##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727285.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8048493
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03510371444692	33.0	31.0	34.0	30.0	34.0
2	32.17980409500263	33.0	31.0	34.0	30.0	34.0
3	32.25925462070974	34.0	31.0	34.0	30.0	34.0
4	35.86944139729015	37.0	35.0	37.0	35.0	37.0
5	35.7340628860583	37.0	35.0	37.0	35.0	37.0
6	35.74566642475803	37.0	35.0	37.0	35.0	37.0
7	35.68608185408125	37.0	35.0	37.0	33.0	37.0
8	35.64444561236495	37.0	35.0	37.0	33.0	37.0
9	37.37119358866312	39.0	37.0	39.0	34.0	39.0
10	37.18743247959587	39.0	37.0	39.0	34.0	39.0
11	37.3045139009253	39.0	37.0	39.0	34.0	39.0
12	37.193827341342036	39.0	37.0	39.0	34.0	39.0
13	37.253904675073954	39.0	37.0	39.0	34.0	39.0
14	38.380784949430904	40.0	38.0	41.0	34.0	41.0
15	38.338607239889505	40.0	38.0	41.0	34.0	41.0
16	38.252839258231326	40.0	38.0	41.0	33.0	41.0
17	38.19875161722822	40.0	38.0	41.0	33.0	41.0
18	38.13089357225011	40.0	38.0	41.0	33.0	41.0
19	38.12906279473685	40.0	38.0	41.0	33.0	41.0
20	37.99425122193683	40.0	37.0	41.0	33.0	41.0
21	37.90486355644467	40.0	37.0	41.0	33.0	41.0
22	37.829637299802584	40.0	37.0	41.0	33.0	41.0
23	37.69792630744662	40.0	37.0	41.0	32.0	41.0
24	37.57151270430377	39.0	37.0	41.0	32.0	41.0
25	37.47387467442663	39.0	37.0	41.0	32.0	41.0
26	37.079521719159104	39.0	36.0	40.0	31.0	41.0
27	36.84863886941319	39.0	36.0	40.0	30.0	41.0
28	36.655252977172246	39.0	36.0	40.0	30.0	41.0
29	36.61851529224167	39.0	36.0	40.0	30.0	41.0
30	36.539242812287966	39.0	36.0	40.0	30.0	41.0
31	36.32307650637206	39.0	35.0	40.0	30.0	41.0
32	36.20465533112845	39.0	35.0	40.0	29.0	41.0
33	36.016161907576986	38.0	35.0	40.0	28.0	41.0
34	35.86395353763742	38.0	35.0	40.0	27.0	41.0
35	35.69399998235694	38.0	35.0	40.0	27.0	41.0
36	35.50727359767847	38.0	34.0	40.0	26.0	41.0
37	35.26241185772293	38.0	34.0	40.0	25.0	41.0
38	35.13185375200053	38.0	34.0	40.0	25.0	41.0
39	34.95006767105345	38.0	34.0	40.0	24.0	41.0
40	34.81887354564389	38.0	33.0	40.0	24.0	41.0
41	34.661261679670964	38.0	33.0	40.0	23.0	41.0
42	34.44935542591638	38.0	33.0	40.0	23.0	41.0
43	30.840073539232748	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	27.0
11	29.0
12	35.0
13	42.0
14	86.0
15	155.0
16	348.0
17	711.0
18	1307.0
19	2730.0
20	5211.0
21	9564.0
22	16204.0
23	25946.0
24	37885.0
25	53847.0
26	71887.0
27	93271.0
28	119199.0
29	149605.0
30	186243.0
31	230217.0
32	282917.0
33	350815.0
34	445138.0
35	580154.0
36	798742.0
37	1220857.0
38	2109525.0
39	1255786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56175746192486	17.073109214358514	11.885343007691004	27.47979031602562
2	19.614678176398986	20.279374039338794	33.10158808611749	27.004359698144732
3	19.0561885311946	20.962856027830306	29.874350390812292	30.106605050162806
4	15.574965400355072	14.612213739888947	31.82800805069968	37.98481280905631
5	15.27206397520629	36.405237601623064	33.31083222660441	15.01186619656624
6	38.676532364506	33.63101639027331	12.966974065828223	14.725477179392465
7	32.00534559699561	30.16280190589717	18.705340241955852	19.12651225515137
8	29.872399715077098	34.05718312732582	17.628542386754887	18.441874770842194
9	28.537715072871407	12.67731735618084	16.015432951236956	42.7695346197108
10	16.1391455518443	26.83779435479412	32.35715058707264	24.66590950628894
11	39.703432679881814	19.74944874773451	19.208763677871126	21.338354894512552
12	23.080954409726143	23.93569827295619	27.77483933948877	25.20850797782889
13	33.68023057235684	18.094405996252963	21.847593083574775	26.37777034781542
14	23.647805868750833	18.68189485907486	23.635058140697893	34.03524113147641
