##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727288.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6233127
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1364756405573	33.0	31.0	34.0	30.0	34.0
2	32.271256786521434	33.0	31.0	34.0	30.0	34.0
3	32.34747807962199	34.0	31.0	34.0	30.0	34.0
4	35.92814345030993	37.0	35.0	37.0	35.0	37.0
5	35.809209566883524	37.0	35.0	37.0	35.0	37.0
6	35.82898086305637	37.0	35.0	37.0	35.0	37.0
7	35.76123220335475	37.0	35.0	37.0	35.0	37.0
8	35.72651977089509	37.0	35.0	37.0	35.0	37.0
9	37.46374781068956	39.0	37.0	39.0	35.0	39.0
10	37.30643768368589	39.0	37.0	39.0	34.0	39.0
11	37.41073172422124	39.0	37.0	39.0	35.0	39.0
12	37.30785061815683	39.0	37.0	39.0	34.0	39.0
13	37.36333480771369	39.0	37.0	39.0	34.0	39.0
14	38.489526204102695	40.0	38.0	41.0	34.0	41.0
15	38.453643251613514	40.0	38.0	41.0	34.0	41.0
16	38.36916911848579	40.0	38.0	41.0	34.0	41.0
17	38.329892845116106	40.0	38.0	41.0	34.0	41.0
18	38.292745679656456	40.0	38.0	41.0	34.0	41.0
19	38.29076417021505	40.0	38.0	41.0	34.0	41.0
20	38.17720495667744	40.0	38.0	41.0	33.0	41.0
21	38.0934830944404	40.0	38.0	41.0	33.0	41.0
22	38.00901954989847	40.0	37.0	41.0	33.0	41.0
23	37.88685759170317	40.0	37.0	41.0	33.0	41.0
24	37.76543843884458	40.0	37.0	41.0	32.0	41.0
25	37.677117119545294	40.0	37.0	41.0	32.0	41.0
26	37.290141850150015	39.0	37.0	41.0	31.0	41.0
27	37.06105009572242	39.0	36.0	41.0	31.0	41.0
28	36.88861353218056	39.0	36.0	40.0	30.0	41.0
29	36.82216293683732	39.0	36.0	40.0	30.0	41.0
30	36.722836226503965	39.0	36.0	40.0	30.0	41.0
31	36.50535164773636	39.0	35.0	40.0	30.0	41.0
32	36.38144032682151	39.0	35.0	40.0	30.0	41.0
33	36.190184637662604	39.0	35.0	40.0	29.0	41.0
34	36.03152125730793	39.0	35.0	40.0	28.0	41.0
35	35.85800834797686	38.0	35.0	40.0	27.0	41.0
36	35.68979069414116	38.0	35.0	40.0	27.0	41.0
37	35.43301829081936	38.0	34.0	40.0	26.0	41.0
38	35.298329233465	38.0	34.0	40.0	25.0	41.0
39	35.12467867251863	38.0	34.0	40.0	25.0	41.0
40	34.96500825348176	38.0	34.0	40.0	24.0	41.0
41	34.797169703104075	38.0	33.0	40.0	24.0	41.0
42	34.551718423192725	38.0	33.0	40.0	23.0	41.0
43	30.998662629527683	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	13.0
12	27.0
13	26.0
14	82.0
15	147.0
16	289.0
17	553.0
18	1039.0
19	2021.0
20	3735.0
21	6736.0
22	11492.0
23	18055.0
24	26827.0
25	38188.0
26	51229.0
27	67179.0
28	86427.0
29	108180.0
30	134323.0
31	166532.0
32	207737.0
33	259517.0
34	332320.0
35	434969.0
36	606475.0
37	949670.0
38	1612684.0
39	1106647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84694873054889	16.921137656909604	12.549511665653531	26.682401946887975
2	19.11121015182267	20.926639229394812	33.16792678859263	26.79422383018989
3	19.2784616774213	21.207573662465084	30.09649891619407	29.417465743919546
4	15.168020802399823	14.541481346361143	31.590901324487692	38.69959652675134
5	15.458452876060443	36.2775858730297	32.98812939316013	15.275831857749731
6	38.39641002020334	33.53488545957751	12.86729758594683	15.201406934272315
7	31.573045118445364	29.76210816817947	18.84449009301431	19.820356620360855
8	30.16413751877669	33.542313512944624	17.771208576369453	18.52234039190923
9	27.64976551897627	13.080449026628207	16.510926217290294	42.75885923710523
10	16.291132845520394	27.14887728101802	32.0692968392911	24.490693034170487
11	38.98439739796735	19.935162559659062	19.1810627314348	21.899377310938796
12	22.956631559087437	24.59547190358868	28.09268927137214	24.355207265951744
13	34.0426562783014	17.860425433333862	22.336429211212927	25.760489077151803
14	23.504831523567546	18.73781811280277	24.765081796023086	32.9922685676066
15	27.68119436680819	26.28618027516526	20.570301230826836	25.46232412719972
16	26.482486238448217	24.13456359865602	22.797305429521973	26.58564473337379
