##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727283.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4756038
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94805823670879	33.0	31.0	34.0	30.0	34.0
2	32.098123059571854	33.0	31.0	34.0	30.0	34.0
3	32.177064396878244	34.0	31.0	34.0	30.0	34.0
4	35.7937911766054	37.0	35.0	37.0	35.0	37.0
5	35.65104610181836	37.0	35.0	37.0	33.0	37.0
6	35.66754744179924	37.0	35.0	37.0	33.0	37.0
7	35.61035908459941	37.0	35.0	37.0	33.0	37.0
8	35.57527925554842	37.0	35.0	37.0	33.0	37.0
9	37.27544544429628	39.0	37.0	39.0	34.0	39.0
10	37.082205819213385	39.0	37.0	39.0	33.0	39.0
11	37.19820657446387	39.0	37.0	39.0	34.0	39.0
12	37.09321014676502	39.0	37.0	39.0	33.0	39.0
13	37.146468131667575	39.0	37.0	39.0	33.0	39.0
14	38.24560379879219	40.0	38.0	41.0	34.0	41.0
15	38.19971181895519	40.0	38.0	41.0	33.0	41.0
16	38.1151136723466	40.0	37.0	41.0	33.0	41.0
17	38.07166721544277	40.0	37.0	41.0	33.0	41.0
18	38.020291890014335	40.0	37.0	41.0	33.0	41.0
19	38.02423151370952	40.0	37.0	41.0	33.0	41.0
20	37.88850194216278	40.0	37.0	41.0	33.0	41.0
21	37.8002318316212	40.0	37.0	41.0	33.0	41.0
22	37.71988638442334	40.0	37.0	41.0	32.0	41.0
23	37.58718517387792	39.0	37.0	41.0	32.0	41.0
24	37.45464123709693	39.0	37.0	41.0	32.0	41.0
25	37.355465200236	39.0	37.0	41.0	31.0	41.0
26	36.94887782645976	39.0	36.0	40.0	31.0	41.0
27	36.698125834991224	39.0	36.0	40.0	30.0	41.0
28	36.507563438307265	39.0	36.0	40.0	30.0	41.0
29	36.43152598864854	39.0	36.0	40.0	30.0	41.0
30	36.32356448792041	39.0	35.0	40.0	30.0	41.0
31	36.08148231784523	38.0	35.0	40.0	29.0	41.0
32	35.961041732635444	38.0	35.0	40.0	28.0	41.0
33	35.74645787102626	38.0	35.0	40.0	27.0	41.0
34	35.56692356116583	38.0	35.0	40.0	26.0	41.0
35	35.3890622825133	38.0	34.0	40.0	26.0	41.0
36	35.21293627174552	38.0	34.0	40.0	25.0	41.0
37	34.9357570313778	38.0	34.0	40.0	24.0	41.0
38	34.809479444865666	38.0	33.0	40.0	24.0	41.0
39	34.610247857565476	38.0	33.0	40.0	23.0	41.0
40	34.454905322455375	38.0	33.0	40.0	23.0	41.0
41	34.28439196659068	38.0	33.0	40.0	22.0	41.0
42	34.05538875004783	38.0	33.0	40.0	21.0	41.0
43	30.655118609228943	34.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	18.0
11	23.0
12	11.0
13	26.0
14	63.0
15	120.0
16	209.0
17	423.0
18	847.0
19	1748.0
20	3526.0
21	6568.0
22	11201.0
23	17215.0
24	25208.0
25	34829.0
26	46408.0
27	60252.0
28	76236.0
29	94828.0
30	116394.0
31	142403.0
32	174474.0
33	215439.0
34	272852.0
35	353525.0
36	491125.0
37	759356.0
38	1225253.0
39	625451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78260602627649	18.175758898478104	12.683750634456665	26.35788444078874
2	19.24236097356665	21.460741062203457	33.47958952388522	25.81730844034467
3	19.271692110113502	21.967654589807733	30.33800402772223	28.422649272356526
4	15.228011214376336	15.221156769563237	32.36782380628582	37.183008209774606
5	15.068992299893313	36.99634023109151	33.29727390739939	14.637393561615783
6	37.45258132924926	34.56877342022919	13.247455129668856	14.731190120852695
7	30.838967224399806	30.499167584447388	19.469272533146288	19.192592658006514
8	29.33714995548816	34.39690767819769	17.90334728191827	18.362595084395878
9	27.69660376977644	13.32127707978784	17.12698679026534	41.85513236017038
10	16.09150305359209	27.526209841048367	32.48329807289176	23.89898903246778
11	38.32555585131994	20.781667429906996	19.846981878614088	21.045794840158973
12	22.451187311791873	24.824654470801118	28.706246670022402	24.01791154738461
13	33.14073604962786	18.80540062968378	22.851184115854416	25.202679204833938
14	23.169558359289812	19.657916946836842	24.86605447643606	32.30647021743729
15	26.858237886240605	27.309495845071048	20.858033514450472	24.974232754237878
16	25.695421272916658	25.299692727434053	23.14163595833339	25.8632500413159
