##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1089576
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05568129253948	33.0	31.0	34.0	30.0	34.0
2	32.19742174937774	33.0	31.0	34.0	30.0	34.0
3	32.26930934602084	34.0	31.0	34.0	30.0	34.0
4	35.877444987775064	37.0	35.0	37.0	35.0	37.0
5	35.74083221363173	37.0	35.0	37.0	35.0	37.0
6	35.75417960748034	37.0	35.0	37.0	35.0	37.0
7	35.69056403591856	37.0	35.0	37.0	33.0	37.0
8	35.64867434671836	37.0	35.0	37.0	33.0	37.0
9	37.390864886891784	39.0	37.0	39.0	34.0	39.0
10	37.217980205144016	39.0	37.0	39.0	34.0	39.0
11	37.32170954573155	39.0	37.0	39.0	34.0	39.0
12	37.21051399810568	39.0	37.0	39.0	34.0	39.0
13	37.261063936797434	39.0	37.0	39.0	34.0	39.0
14	38.372248470964855	40.0	38.0	41.0	34.0	41.0
15	38.32997147514262	40.0	38.0	41.0	34.0	41.0
16	38.22056286114966	40.0	38.0	41.0	33.0	41.0
17	38.16033851700111	40.0	38.0	41.0	33.0	41.0
18	38.09160535841465	40.0	37.0	41.0	33.0	41.0
19	38.08265600563889	40.0	37.0	41.0	33.0	41.0
20	37.94317789672313	40.0	37.0	41.0	33.0	41.0
21	37.84443948838815	40.0	37.0	41.0	33.0	41.0
22	37.759985535657904	40.0	37.0	41.0	32.0	41.0
23	37.59938728459511	39.0	37.0	41.0	32.0	41.0
24	37.46844644155158	39.0	37.0	41.0	32.0	41.0
25	37.35813105281321	39.0	37.0	41.0	31.0	41.0
26	36.9277306034641	39.0	36.0	40.0	30.0	41.0
27	36.684319404979554	39.0	36.0	40.0	30.0	41.0
28	36.468165598361196	39.0	36.0	40.0	30.0	41.0
29	36.416239895151875	39.0	36.0	40.0	30.0	41.0
30	36.32304309199175	39.0	35.0	40.0	30.0	41.0
31	36.099588280211755	39.0	35.0	40.0	28.0	41.0
32	35.97420648031895	39.0	35.0	40.0	27.0	41.0
33	35.75884839607333	38.0	35.0	40.0	27.0	41.0
34	35.5846108945131	38.0	35.0	40.0	26.0	41.0
35	35.41290373503087	38.0	34.0	40.0	25.0	41.0
36	35.21462018252972	38.0	34.0	40.0	25.0	41.0
37	34.95566348744833	38.0	34.0	40.0	24.0	41.0
38	34.8222693965359	38.0	33.0	40.0	24.0	41.0
39	34.63349780097946	38.0	33.0	40.0	23.0	41.0
40	34.48743823285388	38.0	33.0	40.0	23.0	41.0
41	34.30353550371888	38.0	33.0	40.0	22.0	41.0
42	34.07366535239396	38.0	33.0	40.0	21.0	41.0
43	30.300072688825747	34.0	26.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	5.0
13	10.0
14	19.0
15	28.0
16	53.0
17	112.0
18	234.0
19	430.0
20	848.0
21	1481.0
22	2613.0
23	4068.0
24	5949.0
25	8126.0
26	10819.0
27	13807.0
28	17546.0
29	21703.0
30	26651.0
31	32521.0
32	39713.0
33	48477.0
34	61472.0
35	78891.0
36	108524.0
37	162823.0
38	274593.0
39	168051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67673663883932	15.294114407806338	11.379380603096985	28.64976835025735
2	20.71291952098798	18.988395485950498	31.410567046263864	28.888117946797653
3	20.153068716638398	19.686189857339002	28.640682247039216	31.520059178983384
4	16.69117161170951	13.653935108702836	29.499181332922163	40.15571194666549
5	16.6850224307437	34.75361057879395	32.27383863080684	16.287528359655497
6	40.91224476310051	31.09420545239616	12.153902068327497	15.839647716175834
7	33.09929734135113	28.47685705265167	17.90549718422579	20.518348421771403
8	31.289143666894276	32.26007180774907	17.12941547904873	19.32136904630792
9	28.730900827477846	12.20502287128204	15.23207192522596	43.832004376014154
10	17.284980579601605	25.50414106037578	31.146152264734173	26.064726095288442
11	40.74282106066947	18.71305902479497	17.718176611819644	22.825943302715917
12	24.149485671490563	22.954708987716323	26.2402071998649	26.655598140928216
13	35.49316431345771	16.747523807426006	20.685569432513198	27.073742446603084
14	24.68125215680228	17.790039428181238	22.44607076514167	35.08263764987481
15	29.808751294081365	24.43418357232538	19.018590717857222	26.73847441573603
