FastQCFastQC Report
Thu 9 Feb 2017
SRR2727286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039454
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC17380.1672031662776804No Hit
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG13660.13141514679822291No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT13280.12775938136752563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.10265004511984177No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA10570.10168800158544775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACTA250.005496104729.69
CGTTATG400.001931063123.12500224
GGTATCA10800.021.0694451
TACTTAC1500.020.96666731
GTCGGTA450.00382556620.55555525
TAGAGTA701.2190548E-418.55
TCGTTAT500.007034440518.523
CTTATAC6400.018.537
AGGTTAT500.007034440518.51
TAAGGGT1002.874258E-718.54
GTAAGGT609.236007E-418.53
CGGTACG500.007034440518.527
ACTTACA1451.546141E-1017.86206832
TAGTACT650.00157979617.0769254
CTTGTTA4250.016.9764722
TGTAGTC4700.016.9255333
CTCGTTA4750.016.74736822
TAGTCCC5000.016.655
TCGTTAC4350.016.58620823
TTATACA2600.016.36538737
Produced by FastQC (version 0.11.5)