17	25.95016915265805	24.65409416493519	24.22323498301896	25.172501699387805
18	25.99961463965037	23.254459599491557	24.946226829647465	25.799698931210614
19	26.911019140152288	24.06658808652543	24.28070212591529	24.74169064740699
20	26.331951843753544	23.690019471767542	24.285290513092388	25.69273817138653
21	25.868316175813522	24.467462318672474	23.98364416447796	25.68057734103605
22	26.567820613955085	23.931615704284543	23.271417380072634	26.229146301687738
23	25.90757415980775	23.488387128964323	23.728186510558825	26.875852200669105
24	25.885177696523755	23.583363534867814	23.863046589617056	26.668412178991378
25	26.18574914324704	23.136332694649088	23.907406346766237	26.770511815337635
26	25.947489919586108	23.30672870936209	24.48265533495467	26.263126036097127
27	26.65735833715565	23.686233250180848	24.25615585884902	25.400252553814482
28	26.14700454523067	23.975798985003834	24.125579344043526	25.75161712572197
29	25.747750687576236	24.075476081267073	24.39350265123749	25.783270579919197
30	25.743980509301352	24.281664724623774	24.294467287446576	25.679887478628306
31	25.535096589560908	23.43536398343881	24.118424026977152	26.911115400023135
32	25.128944107829028	23.845623552993544	24.35175795391302	26.673674385264412
33	25.821469705334092	23.567737349166798	24.23762583371075	26.373167111788355
34	25.87909728134851	23.591353104148208	24.57244975114417	25.95709986335911
35	25.655228908379375	23.727705211204587	24.765049709399474	25.85201617101657
36	25.31223894523567	23.251764323107807	25.0077048004958	26.428291931160715
37	24.83856016410383	22.934748481781295	25.285205964839157	26.94148538927572
38	24.83068289800609	22.744394587179116	25.874316374429718	26.550606140385074
39	24.531362829603825	22.425982977725305	26.289773977010256	26.752880215660614
40	24.799655132969374	22.394602259828815	26.916056740060007	25.889685867141804
41	24.338409918488747	22.025381481879	27.265608417733183	26.370600181899068
42	23.66804013459055	22.211724548529173	27.687226652048004	26.433008664832276
43	23.803301296443983	21.264094249964746	27.594945522528256	27.337658931063014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	367.5
2	488.0
3	1727.5
4	2967.0
5	2967.0
6	3495.5
7	4024.0
8	3948.0
9	3872.0
10	5492.5
11	7113.0
12	7113.0
13	15978.5
14	24844.0
15	47650.0
16	70456.0
17	49152.0
18	27848.0
19	27848.0
20	30729.5
21	33611.0
22	24848.5
23	16086.0
24	20007.5
25	23929.0
26	23929.0
27	30257.0
28	36585.0
29	43775.0
30	50965.0
31	59850.5
32	68736.0
33	68736.0
34	82032.5
35	95329.0
36	115829.5
37	136330.0
38	165374.0
39	194418.0
40	194418.0
41	226359.0
42	258300.0
43	295894.5
44	333489.0
45	379168.0
46	424847.0
47	424847.0
48	478137.0
49	531427.0
50	563863.0
51	596299.0
52	620962.0
53	645625.0
54	645625.0
55	614175.0
56	582725.0
57	543286.5
58	503848.0
59	473236.0
60	442624.0
61	442624.0
62	394407.5
63	346191.0
64	297447.5
65	248704.0
66	211144.5
67	173585.0
68	173585.0
69	143968.5
70	114352.0
71	97037.0
72	79722.0
73	66412.0
74	53102.0
75	53102.0
76	44261.5
77	35421.0
78	30115.5
79	24810.0
80	20018.5
81	15227.0
82	15227.0
83	12495.5
84	9764.0
85	7992.0
86	6220.0
87	5447.0
88	4674.0
89	4674.0
90	3488.5
91	2303.0
92	1729.0
93	1155.0
94	864.0
95	573.0
96	573.0
97	382.0
98	191.0
99	146.0
100	101.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6233127.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.735622017976205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7457979545229	46.11888518369117
2	9.296938396013378	10.36341288729223
3	2.9559347833429275	4.942525913825693
4	1.3924447850470243	3.104351048811323
5	0.8059523628114326	2.2460128129076415
6	0.5181180679865506	1.7326579678789484
7	0.37115013494794347	1.4480398543365607
8	0.2688063021617561	1.1985669162670032
9	0.20198842503288886	1.0132155458675476
