##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2300008
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28791334638836	33.0	31.0	34.0	30.0	34.0
2	32.425126782167716	34.0	31.0	34.0	31.0	34.0
3	32.518159936835005	34.0	31.0	34.0	31.0	34.0
4	36.05160416833333	37.0	35.0	37.0	35.0	37.0
5	35.85731919193324	37.0	35.0	37.0	35.0	37.0
6	35.883333014493864	37.0	35.0	37.0	35.0	37.0
7	35.84007620843058	37.0	35.0	37.0	35.0	37.0
8	35.801827211035786	37.0	35.0	37.0	35.0	37.0
9	37.63115128295206	39.0	37.0	39.0	35.0	39.0
10	37.46210969700975	39.0	37.0	39.0	35.0	39.0
11	37.570438450648865	39.0	37.0	39.0	35.0	39.0
12	37.50300129390854	39.0	37.0	39.0	35.0	39.0
13	37.58189493253936	39.0	37.0	39.0	35.0	39.0
14	38.77275992083506	40.0	38.0	41.0	35.0	41.0
15	38.692239766122555	40.0	38.0	41.0	35.0	41.0
16	38.660776832080586	40.0	38.0	41.0	35.0	41.0
17	38.61445481928759	40.0	38.0	41.0	34.0	41.0
18	38.60582397974268	40.0	38.0	41.0	34.0	41.0
19	38.660867701329735	40.0	38.0	41.0	34.0	41.0
20	38.624760870396976	40.0	38.0	41.0	34.0	41.0
21	38.5775997300879	40.0	38.0	41.0	34.0	41.0
22	38.529452071471056	40.0	38.0	41.0	34.0	41.0
23	38.45423885482138	40.0	38.0	41.0	34.0	41.0
24	38.41153987290479	40.0	38.0	41.0	34.0	41.0
25	38.287448130615196	40.0	38.0	41.0	34.0	41.0
26	38.16136161265526	40.0	38.0	41.0	34.0	41.0
27	38.05733240927858	40.0	37.0	41.0	33.0	41.0
28	37.94405410763789	40.0	37.0	41.0	33.0	41.0
29	37.831357108323104	40.0	37.0	41.0	33.0	41.0
30	37.74043351153561	40.0	37.0	41.0	33.0	41.0
31	37.604318767586896	40.0	37.0	41.0	33.0	41.0
32	37.485183529796416	40.0	36.0	41.0	33.0	41.0
33	37.3589609253533	39.0	36.0	41.0	32.0	41.0
34	37.229582679712415	39.0	36.0	41.0	32.0	41.0
35	37.13116041335508	39.0	36.0	41.0	31.0	41.0
36	37.005454763635605	39.0	36.0	41.0	31.0	41.0
37	36.88601735298312	39.0	35.0	41.0	31.0	41.0
38	36.763376909993355	39.0	35.0	41.0	31.0	41.0
39	36.618912629869115	39.0	35.0	41.0	30.0	41.0
40	36.45986840045774	39.0	35.0	40.0	30.0	41.0
41	36.30558111102222	39.0	35.0	40.0	30.0	41.0
42	36.14511427786338	39.0	35.0	40.0	29.0	41.0
43	34.48407222931399	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	11.0
11	13.0
12	11.0
13	9.0
14	6.0
15	9.0
16	10.0
17	26.0
18	67.0
19	117.0
20	320.0
21	661.0
22	1163.0
23	2119.0
24	3672.0
25	5961.0
26	9261.0
27	13832.0
28	19781.0
29	27320.0
30	36332.0
31	46428.0
32	58479.0
33	75601.0
34	100816.0
35	137853.0
36	202476.0
37	338852.0
38	608289.0
39	610502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72123575222347	17.433243710456658	12.994867843937936	24.850652693381935
2	16.34894313411084	21.97161922915051	36.9236976567038	24.755739980034853
3	18.78049989391341	23.367962198392352	30.998109571792792	26.85342833590144
4	13.299171133317797	15.044860713527953	35.03518248632179	36.62078566683246
5	14.407471626185647	37.765216468812284	34.209402749903475	13.61790915509859
6	34.795270277320775	35.964309689357606	14.833861447438442	14.40655858588318
7	28.79368245675667	31.275499911304657	21.58996838271867	18.340849249220003
8	27.858468318371067	36.20952622773486	19.405715110556137	16.526290343337934
9	26.560125008260844	13.989646992532201	18.930151547298966	40.520076451908
10	14.872774355567458	28.37881433455884	34.239750470433144	22.50866083944056
11	36.106570064104126	21.696141926462865	21.758663448127137	20.438624561305872
12	20.77149296872011	25.82073627569991	31.62249870435233	21.785272051227647
13	31.780063373692613	19.132542147679487	25.241694811496306	23.84569966713159
14	21.39953426248952	19.819365845684015	28.533596404882072	30.247503486944392
15	24.96921749837392	28.48594439671514	23.31057109366576	23.234267011245176
16	23.233310492833066	26.405864675253305	25.772475573998005	24.588349257915628
17	23.16039770296451	27.027688599344003	27.265079077985817	22.54683461970567