17	25.417038299525778	25.56501861423311	24.19783021077628	24.82011287546483
18	25.205202313354096	24.66077857241679	25.052596299693146	25.081422814535966
19	26.54787451235671	24.898539498633106	24.57082975367312	23.98275623533706
20	25.5384418711541	24.642170647080615	24.661072935077474	25.15831454668781
21	25.21283471662758	25.23516422703099	24.680753181534715	24.87124787480672
22	26.056015532256048	24.66033702842576	23.762236550675162	25.521410888643025
23	25.461571164906587	24.550707963224852	24.257291468234694	25.730429403633863
24	25.26830105226241	24.647616356303292	24.16807855614274	25.91600403529156
25	25.580767016579763	24.131178094035413	24.329872889997937	25.958181999386888
26	25.15032470304064	24.534602120504502	24.795260256541262	25.519812919913594
27	25.95849738795191	25.00776907165166	24.376108853629848	24.657624686766592
28	25.471032821857186	25.12784801130689	24.336243739011337	25.06487542782459
29	25.19063136165018	25.1592186605742	24.533109281296746	25.117040696478877
30	25.52956893952487	25.122107939423525	24.21814123436356	25.130181886688035
31	25.189874429094132	24.361201487456576	24.524404556902194	25.924519526547098
32	24.801399820606985	24.997571508049347	24.461684284271907	25.73934438707176
33	25.29910400211268	24.473269557560307	24.464648936783096	25.762977503543915
34	25.42172707619241	24.568096386109612	24.92686139177189	25.083315145926083
35	25.188675952547058	24.68479015516697	24.722195238978326	25.404338653307647
36	24.92745011709326	24.058071024663807	25.246707448510712	25.76777140973222
37	24.477748075183587	24.066418308684664	25.454317227911133	26.001516388220615
38	24.570535391012434	23.784755294217582	25.863838766637272	25.78087054813271
39	24.198692272854004	23.6342518709901	26.338183168427165	25.828872687728737
40	24.551401818067898	23.34417849478915	26.800374597511627	25.304045089631327
41	23.934564862602024	23.153746879230148	27.20295758780733	25.708730670360495
42	23.447415685072322	23.34653339607463	27.264500409794874	25.94155050905817
43	23.509189792007547	22.51079575058063	27.174782875998886	26.80523158141293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	281.0
1	349.5
2	418.0
3	1239.0
4	2060.0
5	2060.0
6	2489.0
7	2918.0
8	3022.5
9	3127.0
10	4350.0
11	5573.0
12	5573.0
13	12308.5
14	19044.0
15	33841.0
16	48638.0
17	35220.0
18	21802.0
19	21802.0
20	25679.0
21	29556.0
22	24680.0
23	19804.0
24	24709.5
25	29615.0
26	29615.0
27	36498.5
28	43382.0
29	51247.0
30	59112.0
31	68460.5
32	77809.0
33	77809.0
34	89703.0
35	101597.0
36	119277.5
37	136958.0
38	158718.5
39	180479.0
40	180479.0
41	204892.5
42	229306.0
43	255145.5
44	280985.0
45	311273.0
46	341561.0
47	341561.0
48	374338.5
49	407116.0
50	423941.5
51	440767.0
52	444790.5
53	448814.0
54	448814.0
55	423989.5
56	399165.0
57	374820.5
58	350476.0
59	328120.5
60	305765.0
61	305765.0
62	271150.5
63	236536.0
64	202404.5
65	168273.0
66	142400.0
67	116527.0
68	116527.0
69	97684.0
70	78841.0
71	66395.0
72	53949.0
73	46169.5
74	38390.0
75	38390.0
76	32538.5
77	26687.0
78	22953.5
79	19220.0
80	15801.0
81	12382.0
82	12382.0
83	10043.0
84	7704.0
85	6067.5
86	4431.0
87	4137.5
88	3844.0
89	3844.0
90	2899.0
91	1954.0
92	1359.5
93	765.0
94	522.0
95	279.0
96	279.0
97	181.0
98	83.0
99	64.0
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4756038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.29805748269957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7883785676513	47.43613274068778
2	9.04234644414457	10.362177726701596
3	2.982932214585771	5.127486644949955
4	1.4219670442680972	3.2590379776391134
5	0.8326065114248958	2.3853367876046816
6	0.5354526044467359	1.8408236465310166
7	0.3935448710815923	1.5784549651678268
8	0.28526680076027505	1.3076186838294437
9	0.21021846245927253	1.0840598591324508
>10	1.3727740540061624	14.549859230012007
