##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727284.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7543601
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.995082719777994	33.0	31.0	34.0	30.0	34.0
2	32.143461458261115	33.0	31.0	34.0	30.0	34.0
3	32.22061864088517	34.0	31.0	34.0	30.0	34.0
4	35.83018998486267	37.0	35.0	37.0	35.0	37.0
5	35.68412234422261	37.0	35.0	37.0	35.0	37.0
6	35.700457381030624	37.0	35.0	37.0	33.0	37.0
7	35.639447924141265	37.0	35.0	37.0	33.0	37.0
8	35.60565106770626	37.0	35.0	37.0	33.0	37.0
9	37.31274493441528	39.0	37.0	39.0	34.0	39.0
10	37.128461725374926	39.0	37.0	39.0	33.0	39.0
11	37.24471906719351	39.0	37.0	39.0	34.0	39.0
12	37.14220993395595	39.0	37.0	39.0	33.0	39.0
13	37.200331380198925	39.0	37.0	39.0	34.0	39.0
14	38.31209060500416	40.0	38.0	41.0	34.0	41.0
15	38.26758798616205	40.0	38.0	41.0	33.0	41.0
16	38.1783919907747	40.0	38.0	41.0	33.0	41.0
17	38.134934761263224	40.0	38.0	41.0	33.0	41.0
18	38.08428454792347	40.0	37.0	41.0	33.0	41.0
19	38.089364747684826	40.0	38.0	41.0	33.0	41.0
20	37.96478459027724	40.0	37.0	41.0	33.0	41.0
21	37.87453790305187	40.0	37.0	41.0	33.0	41.0
22	37.798546078988004	40.0	37.0	41.0	33.0	41.0
23	37.667292848601086	40.0	37.0	41.0	32.0	41.0
24	37.545204869663706	39.0	37.0	41.0	32.0	41.0
25	37.4417639533162	39.0	37.0	41.0	32.0	41.0
26	37.047398318124195	39.0	36.0	40.0	31.0	41.0
27	36.802535818105966	39.0	36.0	40.0	30.0	41.0
28	36.61568725599352	39.0	36.0	40.0	30.0	41.0
29	36.54954762321072	39.0	36.0	40.0	30.0	41.0
30	36.454581306726055	39.0	36.0	40.0	30.0	41.0
31	36.22442570862377	39.0	35.0	40.0	29.0	41.0
32	36.09592991463891	38.0	35.0	40.0	29.0	41.0
33	35.89479321613113	38.0	35.0	40.0	27.0	41.0
34	35.72337866225958	38.0	35.0	40.0	27.0	41.0
35	35.54596816560155	38.0	35.0	40.0	26.0	41.0
36	35.365484600789465	38.0	34.0	40.0	25.0	41.0
37	35.11466592679014	38.0	34.0	40.0	25.0	41.0
38	34.99147383325285	38.0	34.0	40.0	24.0	41.0
39	34.804465798230844	38.0	33.0	40.0	24.0	41.0
40	34.652717581430935	38.0	33.0	40.0	24.0	41.0
41	34.488495083448875	38.0	33.0	40.0	23.0	41.0
42	34.27030989576463	38.0	33.0	40.0	22.0	41.0
43	30.785641234206317	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	34.0
11	50.0
12	46.0
13	52.0
14	72.0
15	148.0
16	274.0
17	605.0
18	1158.0
19	2431.0
20	4864.0
21	9010.0
22	15868.0
23	25100.0
24	37284.0
25	51792.0
26	69774.0
27	91138.0
28	115489.0
29	144775.0
30	178896.0
31	220138.0
32	271628.0
33	335813.0
34	425084.0
35	551952.0
36	765437.0
37	1181133.0
38	1960057.0
39	1083484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.948188802668646	17.790508803421602	12.485058528413685	26.77624386549607
2	18.88901600177422	21.008812634708544	33.87928391228539	26.22288745123185
3	18.90463188601836	21.71798322843427	30.46236141068437	28.915023474863
4	15.073384713746126	15.054229405823557	32.6230006067394	37.249385273690905
5	14.87577351983489	36.94191938306387	33.573687155511	14.60861994159023
6	37.473204110344646	34.57366051041141	13.359680608770267	14.593454770473677
7	30.90375538154788	30.69328560723188	19.356113877178814	19.04684513404142
8	29.257817320932006	34.654828642182956	18.09082956534949	17.996524471535544
9	27.76115809942758	13.22556694077537	16.938687504813682	42.07458745498337
10	15.837383233816315	27.438420987536322	32.84514915356738	23.879046625079987
11	38.51901764157463	20.46822200696988	19.957444726994442	21.05531562446105
12	22.384680738018886	24.481318669956163	28.895390941275924	24.238609650749027
13	33.17798754202403	18.505194004826077	22.84811988332893	25.468698569820962
14	23.12913951838121	19.23447435780339	24.93584960286208	32.70053652095332
15	27.12500303237141	26.987257146818873	20.77918755246997	25.10855226833975
