FastQCFastQC Report
Thu 9 Feb 2017
SRR2727288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6233127
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT515290.8266958141555595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144480.23179376900230014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139920.22447801881784216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119880.1923272219545663No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT84040.13482799243461588No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT63030.10112099432596192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC42900.018.19813537
TAGATCG2250.016.4444455
TATACTG5850.015.8119655
GAGTACT136300.015.47322111
TATACCG2550.015.2352945
AGTACTT138350.015.20383112
TAGTACT3450.015.0144934
TAATACT4400.014.7159094
GTACTTT143950.014.61236513
TACTTTT145800.014.43964314
TCTTATA72900.014.31275737
TATACAC17300.013.79479937
AGTCGGT9600.013.68229211
ACTTTTT155050.013.66172115
TATAGCG1102.4590944E-413.4545453
CTAATCG5950.013.36974819
TAGTCCC13650.013.2820515
TCGGTCG6650.013.07518916
ATAATAC3550.013.0281693
CTACTCG13300.012.79699314
Produced by FastQC (version 0.11.5)