FastQCFastQC Report
Thu 9 Feb 2017
SRR2727283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4756038
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT326290.6860542325355685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94780.1992835212838922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88110.18525924309267502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71940.15126035578353242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC29050.018.21342537
CGTATAC1452.9867806E-916.5862063
GAGTACT83450.015.78430211
AGTACTT85450.015.41486312
GTACTTT86200.015.25928113
TCTTATA48750.014.91384637
TACTTTT89350.014.80414114
TACTTAC6850.014.31386931
ACTTTTT93900.014.145915
GTACTTA7450.013.9060430
CGCTACG9300.013.72580610
AACGCAG177500.013.6535225
TTAATAC5700.013.6315793
CCTAGCG1907.1431714E-913.6315792
CGACCGA12000.013.25833318
GTATAAT3850.012.9740261
GCTACGG10050.012.885571511
CCGATTT12700.012.81889721
CAATACT5200.012.8076934
CTTTTTT105650.012.78277316
Produced by FastQC (version 0.11.5)