FastQCFastQC Report
Thu 9 Feb 2017
SRR2727285.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727285.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8048493
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT182150.2263156593414444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC43100.019.35846937
TACTTAC11450.016.48034931
TCTTATA75750.015.53267337
GTATAGA5800.014.9913791
GTCTTAG11850.014.8312241
TCGTTAC15450.014.24919223
GGGTAAG56500.014.0469031
CTCGTTA18350.014.01362422
GAGTACT53650.013.62068911
GTATTAA6100.013.3442621
GTAAGAG60250.013.3261413
TATACCG2800.013.2142865
TTAGGCA24000.013.1812514
CTCTTAT117600.013.07270337
TAATACC5850.012.9658124
TAATACT6850.012.9635044
TTAATAC6000.012.953
AGTACTT55750.012.9085212
GTTACGG24550.012.88594725
CTATACT5050.012.8217814
Produced by FastQC (version 0.11.5)