FastQCFastQC Report
Thu 9 Feb 2017
SRR2727279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986479
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39060.13078946813287487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12300.016.84552837
TAAACGT803.3848308E-416.18754
TCTTATA20350.014.72727337
TCTATAG2059.276846E-1114.4390253
CTCTATA1702.4410838E-814.1470592
TCGTTAC4000.013.87500123
CTCTTAT33700.013.77893137
TATAGAT1652.5990812E-713.4545452
TATACTG2355.638867E-1113.3829795
ATCTATA1551.8901974E-613.1290322
TTACACA4850.012.9690724
TCTATAC2001.465196E-812.9500013
CCCCTAT3350.012.7014921
CTCGTTA4400.012.61363622
TAGAACA3150.012.3333334
GTATAGA2552.237357E-1012.3333331
TAGTACG1653.8087037E-612.3333332
TATACCG1501.9458825E-512.3333325
TACTTAC2901.2732926E-1112.12068931
TAATACC2307.921699E-912.0652184
Produced by FastQC (version 0.11.5)