15	27.926370812523537	26.28584009453695	19.864203149583407	25.923585943356102
16	26.868185137267314	24.72452917583453	21.971678424768463	26.435607262129697
17	26.461127567608	24.38452763765838	22.846513005602414	26.307831789131207
18	26.32351174313005	24.14104106197272	23.57082251298473	25.9646246819125
19	27.898539515409905	23.78539684385636	23.123123794727785	25.192939846005952
20	26.904800687532436	23.478668615354454	23.08662006663856	26.52991063047455
21	26.65854340682163	24.12942398036502	23.27185971336497	25.94017289944838
22	27.643597379037292	23.312314491669433	22.172523477376448	26.871564651916824
23	26.622263323084212	23.599784456543603	22.879376300631684	26.8985759197405
24	26.364401385451906	23.249905292829354	22.957552426274084	27.428140895444653
25	26.94253445955659	22.759639599612	22.85327203490144	27.44455390592997
26	26.549392538454093	23.19109925299059	23.40161071147108	26.857897497084238
27	27.494302349520588	23.43683469688052	22.810195647806367	26.258667305792528
28	26.533314994496486	24.200654706415225	22.581270804360518	26.68475949472777
29	26.620759936052625	24.11953393014071	22.95715483631532	26.302551297491345
30	27.26796184080672	23.82595101965051	22.517668835644137	26.388418303898632
31	26.64999522270815	23.38450191855792	22.913308118675136	27.052194740058795
32	26.013254903744095	23.706326140806734	22.873027285977635	27.407391669471536
33	26.340906303826074	23.37766834114163	23.060751869946337	27.220673485085968
34	26.753629530397806	23.242587152650813	23.678109678420544	26.325673638530837
35	26.593562297935776	23.28120307739598	23.414656631993093	26.710577992675148
36	26.089865518923855	22.787967884174094	24.11489952218384	27.00726707471821
37	25.810943738163157	22.751402032653814	24.303965972263377	27.133688256919648
38	26.044304194586488	22.37971754463848	24.41838490758456	27.157593353190467
39	25.138258801989394	22.454700525924544	25.216049762359237	27.190990909726825
40	25.934233899439313	21.989669370402634	25.455386492850273	26.620710237307783
41	25.070320617785217	22.07536243120296	25.90496133872515	26.949355612286674
42	24.495678880505952	22.093229129975015	26.342061799643734	27.0690301898753
43	24.58153346222703	21.076927071937565	25.9808016233598	28.360737842475604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	186.5
2	258.0
3	731.0
4	1204.0
5	1204.0
6	1433.0
7	1662.0
8	1688.5
9	1715.0
10	2413.5
11	3112.0
12	3112.0
13	6637.0
14	10162.0
15	18630.0
16	27098.0
17	19946.5
18	12795.0
19	12795.0
20	15685.5
21	18576.0
22	16097.0
23	13618.0
24	18062.5
25	22507.0
26	22507.0
27	29387.0
28	36267.0
29	44497.5
30	52728.0
31	65488.0
32	78248.0
33	78248.0
34	96925.0
35	115602.0
36	142317.0
37	169032.0
38	207385.0
39	245738.0
40	245738.0
41	288607.5
42	331477.0
43	382975.0
44	434473.0
45	500154.0
46	565835.0
47	565835.0
48	634129.5
49	702424.0
50	752405.0
51	802386.0
52	818517.0
53	834648.0
54	834648.0
55	802650.5
56	770653.0
57	729105.5
58	687558.0
59	650402.0
60	613246.0
61	613246.0
62	542089.5
63	470933.0
64	401948.0
65	332963.0
66	283282.5
67	233602.0
68	233602.0
69	194023.5
70	154445.0
71	130703.0
72	106961.0
73	88167.0
74	69373.0
75	69373.0
76	57894.5
77	46416.0
78	39228.0
79	32040.0
80	25654.0
81	19268.0
82	19268.0
83	15688.5
84	12109.0
85	9788.5
86	7468.0
87	6315.0
88	5162.0
89	5162.0
90	3889.0
91	2616.0
92	1900.5
93	1185.0
94	875.0
95	565.0
96	565.0
97	373.5
98	182.0
99	125.0
100	68.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8048493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.794510057771916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31591034534593	42.93033646584245