16	25.797613102813894	25.159390057878195	23.210135318662797	25.83286152064511
17	25.405757807179885	25.389677953539696	24.261556251450735	24.943007987829684
18	25.251852530376407	24.617725672394393	25.000420886523557	25.13000091070564
19	26.40220499467032	24.850704590552976	24.643933315136895	24.103157099639812
20	25.675072157183287	24.399673312520108	24.480350962358692	25.44490356793791
21	25.33807130043066	25.10908251907809	24.5615323503987	24.99131383009255
22	26.256134172525826	24.332477287703842	23.606948458700295	25.804440081070034
23	25.58443904973235	24.25077890519395	24.169239597905563	25.995542447168134
24	25.284502719589756	24.141096539968114	24.351036063545777	26.223364676896356
25	25.835950231195948	23.65821044882941	24.202260962635748	26.303578357338893
26	25.359997168461057	24.020437984458614	24.85937419012485	25.76019065695548
27	26.277026051616463	24.448244280152146	24.26141043249769	25.013319235733704
28	25.67293789796146	24.871821826207405	24.016951585854024	25.438288689977107
29	25.412730604389072	24.893760950506262	24.553565333055126	25.13994311204954
30	25.839211273236746	24.670512133396237	24.164096165743654	25.326180427623356
31	25.479674760104626	24.104032013358076	24.454408444985358	25.96188478155194
32	24.973603455431963	24.55579238615616	24.383951378128295	26.086652780283583
33	25.4304277227812	24.118706702541665	24.34802689060569	26.10283868407144
34	25.642103817526934	24.11345722023209	24.954421634972476	25.290017327268504
35	25.432707800956067	24.115366122890116	24.846847016431543	25.605079059722275
36	25.12078780412697	23.692000677130192	25.268422865949564	25.918788652793275
37	24.58885351969172	23.49994385970308	25.604230658540928	26.306971962064274
38	24.72211878650528	23.254583586804234	25.857465154904137	26.165832471786356
39	24.205985974072593	23.146770355431045	26.529027715013033	26.118215955483333
40	24.769576227586796	22.78887761958778	26.91528886535754	25.526257287467878
41	24.14687627301603	22.739020793915266	27.208954980519252	25.905147952549452
42	23.59530945499371	22.801325255670335	27.555434069219725	26.047931220116226
43	23.665925597072274	21.803525928797136	27.345242676541346	27.185305797589244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	256.0
1	392.0
2	528.0
3	1598.0
4	2668.0
5	2668.0
6	3156.0
7	3644.0
8	3745.5
9	3847.0
10	5502.0
11	7157.0
12	7157.0
13	15358.5
14	23560.0
15	43260.0
16	62960.0
17	44972.0
18	26984.0
19	26984.0
20	31741.0
21	36498.0
22	30604.0
23	24710.0
24	31439.5
25	38169.0
26	38169.0
27	48022.0
28	57875.0
29	69726.0
30	81577.0
31	97314.0
32	113051.0
33	113051.0
34	133508.5
35	153966.0
36	182572.0
37	211178.0
38	247745.5
39	284313.0
40	284313.0
41	323896.0
42	363479.0
43	407935.0
44	452391.0
45	503585.0
46	554779.0
47	554779.0
48	610278.5
49	665778.0
50	700165.5
51	734553.0
52	741750.5
53	748948.0
54	748948.0
55	703119.5
56	657291.0
57	609831.0
58	562371.0
59	522761.5
60	483152.0
61	483152.0
62	427611.0
63	372070.0
64	317943.5
65	263817.0
66	223935.5
67	184054.0
68	184054.0
69	153723.5
70	123393.0
71	104318.5
72	85244.0
73	70960.0
74	56676.0
75	56676.0
76	47247.5
77	37819.0
78	31794.5
79	25770.0
80	20900.0
81	16030.0
82	16030.0
83	12830.5
84	9631.0
85	7745.5
86	5860.0
87	4937.5
88	4015.0
89	4015.0
90	3004.5
91	1994.0
92	1466.5
93	939.0
94	667.5
95	396.0
96	396.0
97	270.0
98	144.0
99	105.0
100	66.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7543601.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.650379230517245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56067770740984	43.22110910070014
2	10.025869121617474	10.757833609806205
3	3.399794158082393	5.472007376604524
