40.0	38.0	41.0	34.0	41.0
15	38.43931275218351	40.0	38.0	41.0	34.0	41.0
16	38.35110242111299	40.0	38.0	41.0	34.0	41.0
17	38.306606167839426	40.0	38.0	41.0	34.0	41.0
18	38.25728712950678	40.0	38.0	41.0	33.0	41.0
19	38.26362301562687	40.0	38.0	41.0	34.0	41.0
20	38.14136271004891	40.0	38.0	41.0	33.0	41.0
21	38.05212454442439	40.0	38.0	41.0	33.0	41.0
22	37.985010257543	40.0	37.0	41.0	33.0	41.0
23	37.85635600784899	40.0	37.0	41.0	33.0	41.0
24	37.73740727740905	40.0	37.0	41.0	32.0	41.0
25	37.64325195884275	40.0	37.0	41.0	32.0	41.0
26	37.2587682703029	39.0	37.0	41.0	31.0	41.0
27	37.038931195790475	39.0	36.0	41.0	31.0	41.0
28	36.8356869830569	39.0	36.0	40.0	30.0	41.0
29	36.79014530762795	39.0	36.0	40.0	30.0	41.0
30	36.70528550917808	39.0	36.0	40.0	30.0	41.0
31	36.48847841906753	39.0	36.0	40.0	30.0	41.0
32	36.360994028536545	39.0	35.0	40.0	30.0	41.0
33	36.17350546993315	39.0	35.0	40.0	29.0	41.0
34	36.01334029020677	39.0	35.0	40.0	28.0	41.0
35	35.84307358652419	38.0	35.0	40.0	27.0	41.0
36	35.662183215149696	38.0	35.0	40.0	26.0	41.0
37	35.42331408657185	38.0	34.0	40.0	26.0	41.0
38	35.29643032208382	38.0	34.0	40.0	25.0	41.0
39	35.11203037322025	38.0	34.0	40.0	24.0	41.0
40	34.97621475482505	38.0	34.0	40.0	24.0	41.0
41	34.806870927430154	38.0	33.0	40.0	24.0	41.0
42	34.589456024944894	38.0	33.0	40.0	23.0	41.0
43	31.006659271048765	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	23.0
11	36.0
12	35.0
13	40.0
14	66.0
15	127.0
16	190.0
17	415.0
18	805.0
19	1693.0
20	3194.0
21	5817.0
22	9785.0
23	15546.0
24	23103.0
25	32698.0
26	43908.0
27	57561.0
28	73654.0
29	93174.0
30	115186.0
31	143141.0
32	177866.0
33	221747.0
34	283189.0
35	369816.0
36	514496.0
37	797338.0
38	1387968.0
39	915192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39867347880486	17.188003516007363	12.365747976102043	27.04757502908573
2	19.40273640383856	20.73402327161157	33.405492929406016	26.457747395143855
3	19.095331607605107	21.25075834711344	30.329175599150954	29.324734446130503
4	15.400479139213619	14.724056207704656	31.83036247857338	38.045102174508344
5	15.22588910052846	36.653620322643604	33.131542398600864	14.98894817822708
6	38.073677298907526	33.717984892061295	13.11615230181988	15.092185507211294
7	31.33639672787404	30.4508137196907	18.930102711592085	19.282686840843176
8	29.77219706585857	34.13721279257826	17.739554477689843	18.351035663873326
9	28.421412545368447	12.922802154991778	16.403709962676462	42.252075336963316
10	16.25542568046997	27.076660761267036	32.19663846094493	24.47127509731806
11	39.027190053511696	20.14890457610477	19.332121238711785	21.491784131671753
12	22.75812925411928	24.314594153805654	28.045075698549272	24.8822008935258
13	33.67367548341319	18.17215273753852	22.28422849811718	25.869943280931107
14	23.382716040043753	19.104560370481877	24.296212273649463	33.21651131582491
15	27.782888058132126	26.56467244699891	20.322586867621716	25.32985262724724
16	26.18307823116387	24.828530342205553	22.696515915077224	26.291875511553354
17	26.01436963767272	24.676331400336167	23.48338141871805	25.825917543273064
18	25.794997405356014	24.293035158560734	24.423334699997127	25.488632736086124
19	27.471814450427175	24.20159854276321	23.81728108542355	24.50930592138607
20	26.199493325788946	23.700257346322186	23.981564411469687	26.118684916419177
21	25.848894893468305	24.55340730464146	24.230646452145837	25.367051349744397