2	9.403684012479346	9.929257803538995
3	3.3141583145904367	5.249080934180796
4	1.6024780868928823	3.384081819033015
5	0.9570861035910295	2.526444596109517
6	0.6531061989412488	2.068825307327816
7	0.43892848766969417	1.62211101198442
8	0.31190196248088303	1.317336903618862
9	0.2595345299055148	1.2331798514492225
>10	1.5409220617122117	15.341828295573507
>50	0.13289304805495195	4.804405155856548
>100	0.06364609631262605	6.00724913770197
>500	0.0038654937269831497	1.4027560005993713
>1k	0.0018145611172400765	1.8216091150354476
>5k	4.7131452690603895E-5	0.13487732326840823
>10k+	2.3565726345301947E-5	0.2266202788796288
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18215	0.2263156593414444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7274058634330677E-5
2	0.0	0.0	0.0	0.0	3.7274058634330677E-5
3	1.2424686211443559E-5	0.0	0.0	0.0	3.7274058634330677E-5
4	1.2424686211443559E-5	0.0	0.0	0.0	3.7274058634330677E-5
5	1.2424686211443559E-5	0.0	0.0	0.0	3.7274058634330677E-5
6	1.2424686211443559E-5	0.0	0.0	0.0	9.939748969154847E-5
7	2.4849372422887118E-5	0.0	0.0	0.0	9.939748969154847E-5
8	2.4849372422887118E-5	0.0	0.0	0.0	1.242468621144356E-4
9	3.7274058634330677E-5	0.0	0.0	0.0	1.8637029317165337E-4
10	9.939748969154847E-5	0.0	0.0	0.0	1.9879497938309694E-4
11	1.1182217590299203E-4	0.0	0.0	2.4849372422887118E-5	1.9879497938309694E-4
12	1.242468621144356E-4	0.0	0.0	2.4849372422887118E-5	1.9879497938309694E-4
13	1.242468621144356E-4	0.0	0.0	2.4849372422887118E-5	2.360690380174276E-4
14	1.3667154832587913E-4	0.0	0.0	3.7274058634330677E-5	3.975899587661939E-4
15	1.3667154832587913E-4	0.0	0.0	4.9698744845774235E-5	3.975899587661939E-4
16	1.3667154832587913E-4	0.0	0.0	3.230418414975325E-4	4.22439331189081E-4
17	1.3667154832587913E-4	0.0	0.0	6.088096243607343E-4	4.22439331189081E-4
18	1.3667154832587913E-4	0.0	0.0	7.082071140522828E-4	4.721380760348552E-4
19	1.490962345373227E-4	0.0	0.0	9.81550210704041E-4	4.721380760348552E-4
20	1.6152092074876625E-4	0.0	0.0	0.0016276338936991061	4.721380760348552E-4
21	1.8637029317165337E-4	0.0	0.0	0.0028079790837862444	4.969874484577424E-4
22	1.8637029317165337E-4	0.0	0.0	0.004547435153388343	5.094121346691858E-4
23	1.8637029317165337E-4	0.0	0.0	0.005491711305458053	6.336589967836215E-4
24	1.8637029317165337E-4	0.0	0.0	0.007256016747483038	6.336589967836215E-4
25	1.8637029317165337E-4	1.2424686211443559E-5	0.0	0.008175443527129862	6.46083682995065E-4
26	1.9879497938309694E-4	1.2424686211443559E-5	0.0	0.00946761089311999	6.585083692065086E-4
27	1.9879497938309694E-4	1.2424686211443559E-5	0.0	0.013120468639284398	6.585083692065086E-4
28	1.9879497938309694E-4	2.4849372422887118E-5	0.0	0.03599431595455199	6.833577416293957E-4
29	1.9879497938309694E-4	2.4849372422887118E-5	0.0	0.09453943738287404	6.957824278408393E-4
30	1.9879497938309694E-4	2.4849372422887118E-5	0.0	0.17585900863677212	6.957824278408393E-4
31	1.9879497938309694E-4	2.4849372422887118E-5	0.0	0.46308047978671285	6.957824278408393E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4310	0.0	19.358469	37
TACTTAC	1145	0.0	16.480349	31
TCTTATA	7575	0.0	15.532673	37
GTATAGA	580	0.0	14.991379	1
GTCTTAG	1185	0.0	14.831224	1
TCGTTAC	1545	0.0	14.249192	23
GGGTAAG	5650	0.0	14.046903	1
CTCGTTA	1835	0.0	14.013624	22
GAGTACT	5365	0.0	13.620689	11
GTATTAA	610	0.0	13.344262	1
GTAAGAG	6025	0.0	13.326141	3
TATACCG	280	0.0	13.214286	5
TTAGGCA	2400	0.0	13.181251	4
CTCTTAT	11760	0.0	13.072703	37
TAATACC	585	0.0	12.965812	4
TAATACT	685	0.0	12.963504	4
TTAATAC	600	0.0	12.95	3
AGTACTT	5575	0.0	12.90852	12
GTTACGG	2455	0.0	12.885947	25
CTATACT	505	0.0	12.821781	4
>>END_MODULE