4	1.6402200481204579	3.5199371041263925
5	0.9674315415111076	2.59515345408174
6	0.6423566966999369	2.0677608227528377
7	0.43437888572570293	1.6313214364239432
8	0.34476564829607004	1.479744622139143
9	0.2583023548547173	1.2472217364682097
>10	1.5485281551687966	15.601346852098995
>50	0.12535684930255292	4.609257224004083
>100	0.04827120023555013	4.516711804615843
>500	0.0028137317910901607	0.9813090130916543
>1k	0.0011105110690220329	1.1572161260920137
>5k	4.935604615511967E-5	0.22317096162364244
>10k+	7.40340692326795E-5	0.9188987553706266
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44332	0.5876768933033442	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12645	0.16762551465805256	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12427	0.16473564813409405	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9976	0.13224453414224852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.060501476682025E-4
2	2.6512536917050624E-5	0.0	0.0	0.0	1.060501476682025E-4
3	2.6512536917050624E-5	0.0	0.0	0.0	1.060501476682025E-4
4	2.6512536917050624E-5	0.0	0.0	0.0	1.060501476682025E-4
5	2.6512536917050624E-5	0.0	0.0	0.0	1.060501476682025E-4
6	2.6512536917050624E-5	0.0	0.0	0.0	1.193064161267278E-4
7	3.976880537557594E-5	0.0	0.0	0.0	1.3256268458525312E-4
8	3.976880537557594E-5	0.0	0.0	0.0	1.4581895304377842E-4
9	5.302507383410125E-5	0.0	0.0	0.0	2.12100295336405E-4
10	5.302507383410125E-5	0.0	0.0	0.0	2.386128322534556E-4
11	9.279387920967718E-5	0.0	0.0	0.0	2.386128322534556E-4
12	9.279387920967718E-5	0.0	0.0	2.6512536917050624E-5	2.518691007119809E-4
13	9.279387920967718E-5	0.0	0.0	2.6512536917050624E-5	2.7838163762903157E-4
14	9.279387920967718E-5	0.0	0.0	5.302507383410125E-5	3.579192483801834E-4
15	9.279387920967718E-5	0.0	0.0	1.4581895304377842E-4	3.8443178529723405E-4
16	1.3256268458525312E-4	0.0	0.0	2.7838163762903157E-4	5.169944698824872E-4
17	1.3256268458525312E-4	0.0	0.0	4.3745685913133527E-4	5.169944698824872E-4
18	1.3256268458525312E-4	0.0	0.0	5.832758121751137E-4	6.760696913847908E-4
19	1.3256268458525312E-4	0.0	0.0	8.218886444285693E-4	6.760696913847908E-4
20	1.4581895304377842E-4	0.0	0.0	0.0012063204297258034	7.025822283018415E-4
21	1.4581895304377842E-4	0.0	0.0	0.0019486714634032209	7.158384967603668E-4
22	1.4581895304377842E-4	0.0	0.0	0.0033803484569239544	7.290947652188922E-4
23	1.4581895304377842E-4	0.0	0.0	0.004440849933605979	8.351449128870946E-4
24	1.4581895304377842E-4	0.0	0.0	0.005792989316375561	8.749137182626705E-4
25	1.5907522150230375E-4	0.0	0.0	0.006986053477642839	8.749137182626705E-4
26	1.5907522150230375E-4	0.0	0.0	0.008444243008080624	8.749137182626705E-4
27	1.5907522150230375E-4	0.0	0.0	0.013163474579315635	8.881699867211959E-4
28	1.5907522150230375E-4	0.0	0.0	0.04036533745620957	8.881699867211959E-4
29	1.5907522150230375E-4	0.0	0.0	0.10216606100985458	8.881699867211959E-4
30	1.5907522150230375E-4	0.0	0.0	0.19408502650126908	8.881699867211959E-4
31	1.5907522150230375E-4	0.0	0.0	0.520799018930084	8.881699867211959E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4560	0.0	18.500002	37
TCGTTAC	1195	0.0	17.184101	23
CTCGTTA	1485	0.0	15.946128	22
GAGTACT	11320	0.0	15.460248	11
TCTTATA	7790	0.0	15.460205	37
AGTACTT	11685	0.0	14.977322	12
CGCTACG	670	0.0	14.634329	10
CGTTACG	930	0.0	14.521505	24
GTATTAG	600	0.0	14.491667	1
GTACTTT	12010	0.0	14.4796	13
TACTTTT	12370	0.0	14.118029	14
TATACTG	910	0.0	14.0274725	5
ATACCGT	370	0.0	14.0	6
GTTACGG	1695	0.0	13.970502	25
TACTTAC	1190	0.0	13.680673	31
ACTTTTT	13250	0.0	13.487548	15
TAGACTG	865	0.0	13.473988	5
CGTTACA	550	0.0	13.454545	24
TTATACT	910	0.0	13.417582	4
TACGGAT	2165	0.0	13.159353	27
>>END_MODULE