22	26.967976949273577	23.641764388170845	22.930903798468023	26.459354864087555
23	25.969190304655076	23.804742827662686	23.76792233315229	26.45814453452995
24	25.669520270750724	23.559915851837506	23.882582147336443	26.887981730075328
25	26.288471459672575	23.06708478509842	23.68253736514281	26.96190639008619
26	25.85216656555372	23.53069773986084	24.35714480231536	26.25999089227008
27	27.015690409802456	23.89595250666816	23.68155397237725	25.406803111152126
28	25.989557881741725	24.570011513259917	23.292338462609138	26.148092142389224
29	25.892296554948206	24.478934214049808	23.966492026197585	25.6622772048044
30	26.510131971309136	24.07267953347847	23.571584185228836	25.845604309983557
31	25.949295512551874	23.585332772547304	23.91210284170251	26.55326887319831
32	25.367051349744397	24.003823128490097	23.860285607517355	26.76883991424815
33	25.822418934395596	23.569579576899045	23.8465937543969	26.761407734308456
34	26.1282919072827	23.62508453395504	24.586388785086317	25.66023477367594
35	26.04545997818381	23.663361206214436	24.18119314287789	26.109985672723862
36	25.474676123374945	23.07523559821295	24.974810016082255	26.475278262329855
37	25.002628684507933	23.119185690273643	25.212488482957802	26.665697142260626
38	25.434546133980458	22.601675247398926	25.379173556719824	26.58460506190079
39	24.539450691930277	22.710264502395695	26.22180877700737	26.528476028666653
40	25.39212786526611	22.187912741290543	26.40965570662822	26.010303686815124
41	24.518251013272778	22.276966520771524	26.833081937798138	26.37170052815756
42	23.950398425361247	22.428976348647534	27.155105245719596	26.46551998027163
43	23.98018387931804	21.437716440965417	26.785860173652033	27.796239506064506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	270.5
2	346.0
3	998.0
4	1650.0
5	1650.0
6	1936.0
7	2222.0
8	2196.5
9	2171.0
10	3154.5
11	4138.0
12	4138.0
13	9274.0
14	14410.0
15	27034.5
16	39659.0
17	27901.5
18	16144.0
19	16144.0
20	18565.0
21	20986.0
22	16660.5
23	12335.0
24	15808.0
25	19281.0
26	19281.0
27	24844.5
28	30408.0
29	36784.0
30	43160.0
31	52411.5
32	61663.0
33	61663.0
34	74694.0
35	87725.0
36	106383.0
37	125041.0
38	150318.5
39	175596.0
40	175596.0
41	202809.5
42	230023.0
43	262221.5
44	294420.0
45	335904.0
46	377388.0
47	377388.0
48	423600.0
49	469812.0
50	499492.5
51	529173.0
52	537931.5
53	546690.0
54	546690.0
55	514896.5
56	483103.0
57	451101.5
58	419100.0
59	394194.5
60	369289.0
61	369289.0
62	325684.5
63	282080.0
64	240875.5
65	199671.0
66	169659.5
67	139648.0
68	139648.0
69	116367.5
70	93087.0
71	79576.5
72	66066.0
73	55400.0
74	44734.0
75	44734.0
76	37769.5
77	30805.0
78	26063.5
79	21322.0
80	17249.0
81	13176.0
82	13176.0
83	10874.5
84	8573.0
85	6895.5
86	5218.0
87	4546.5
88	3875.0
89	3875.0
90	2923.5
91	1972.0
92	1410.0
93	848.0
94	615.5
95	383.0
96	383.0
97	273.5
98	164.0
99	115.0
100	66.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5287816.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.44297324667836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.44284920411604	44.339908603529494
2	9.591647616630764	10.4439562916759
3	3.29846963894045	5.387354829234472
4	1.5391836630955547	3.3519093996654274
5	0.9050323631449376	2.4636326367038963
6	0.6065613633684247	1.9813802447001538
7	0.4375496602030334	1.6675053111168867
8	0.32888751857973175	1.4324491500162213
9	0.23712304876550658	1.1618715420100154
>10	1.449996292500231	14.778411965643897
