##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727279.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2986479
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12039562307319	33.0	31.0	34.0	30.0	34.0
2	32.263493900342176	34.0	31.0	34.0	30.0	34.0
3	32.340872646350434	34.0	31.0	34.0	30.0	34.0
4	35.92810630846559	37.0	35.0	37.0	35.0	37.0
5	35.79601564250075	37.0	35.0	37.0	35.0	37.0
6	35.80655380466429	37.0	35.0	37.0	35.0	37.0
7	35.747059999417374	37.0	35.0	37.0	35.0	37.0
8	35.70604949842272	37.0	35.0	37.0	33.0	37.0
9	37.449023750041434	39.0	37.0	39.0	35.0	39.0
10	37.27261902728932	39.0	37.0	39.0	34.0	39.0
11	37.382573927357264	39.0	37.0	39.0	34.0	39.0
12	37.27063843408911	39.0	37.0	39.0	34.0	39.0
13	37.32685044830384	39.0	37.0	39.0	34.0	39.0
14	38.49531605613165	40.0	38.0	41.0	34.0	41.0
15	38.447896335450544	40.0	38.0	41.0	34.0	41.0
16	38.36167439985347	40.0	38.0	41.0	34.0	41.0
17	38.29971749340946	40.0	38.0	41.0	34.0	41.0
18	38.22752646176317	40.0	38.0	41.0	33.0	41.0
19	38.21795264590844	40.0	38.0	41.0	34.0	41.0
20	38.08113500881807	40.0	38.0	41.0	33.0	41.0
21	37.98563693232064	40.0	37.0	41.0	33.0	41.0
22	37.90212789040204	40.0	37.0	41.0	33.0	41.0
23	37.76926641707509	40.0	37.0	41.0	32.0	41.0
24	37.63325508064848	40.0	37.0	41.0	32.0	41.0
25	37.52773885234083	39.0	37.0	41.0	32.0	41.0
26	37.118480993839235	39.0	36.0	41.0	31.0	41.0
27	36.87743560225938	39.0	36.0	40.0	30.0	41.0
28	36.68995763907933	39.0	36.0	40.0	30.0	41.0
29	36.64039392207345	39.0	36.0	40.0	30.0	41.0
30	36.55325351358573	39.0	36.0	40.0	30.0	41.0
31	36.32918128672594	39.0	35.0	40.0	29.0	41.0
32	36.20025119881975	39.0	35.0	40.0	29.0	41.0
33	35.99677010955041	39.0	35.0	40.0	27.0	41.0
34	35.83698897598141	38.0	35.0	40.0	27.0	41.0
35	35.66464020004828	38.0	35.0	40.0	26.0	41.0
36	35.492513424671664	38.0	35.0	40.0	26.0	41.0
37	35.23560520599676	38.0	34.0	40.0	25.0	41.0
38	35.10328182451643	38.0	34.0	40.0	24.0	41.0
39	34.92268788764294	38.0	34.0	40.0	24.0	41.0
40	34.78047928681233	38.0	33.0	40.0	24.0	41.0
41	34.625603595404485	38.0	33.0	40.0	23.0	41.0
42	34.42945455166435	38.0	33.0	40.0	23.0	41.0
43	30.78823457322151	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	10.0
11	14.0
12	9.0
13	16.0
14	27.0
15	51.0
16	108.0
17	264.0
18	513.0
19	1047.0
20	2124.0
21	3875.0
22	6425.0
23	10231.0
24	14916.0
25	20571.0
26	27509.0
27	35403.0
28	44495.0
29	54885.0
30	68098.0
31	83418.0
32	102063.0
33	125819.0
34	159508.0
35	208187.0
36	287107.0
37	436333.0
38	776642.0
39	516810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93407755420346	16.294405552491746	11.379018570028451	28.39249832327634
2	20.542987243506484	19.188415522091397	32.10673840331708	28.161858831085034
3	19.865466993071106	19.82776373113623	28.84892209186805	31.457847183924613
4	16.288244451074323	14.369831497224657	30.53984307272879	38.80208097897223
5	16.09299780778636	34.931636887451745	32.84858189192022	16.12678341284168
6	39.727217234743655	31.9728683844755	12.92495276209878	15.374961618682068
7	32.62771979980438	28.72020864703887	18.392863301566827	20.259208251589918
8	29.915562774759174	32.54819471357408	17.59449840430822	19.94174410735853
9	28.059229614539394	12.445491831685406	16.23587508902624	43.25940346474896
10	16.849607849243206	25.74235412336735	32.03300609178903	25.37503193560042
11	39.909505474506936	19.05504776695232	19.136113128536984	21.899333630003763
12	23.488429016242872	22.98834178977987	27.524452708356563	25.998776485620695
13	33.983530438352325	17.706536694214154	21.464038421164187	26.845894446269337
14	24.276045470267828	18.186834730798378	22.77933312104321	34.757786677890586
15	28.17642447845774	25.337060799690875	19.488133015500864	26.99838170635052
16	27.59855334659979	23.747597086736587	21.644016247895934	27.009833318767683
17	26.911657507050947	23.75164868060348	22.56677512214216	26.769918690203415
18	26.863272770376085	23.33774990549071	23.076673232927472	26.72230409120573
19	28.082769040063564	23.217374038123154	22.720032519900524	25.979824401912754
