FastQCFastQC Report
Thu 9 Feb 2017
SRR2727280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828882
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.12474634507686257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAT358.867298E-426.42857215
TAATAGA400.001930735523.1254
ATAGGAC851.2444507E-619.5882363
GTTCTAA701.2186764E-418.51
GTATTGA701.2186764E-418.51
TAAGGGT953.6039328E-617.5263164
ATAGCAC650.001579431117.0769233
TTATACT650.001579431117.0769234
TATACTG1005.8738115E-616.6500025
GGGTATA700.002591675115.8571431
TACTTAC855.3619104E-415.23529431
TGTATAC855.3619104E-415.2352943
TGGACCG1101.4508176E-515.1363645
CTCTTAT7650.014.993463537
TCTTATA4300.014.62790737
TAACACT1405.988477E-714.5357154
CTTATAC2550.014.50980437
GGACCGT1152.2082633E-514.4782616
TGTACTG2301.8189894E-1214.4782615
TATACAC1152.2082633E-514.47826137
Produced by FastQC (version 0.11.5)