>50	0.10644736705884318	3.94773666567143
>100	0.05066139019737723	5.126147022911227
>500	0.0037504029340232068	1.3938512519201993
>1k	0.0017015670337630628	1.5577614307383632
>5k	1.0417757349466772E-4	0.429274856135271
>10k+	3.472585783155591E-5	0.5368487983271891
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28396	0.5370080955918285	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8203	0.15513020876671957	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7874	0.14890835838463365	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6629	0.12536366620926295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.673419801294145E-5
2	1.891139933764715E-5	0.0	0.0	0.0	5.673419801294145E-5
3	1.891139933764715E-5	0.0	0.0	0.0	5.673419801294145E-5
4	1.891139933764715E-5	0.0	0.0	0.0	7.56455973505886E-5
5	1.891139933764715E-5	0.0	0.0	0.0	7.56455973505886E-5
6	3.78227986752943E-5	0.0	0.0	0.0	1.134683960258829E-4
7	7.56455973505886E-5	0.0	0.0	0.0	1.134683960258829E-4
8	7.56455973505886E-5	0.0	0.0	0.0	1.134683960258829E-4
9	9.455699668823575E-5	0.0	0.0	1.891139933764715E-5	1.512911947011772E-4
10	1.512911947011772E-4	0.0	0.0	3.78227986752943E-5	1.512911947011772E-4
11	1.7020259403882434E-4	0.0	0.0	5.673419801294145E-5	1.512911947011772E-4
12	1.7020259403882434E-4	0.0	0.0	7.56455973505886E-5	1.512911947011772E-4
13	1.7020259403882434E-4	0.0	0.0	1.134683960258829E-4	1.512911947011772E-4
14	2.0802539271411866E-4	0.0	0.0	1.3237979536353004E-4	2.269367920517658E-4
15	2.0802539271411866E-4	0.0	0.0	2.647595907270601E-4	2.269367920517658E-4
16	2.269367920517658E-4	0.0	0.0	8.510129701941217E-4	3.2149378874000154E-4
17	2.269367920517658E-4	0.0	0.0	0.0015696461450247134	3.2149378874000154E-4
18	2.4584819138941295E-4	0.0	0.0	0.0017587601384011849	4.160507854282373E-4
19	3.025823894023544E-4	0.0	0.0	0.0025530389105823652	4.160507854282373E-4
20	3.404051880776487E-4	0.0	0.0	0.0035931658741529585	4.160507854282373E-4
21	3.5931658741529586E-4	0.0	0.0	0.005805799596657675	4.538735841035316E-4
22	3.5931658741529586E-4	0.0	0.0	0.009871750454251812	4.727849834411787E-4
23	3.9713938609059013E-4	0.0	0.0	0.012273498170133	7.186331748305917E-4
24	3.9713938609059013E-4	0.0	0.0	0.016528563021103608	7.186331748305917E-4
25	3.9713938609059013E-4	0.0	0.0	0.019724589509165977	7.186331748305917E-4
26	3.9713938609059013E-4	0.0	0.0	0.022901704597890697	7.186331748305917E-4
27	3.9713938609059013E-4	0.0	0.0	0.028688592795210726	7.186331748305917E-4
28	3.9713938609059013E-4	0.0	0.0	0.057887793372537924	7.186331748305917E-4
29	3.9713938609059013E-4	0.0	0.0	0.13226632696750418	7.375445741682388E-4
30	3.9713938609059013E-4	0.0	0.0	0.24026932858480704	7.375445741682388E-4
31	4.160507854282373E-4	0.0	0.0	0.5867639872491781	7.375445741682388E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3675	0.0	19.431293	37
TACTTAC	995	0.0	17.849247	31
TCGTTAC	1025	0.0	17.326828	23
GTACTTA	1120	0.0	15.857142	30
CTCGTTA	1230	0.0	15.642276	22
AGTGTAC	1360	0.0	14.963236	27
CGTTACG	945	0.0	14.878307	24
GAGTACT	7455	0.0	14.814889	11
AGTACTT	7710	0.0	14.3249035	12
GTTACGG	1875	0.0	13.813333	25
GTACTTT	7955	0.0	13.790698	13
TACTTTT	8020	0.0	13.771196	14
TCTTATA	5995	0.0	13.701417	37
TACGGAT	2305	0.0	13.563992	27
TAATACT	520	0.0	13.519231	4
GGGTAAG	4015	0.0	13.270236	1
ATAATAC	420	0.0	13.214287	3
CCGATTT	1080	0.0	13.189814	21
GTAAGAG	4210	0.0	13.1828985	3
TTACATA	660	0.0	13.174243	34
>>END_MODULE