18	22.97365922205488	25.948344527497298	28.109163098563133	22.968833151884688
19	23.990177425469824	26.465516641681248	27.898859482227888	21.64544645062104
20	23.064832817972807	26.31864758731274	27.814816296291145	22.80170329842331
21	22.52666077683208	27.057514582557975	27.999554784157272	22.416269856452672
22	23.252701729733115	26.26221300099826	27.21307925885475	23.272006010413875
23	22.533139015168643	26.181126326517123	27.827816251073912	23.45791840724032
24	22.57600843127502	26.08430057634582	28.017380809110232	23.322310183268925
25	23.00544172020271	26.083170145495142	27.674555914588122	23.236832219714017
26	22.638356040500728	26.43190806292848	28.28372770877319	22.64600818779761
27	23.29391897767312	26.8853412683782	27.77664251602603	22.04409723792265
28	22.950833214493166	27.16560116312639	27.39368732630495	22.489878296075492
29	22.414878556944153	27.154775113825693	28.040728553987638	22.38961777524252
30	22.79844244020021	26.96677576773646	27.81120761319091	22.42357417887242
31	22.437400217738375	26.438647169922884	28.004163463779257	23.119789148559484
32	21.996227839207517	26.940384555184156	27.994380889109948	23.06900671649838
33	22.560356311804135	26.378821291056383	27.773294701583644	23.28752769555584
34	22.701225387042133	26.544690279338155	28.264988643517764	22.489095690101948
35	22.533139015168643	26.575907562060653	28.020076451907993	22.87087697086271
36	22.313748473918352	26.260126051735472	28.394118629152594	23.03200684519358
37	21.802185035878136	25.99438784560749	28.510639962991434	23.692787155522936
38	22.06018413848995	25.946344534453793	28.732030497285226	23.261440829771026
39	21.602403122076097	25.859388315171078	29.319071933662837	23.219136629089988
40	22.07227105297025	25.811605872675226	29.26715907075106	22.848964003603466
41	21.609968313153693	25.59721531403369	29.39759339967513	23.395222973137486
42	21.030405111634394	25.892605590937073	29.423028093815322	23.653961203613207
43	20.732406148152528	25.40112903955117	29.240289598992696	24.626175213303604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	415.0
1	583.0
2	751.0
3	3190.0
4	5629.0
5	5629.0
6	7367.5
7	9106.0
8	8281.0
9	7456.0
10	9601.0
11	11746.0
12	11746.0
13	18315.0
14	24884.0
15	38769.0
16	52654.0
17	41225.5
18	29797.0
19	29797.0
20	31198.0
21	32599.0
22	25836.0
23	19073.0
24	21882.5
25	24692.0
26	24692.0
27	29186.0
28	33680.0
29	37969.0
30	42258.0
31	47289.5
32	52321.0
33	52321.0
34	58931.5
35	65542.0
36	74631.5
37	83721.0
38	94280.0
39	104839.0
40	104839.0
41	116505.0
42	128171.0
43	139912.5
44	151654.0
45	163619.0
46	175584.0
47	175584.0
48	186536.0
49	197488.0
50	200564.5
51	203641.0
52	201276.5
53	198912.0
54	198912.0
55	183422.0
56	167932.0
57	151193.5
58	134455.0
59	122683.0
60	110911.0
61	110911.0
62	96334.0
63	81757.0
64	68225.5
65	54694.0
66	45495.0
67	36296.0
68	36296.0
69	29532.0
70	22768.0
71	18570.0
72	14372.0
73	11518.0
74	8664.0
75	8664.0
76	6828.0
77	4992.0
78	4020.0
79	3048.0
80	2398.5
81	1749.0
82	1749.0
83	1338.0
84	927.0
85	709.0
86	491.0
87	357.5
88	224.0
89	224.0
90	150.5
91	77.0
92	51.5
93	26.0
94	17.5
95	9.0
96	9.0
97	5.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2300008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.86044867535101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.67978207111216	49.49248851157015
2	9.41668016582988	11.273733995177109
3	2.967316556020818	5.328747012156105
4	1.450543248505025	3.473206787140479
5	0.8370310721258658	2.505252776633221
6	0.5459861664958232	1.9609786138184926
7	0.37653111656767213	1.5777525104580223
8	0.27384500574043996	1.3113987928901447
9	0.2107317383707404	1.1353046768108281
>10	1.1563369247885873	12.135269771316995
>50	0.05449640510188836	2.2140774375976693