16	28.258607017775724	22.980223499783403	21.254965234182837	27.506204248258037
17	27.75400706329802	23.0588779488535	21.86813953317621	27.318975454672277
18	27.5473211597906	22.41560019677379	22.806302635153493	27.23077600828212
19	29.087094429392717	22.70240901047747	21.907604425941834	26.302892134187978
20	28.19280160355955	21.855290498322283	22.07097072622745	27.880937171890718
21	27.67232391315521	23.109815194167272	21.93440384149431	27.283457051183213
22	28.865081462880976	22.167522045272655	20.614073731433145	28.353322760413224
23	27.89268486090002	22.14457734017636	21.431914799885458	28.530822999038158
24	27.583114899740817	21.863458813336564	21.796643832096155	28.756782454826464
25	28.31826325102609	21.303332672525826	21.46486339640374	28.91354068004435
26	27.870657944007576	21.684765450046623	22.3158366190151	28.128739986930697
27	29.220173718951227	21.794165803945756	21.830877332099828	27.154783145003194
28	28.058712746976806	22.725537273214535	21.290024743569976	27.925725236238684
29	27.92122807403981	22.470759267825283	22.028844247670655	27.579168410464256
30	28.26154394002805	22.37411616995969	21.786181046572246	27.578158843440015
31	27.79631709949558	21.804812147110436	21.75433379589859	28.644536957495394
32	27.158362518998214	22.03389208279184	21.870892897787762	28.936852500422184
33	27.79328839842287	22.00764334016168	21.820965219498227	28.378103041917225
34	28.162698150473215	21.83610872486178	22.37549285226547	27.625700272399538
35	27.802558059281772	22.048576694053466	22.494162867023505	27.654702379641254
36	27.289973347430564	21.38281313097939	23.00234219549623	28.32487132609382
37	26.735904608765242	21.27469768056565	23.403232082938686	28.58616562773042
38	27.245001725441824	20.860591642987732	23.402497852375603	28.49190877919484
39	26.150998186450508	20.898312738166037	24.37544512727887	28.575243948104585
40	27.187915299162242	20.322308861428663	24.738246804261475	27.751529035147616
41	26.250119312466502	20.412710999508064	25.14271606569895	28.19445362232648
42	25.43943699200423	20.2967025705412	26.046553888852173	28.217306548602394
43	25.77433790758974	19.16286702350272	25.4978083217692	29.564986747138338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	13.5
2	18.0
3	74.0
4	130.0
5	130.0
6	166.5
7	203.0
8	174.5
9	146.0
10	208.5
11	271.0
12	271.0
13	612.0
14	953.0
15	1839.5
16	2726.0
17	1884.0
18	1042.0
19	1042.0
20	1221.0
21	1400.0
22	1128.5
23	857.0
24	1093.0
25	1329.0
26	1329.0
27	1653.5
28	1978.0
29	2487.0
30	2996.0
31	3733.0
32	4470.0
33	4470.0
34	5708.5
35	6947.0
36	8815.0
37	10683.0
38	14117.5
39	17552.0
40	17552.0
41	22002.5
42	26453.0
43	32978.0
44	39503.0
45	49011.5
46	58520.0
47	58520.0
48	72425.0
49	86330.0
50	98354.0
51	110378.0
52	118347.5
53	126317.0
54	126317.0
55	122790.0
56	119263.0
57	113544.0
58	107825.0
59	103547.0
60	99269.0
61	99269.0
62	89263.5
63	79258.0
64	68176.0
65	57094.0
66	49211.5
67	41329.0
68	41329.0
69	34508.0
70	27687.0
71	23506.0
72	19325.0
73	16188.0
74	13051.0
75	13051.0
76	10861.5
77	8672.0
78	7359.5
79	6047.0
80	4869.5
81	3692.0
82	3692.0
83	3045.5
84	2399.0
85	1954.5
86	1510.0
87	1266.5
88	1023.0
89	1023.0
90	768.5
91	514.0
92	374.5
93	235.0
94	179.5
95	124.0
96	124.0
97	77.5
98	31.0
99	24.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1089576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.0408655590639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.36401891020951	57.89918580899808
2	7.476731985327813	10.024931676990297
3	2.3545286695020544	4.7354891996114645
4	1.1540561588229934	3.0947569516504796