>50	0.09264142446690829	3.606566851794877
>100	0.037983627082381406	3.9619142503348166
>500	0.002273747672986607	0.8860599358300892
>1k	0.001466932680978352	1.3933575331504304
>5k	1.1001995107316103E-4	0.5354767832002904
>10k+	3.6673317024387014E-5	0.6856363834337509
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32629	0.6860542325355685	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9478	0.1992835212838922	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8811	0.18525924309267502	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7194	0.15126035578353242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.410361733863355E-5
2	2.1025904334658386E-5	0.0	0.0	0.0	8.410361733863355E-5
3	2.1025904334658386E-5	0.0	0.0	0.0	8.410361733863355E-5
4	2.1025904334658386E-5	0.0	0.0	0.0	1.0512952167329193E-4
5	2.1025904334658386E-5	0.0	0.0	0.0	1.0512952167329193E-4
6	2.1025904334658386E-5	0.0	0.0	0.0	1.471813303426087E-4
7	2.1025904334658386E-5	0.0	0.0	2.1025904334658386E-5	1.8923313901192547E-4
8	2.1025904334658386E-5	0.0	0.0	2.1025904334658386E-5	1.8923313901192547E-4
9	4.205180866931677E-5	0.0	0.0	2.1025904334658386E-5	2.5231085201590065E-4
10	6.307771300397516E-5	0.0	0.0	4.205180866931677E-5	2.5231085201590065E-4
11	1.2615542600795033E-4	0.0	0.0	6.307771300397516E-5	2.5231085201590065E-4
12	1.2615542600795033E-4	2.1025904334658386E-5	0.0	1.0512952167329193E-4	2.73336756350559E-4
13	1.2615542600795033E-4	6.307771300397516E-5	0.0	1.682072346772671E-4	2.943626606852174E-4
14	1.471813303426087E-4	6.307771300397516E-5	0.0	2.1025904334658386E-4	4.415439910278261E-4
15	1.471813303426087E-4	6.307771300397516E-5	0.0	3.364144693545342E-4	4.625698953624845E-4
16	1.471813303426087E-4	6.307771300397516E-5	0.0	6.5180303437441E-4	5.256476083664596E-4
17	1.682072346772671E-4	6.307771300397516E-5	0.0	0.0011564247384062113	5.256476083664596E-4
18	1.8923313901192547E-4	6.307771300397516E-5	0.0	0.0013877096860874536	5.887253213704348E-4
19	2.1025904334658386E-4	6.307771300397516E-5	0.0	0.0018713054857845963	5.887253213704348E-4
20	2.1025904334658386E-4	6.307771300397516E-5	0.0	0.0025861862331629814	6.097512257050931E-4
21	2.1025904334658386E-4	6.307771300397516E-5	0.0	0.003931844110581118	6.097512257050931E-4
22	2.3128494768124224E-4	6.307771300397516E-5	0.0	0.006286745396062858	6.307771300397515E-4
23	2.73336756350559E-4	6.307771300397516E-5	0.0	0.008010869551504846	7.148807473783851E-4
24	2.73336756350559E-4	6.307771300397516E-5	0.0	0.010512952167329193	7.148807473783851E-4
25	2.73336756350559E-4	6.307771300397516E-5	0.0	0.012447335366117765	7.148807473783851E-4
26	2.73336756350559E-4	6.307771300397516E-5	0.0	0.01459197760825292	7.148807473783851E-4
27	2.73336756350559E-4	6.307771300397516E-5	0.0	0.02018486816127205	7.148807473783851E-4
28	2.73336756350559E-4	6.307771300397516E-5	0.0	0.04756059560499727	7.359066517130435E-4
29	2.73336756350559E-4	6.307771300397516E-5	0.0	0.11593683650130635	7.359066517130435E-4
30	3.364144693545342E-4	6.307771300397516E-5	0.0	0.21349703261412126	7.359066517130435E-4
31	3.364144693545342E-4	6.307771300397516E-5	0.0	0.5320815350928652	7.359066517130435E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2905	0.0	18.213425	37
CGTATAC	145	2.9867806E-9	16.586206	3
GAGTACT	8345	0.0	15.784302	11
AGTACTT	8545	0.0	15.414863	12
GTACTTT	8620	0.0	15.259281	13
TCTTATA	4875	0.0	14.913846	37
TACTTTT	8935	0.0	14.804141	14
TACTTAC	685	0.0	14.313869	31
ACTTTTT	9390	0.0	14.1459	15
GTACTTA	745	0.0	13.90604	30
CGCTACG	930	0.0	13.725806	10
AACGCAG	17750	0.0	13.653522	5
TTAATAC	570	0.0	13.631579	3
CCTAGCG	190	7.1431714E-9	13.631579	2
CGACCGA	1200	0.0	13.258333	18
GTATAAT	385	0.0	12.974026	1
GCTACGG	1005	0.0	12.8855715	11
CCGATTT	1270	0.0	12.818897	21
CAATACT	520	0.0	12.807693	4
CTTTTTT	10565	0.0	12.782773	16
>>END_MODULE