>100	0.027454922790545712	3.005436343872167
>500	0.0021038575548083045	0.8963301283420892
>1k	8.70561746817121E-4	1.1501166239830292
>5k	7.254681223476009E-5	0.34289838630758185
>10k+	2.1764043670428025E-4	2.1970076319258958
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29652	1.2892129070855405	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10625	0.4619549149394263	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10314	0.448433222840964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7896	0.34330315372816095	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3889	0.16908636839524038	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3687	0.1603037902476861	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3051	0.13265171251578253	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2696	0.11721698359310054	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	2534	0.11017352983120059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.347810964135777E-5	0.0
2	0.0	0.0	0.0	4.347810964135777E-5	0.0
3	0.0	0.0	0.0	4.347810964135777E-5	0.0
4	0.0	0.0	0.0	4.347810964135777E-5	0.0
5	0.0	0.0	0.0	4.347810964135777E-5	0.0
6	0.0	0.0	0.0	4.347810964135777E-5	4.347810964135777E-5
7	0.0	0.0	0.0	4.347810964135777E-5	4.347810964135777E-5
8	0.0	0.0	0.0	4.347810964135777E-5	4.347810964135777E-5
9	4.347810964135777E-5	0.0	0.0	8.695621928271553E-5	4.347810964135777E-5
10	8.695621928271553E-5	0.0	0.0	1.7391243856543107E-4	4.347810964135777E-5
11	8.695621928271553E-5	0.0	0.0	3.043467674895044E-4	4.347810964135777E-5
12	8.695621928271553E-5	0.0	0.0	7.391278639030821E-4	8.695621928271553E-5
13	8.695621928271553E-5	0.0	0.0	9.565184121098709E-4	1.304343289240733E-4
14	8.695621928271553E-5	0.0	0.0	0.0010434746313925864	1.304343289240733E-4
15	8.695621928271553E-5	0.0	0.0	0.0014782557278061642	1.304343289240733E-4
16	8.695621928271553E-5	0.0	0.0	0.002695642797764182	1.7391243856543107E-4
17	8.695621928271553E-5	0.0	0.0	0.0034347706616672637	1.7391243856543107E-4
18	8.695621928271553E-5	0.0	0.0	0.003739117429156768	2.1739054820678883E-4
19	8.695621928271553E-5	0.0	0.0	0.005086938828038859	2.1739054820678883E-4
20	8.695621928271553E-5	0.0	0.0	0.0073043224197481054	2.1739054820678883E-4
21	8.695621928271553E-5	0.0	0.0	0.010304311985001792	2.1739054820678883E-4
22	8.695621928271553E-5	0.0	0.0	0.01769559062403261	2.1739054820678883E-4
23	8.695621928271553E-5	0.0	0.0	0.02199992347852703	2.1739054820678883E-4
24	8.695621928271553E-5	0.0	0.0	0.03195641058639796	2.1739054820678883E-4
25	8.695621928271553E-5	0.0	0.0	0.038434648922960266	2.1739054820678883E-4
26	8.695621928271553E-5	0.0	0.0	0.04656505542589417	2.1739054820678883E-4
27	8.695621928271553E-5	0.0	0.0	0.06686933262840825	2.1739054820678883E-4
28	8.695621928271553E-5	0.0	0.0	0.18508631274326	2.1739054820678883E-4
29	8.695621928271553E-5	0.0	0.0	0.4042594634453445	2.1739054820678883E-4
30	1.304343289240733E-4	0.0	0.0	0.6643020372103053	2.1739054820678883E-4
31	1.304343289240733E-4	0.0	0.0	1.2345174451567125	2.1739054820678883E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACG	410	0.0	18.500002	24
TCGTTAC	435	0.0	17.43678	23
TAATCGA	85	2.7250355E-5	17.411764	20
CTCGTTA	410	0.0	17.146343	22
CTATAGT	150	4.6784407E-9	16.033333	4
CTTATAC	3195	0.0	14.938966	37
AACGCAG	12210	0.0	14.666667	5
ATAAGAC	205	9.276846E-11	14.439025	3
TATACCG	90	8.2784577E-4	14.388888	5
TATACAC	1350	0.0	14.114815	37
ATGGTTG	1225	0.0	14.044897	32
AGGGCGT	635	0.0	13.984252	10
ACGGATG	810	0.0	13.932098	28
CGTGACG	80	0.0063011516	13.875	10
TAAGACT	320	0.0	13.875	4
GGCGTCA	565	0.0	13.752213	12
TATACTG	350	0.0	13.742857	5
AGTACTG	540	0.0	13.703703	5
TACGGAT	845	0.0	13.573964	27
GGACCGT	165	2.59819E-7	13.454545	6
>>END_MODULE