FastQCFastQC Report
Thu 9 Feb 2017
SRR2727281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084481
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT421720.59527296353819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133090.18786132675068223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126520.17858753520547235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104840.14798543464228361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC48500.019.75876237
AACGCAG167700.017.2754925
ACGCAGA190250.015.2667546
GAGTACT115400.015.19757511
TCTTATA81400.014.79545537
CGCAGAG196100.014.7547177
AGAGTAC198350.014.6339810
AGTACTT120550.014.53297312
CAGAGTA202600.014.4639679
GCAGAGT208700.014.06780058
GTACTTT125750.013.8731613
TCGTTAC8250.013.67878723
GGACCGT5650.013.4247796
TAGATCG3600.013.3611115
TACTTTT130600.013.32963414
TATACCG2800.013.2142865
CTCGTTA8850.013.16949122
ACTTTTT137200.012.80976715
CAACGCA225750.012.7922494
TCAACGC227550.012.6829273
Produced by FastQC (version 0.11.5)