FastQCFastQC Report
Thu 9 Feb 2017
SRR2727287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089576
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT19820.1819056220034215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.18144672790149563No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG15010.13776000939815122No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT14880.1365668847331439No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG13800.12665477213154475No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG13350.12252472521421176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG358.869097E-426.4285725
TACTTAC851.9772415E-923.94117731
CTACTAT502.7019772E-422.21
TCTATAC603.7260572E-521.5833343
TAATCGA603.7260572E-521.58333420
TAGGACG555.142859E-420.1818184
GTGTACG656.90171E-519.9230771
CTAGGTA656.90171E-519.9230774
TGATATG1103.8509825E-818.533
TAAACGT500.00703465318.54
GTCTAGT500.00703465318.51
ACTAATC701.2191232E-418.518
ATTCTAG609.236394E-418.51
GGTGATT2300.017.69565215
GTAAGTA953.6060392E-617.5263162
CTAATCG752.0671412E-417.26666819
GACTAAT752.0671412E-417.26666817
CGGTGAT2150.017.20930314
TATACAC2050.017.14634137
GTATAGA650.001579862817.0769231
Produced by FastQC (version 0.11.5)