5	0.6670376727719467	2.235939147156747
6	0.41324937337805406	1.662275740980331
7	0.30305099300825444	1.4221760615865042
8	0.2197041022376444	1.1783322544711
9	0.1587165377985862	0.9576444665299592
>10	0.8129574257016505	9.470853985976024
>50	0.04744826806542013	2.1963831830232436
>100	0.025622511133489567	3.2974461083481206
>500	0.0019182613616948673	0.843444760487915
>1k	9.591306808474336E-4	0.9811406541898736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	1982	0.1819056220034215	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1977	0.18144672790149563	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1501	0.13776000939815122	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	1488	0.1365668847331439	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	1380	0.12665477213154475	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	1335	0.12252472521421176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.177882038517735E-5
2	0.0	0.0	0.0	0.0	9.177882038517735E-5
3	0.0	0.0	0.0	0.0	9.177882038517735E-5
4	0.0	0.0	0.0	0.0	9.177882038517735E-5
5	0.0	0.0	0.0	0.0	9.177882038517735E-5
6	0.0	0.0	0.0	0.0	9.177882038517735E-5
7	0.0	0.0	0.0	0.0	9.177882038517735E-5
8	0.0	0.0	0.0	0.0	9.177882038517735E-5
9	9.177882038517735E-5	0.0	0.0	0.0	1.835576407703547E-4
10	9.177882038517735E-5	0.0	0.0	0.0	1.835576407703547E-4
11	2.7533646115553205E-4	0.0	0.0	0.0	1.835576407703547E-4
12	2.7533646115553205E-4	0.0	0.0	0.0	2.7533646115553205E-4
13	3.671152815407094E-4	0.0	0.0	0.0	2.7533646115553205E-4
14	3.671152815407094E-4	0.0	0.0	0.0	4.588941019258868E-4
15	3.671152815407094E-4	0.0	0.0	0.0	4.588941019258868E-4
16	3.671152815407094E-4	0.0	0.0	4.588941019258868E-4	5.506729223110641E-4
17	3.671152815407094E-4	0.0	0.0	8.260093834665962E-4	5.506729223110641E-4
18	3.671152815407094E-4	0.0	0.0	9.177882038517736E-4	5.506729223110641E-4
19	4.588941019258868E-4	0.0	0.0	0.0013766823057776603	5.506729223110641E-4
20	4.588941019258868E-4	0.0	0.0	0.0022026916892442564	6.424517426962415E-4
21	5.506729223110641E-4	0.0	0.0	0.0032122587134812072	6.424517426962415E-4
22	6.424517426962415E-4	0.0	0.0	0.0072505268104290106	6.424517426962415E-4
23	6.424517426962415E-4	0.0	0.0	0.009820333781213976	0.0010095670242369508
24	6.424517426962415E-4	0.0	0.0	0.013124371315080362	0.0010095670242369508
25	6.424517426962415E-4	0.0	0.0	0.014868168902398731	0.0011013458446221282
26	6.424517426962415E-4	0.0	0.0	0.01762153351395405	0.0011013458446221282
27	6.424517426962415E-4	0.0	0.0	0.022761147455523982	0.0011013458446221282
28	6.424517426962415E-4	0.0	0.0	0.051763254697240024	0.0011013458446221282
29	6.424517426962415E-4	0.0	0.0	0.1365668847331439	0.0011931246650073056
30	6.424517426962415E-4	0.0	0.0	0.2491794973457565	0.0011931246650073056
31	6.424517426962415E-4	0.0	0.0	0.5357129745882803	0.0011931246650073056
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACCG	35	8.869097E-4	26.428572	5
TACTTAC	85	1.9772415E-9	23.941177	31
CTACTAT	50	2.7019772E-4	22.2	1
TCTATAC	60	3.7260572E-5	21.583334	3
TAATCGA	60	3.7260572E-5	21.583334	20
TAGGACG	55	5.142859E-4	20.181818	4
GTGTACG	65	6.90171E-5	19.923077	1
CTAGGTA	65	6.90171E-5	19.923077	4
TGATATG	110	3.8509825E-8	18.5	33
TAAACGT	50	0.007034653	18.5	4
GTCTAGT	50	0.007034653	18.5	1
ACTAATC	70	1.2191232E-4	18.5	18
ATTCTAG	60	9.236394E-4	18.5	1
GGTGATT	230	0.0	17.695652	15
GTAAGTA	95	3.6060392E-6	17.526316	2
CTAATCG	75	2.0671412E-4	17.266668	19
GACTAAT	75	2.0671412E-4	17.266668	17
CGGTGAT	215	0.0	17.209303	14
TATACAC	205	0.0	17.146341	37
GTATAGA	65	0.0015798628	17.076923	1
>>END_MODULE