>10	1.2808678095813733	13.441572566434271
>50	0.10460782548090956	4.013797595150705
>100	0.051558018274795885	5.200299137089381
>500	0.003736808129043069	1.4029567270556609
>1k	0.001839628858896476	1.7956707711944342
>5k	1.4372100452067458E-4	0.5048141213841948
>10k+	1.1497680361653966E-4	1.4732209508132783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	51529	0.8266958141555595	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14448	0.23179376900230014	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13992	0.22447801881784216	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11988	0.1923272219545663	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	8404	0.13482799243461588	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	6303	0.10112099432596192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2086623616043764E-5
2	0.0	0.0	0.0	0.0	3.2086623616043764E-5
3	1.6043311808021882E-5	0.0	0.0	0.0	3.2086623616043764E-5
4	1.6043311808021882E-5	0.0	0.0	0.0	3.2086623616043764E-5
5	1.6043311808021882E-5	0.0	0.0	0.0	3.2086623616043764E-5
6	1.6043311808021882E-5	0.0	0.0	0.0	6.417324723208753E-5
7	4.812993542406564E-5	0.0	0.0	0.0	6.417324723208753E-5
8	6.417324723208753E-5	0.0	0.0	0.0	6.417324723208753E-5
9	1.4438980627219693E-4	0.0	0.0	0.0	1.4438980627219693E-4
10	1.9251974169626256E-4	0.0	0.0	1.6043311808021882E-5	1.4438980627219693E-4
11	3.208662361604376E-4	0.0	0.0	1.6043311808021882E-5	1.4438980627219693E-4
12	3.208662361604376E-4	0.0	0.0	8.02165590401094E-5	1.4438980627219693E-4
13	3.369095479684595E-4	0.0	0.0	1.4438980627219693E-4	1.604331180802188E-4
14	3.6899617158450324E-4	0.0	0.0	2.406496771203282E-4	2.406496771203282E-4
15	3.6899617158450324E-4	0.0	0.0	3.5295285977648137E-4	2.406496771203282E-4
16	4.01082795200547E-4	0.0	0.0	9.786420202893347E-4	3.0482292435241574E-4
17	4.01082795200547E-4	0.0	0.0	0.0018610241697305382	3.208662361604376E-4
18	4.01082795200547E-4	0.0	0.0	0.002454626706627348	3.6899617158450324E-4
19	4.4921273062461267E-4	0.0	0.0	0.0038503948339252514	3.850394833925251E-4
20	5.45472601472744E-4	1.6043311808021882E-5	0.0	0.005567029197383592	4.01082795200547E-4
21	5.936025368968096E-4	1.6043311808021882E-5	0.0	0.009273034225036647	4.01082795200547E-4
22	5.936025368968096E-4	1.6043311808021882E-5	0.0	0.015257189529428809	4.01082795200547E-4
23	5.936025368968096E-4	1.6043311808021882E-5	0.0	0.019011324492505928	4.973426660486782E-4
24	5.936025368968096E-4	1.6043311808021882E-5	0.0	0.02542864921571468	4.973426660486782E-4
25	6.096458487048315E-4	1.6043311808021882E-5	0.0	0.030177469510889156	4.973426660486782E-4
26	6.096458487048315E-4	1.6043311808021882E-5	0.0	0.03622579806251341	5.294292896647221E-4
27	6.096458487048315E-4	1.6043311808021882E-5	0.0	0.044407887084604566	5.294292896647221E-4
28	6.417324723208752E-4	1.6043311808021882E-5	0.0	0.07960691319140457	5.294292896647221E-4
29	6.577757841288971E-4	1.6043311808021882E-5	0.0	0.16882377015581426	5.294292896647221E-4
30	6.73819095936919E-4	3.2086623616043764E-5	0.0	0.29648040221224436	5.294292896647221E-4
31	6.73819095936919E-4	3.2086623616043764E-5	0.0	0.6594282452451233	5.45472601472744E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4290	0.0	18.198135	37
TAGATCG	225	0.0	16.444445	5
TATACTG	585	0.0	15.811965	5
GAGTACT	13630	0.0	15.473221	11
TATACCG	255	0.0	15.235294	5
AGTACTT	13835	0.0	15.203831	12
TAGTACT	345	0.0	15.014493	4
TAATACT	440	0.0	14.715909	4
GTACTTT	14395	0.0	14.612365	13
TACTTTT	14580	0.0	14.439643	14
TCTTATA	7290	0.0	14.312757	37
TATACAC	1730	0.0	13.794799	37
AGTCGGT	960	0.0	13.682292	11
ACTTTTT	15505	0.0	13.661721	15
TATAGCG	110	2.4590944E-4	13.454545	3
CTAATCG	595	0.0	13.369748	19
TAGTCCC	1365	0.0	13.282051	5
TCGGTCG	665	0.0	13.075189	16
ATAATAC	355	0.0	13.028169	3
CTACTCG	1330	0.0	12.796993	14
>>END_MODULE