20	27.503859896553767	22.80709156166844	22.72813570763431	26.960912834143485
21	27.232470075965708	23.14789422594299	22.682195320978316	26.93744037711298
22	27.95234789864586	22.652327372802556	22.05001274075592	27.34531198779566
23	27.297396030576476	22.680755498364462	22.43337388275625	27.588474588302812
24	27.159407449374328	22.600426790210143	22.40554847363735	27.834617286778172
25	27.586532501986454	22.177554236945916	22.449680710964316	27.786232550103318
26	27.180301619398627	22.589075630533483	22.75860637225308	27.47201637781481
27	27.78214747198959	22.74792489751309	22.430494237528542	27.039433392968775
28	27.302686541576215	23.033478554511852	22.41241274423828	27.25142215967365
29	27.179799355696122	23.104029862590696	22.654336427612584	27.0618343541006
30	27.49126981974425	22.916551564568174	22.421721364858083	27.17045725082949
31	27.362020626965734	22.380200898784153	22.593261161387705	27.6645173128624
32	27.00514552421095	22.795506012263942	22.639770780239875	27.559577683285237
33	27.131113260799754	22.41703357030135	22.654972628302424	27.79688054059647
34	27.358538265295017	22.400927647574285	22.984658522628152	27.255875564502546
35	27.11989603811043	22.505699855917285	23.05099081560594	27.32341329036635
36	26.849242870952715	22.03106065704798	23.434519378840434	27.68517709315887
37	26.660559140044178	21.916209690408003	23.67024847655048	27.752982692997342
38	26.484398517451485	21.82225289379232	23.96136051852365	27.73198807023254
39	26.122701683152638	21.69625167295668	24.54033663052712	27.640710013363563
40	26.568142618782854	21.364188397105753	24.809449522330475	27.258219461780914
41	25.963350152470515	21.466717160910893	25.029407539781793	27.540525146836792
42	25.582399876242224	21.367536821789137	25.261821697055293	27.788241604913345
43	25.830786019255452	20.472770777895978	25.07089452160889	28.62554868123968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	56.5
2	77.0
3	201.0
4	325.0
5	325.0
6	361.5
7	398.0
8	397.5
9	397.0
10	549.5
11	702.0
12	702.0
13	1497.5
14	2293.0
15	3931.5
16	5570.0
17	4078.0
18	2586.0
19	2586.0
20	3166.0
21	3746.0
22	3317.0
23	2888.0
24	3810.5
25	4733.0
26	4733.0
27	6422.0
28	8111.0
29	10233.0
30	12355.0
31	16151.5
32	19948.0
33	19948.0
34	25536.0
35	31124.0
36	39702.0
37	48280.0
38	61761.0
39	75242.0
40	75242.0
41	91405.5
42	107569.0
43	128160.5
44	148752.0
45	173086.5
46	197421.0
47	197421.0
48	224879.5
49	252338.0
50	272723.0
51	293108.0
52	302750.0
53	312392.0
54	312392.0
55	304347.0
56	296302.0
57	284227.0
58	272152.0
59	256248.0
60	240344.0
61	240344.0
62	216141.0
63	191938.0
64	166638.5
65	141339.0
66	120749.5
67	100160.0
68	100160.0
69	84420.0
70	68680.0
71	58713.5
72	48747.0
73	41212.0
74	33677.0
75	33677.0
76	28415.5
77	23154.0
78	19438.5
79	15723.0
80	12704.0
81	9685.0
82	9685.0
83	7833.0
84	5981.0
85	4848.5
86	3716.0
87	3043.5
88	2371.0
89	2371.0
90	1770.0
91	1169.0
92	866.0
93	563.0
94	402.0
95	241.0
96	241.0
97	170.0
98	99.0
99	73.0
100	47.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2986479.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.766048721362566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.08449358633389	51.31800878843234
2	8.906040923630297	11.001819152067956
3	2.9782851256492564	5.5187071253088416
4	1.426867122482224	3.5252777682458976
5	0.8175701773477798	2.5249039703597997
6	0.5443610056806517	2.017381703932863
7	0.37697196428795055	1.629884809895811
8	0.28093050985460766	1.3881574047197536
9	0.21502910536462805	1.195334837861636
>10	1.279295960890809	14.24046893285713
>50	0.06848623597437171	2.861406373488479
>100	0.020690291010434736	2.1449160440460338
>500	5.976614681164583E-4	0.24115685119215058
>1k	3.803300248389976E-4	0.39257623759117305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3906	0.13078946813287487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.348424683381333E-5
2	0.0	0.0	0.0	0.0	3.348424683381333E-5
3	0.0	0.0	0.0	0.0	3.348424683381333E-5
4	0.0	0.0	0.0	0.0	3.348424683381333E-5
5	0.0	0.0	0.0	0.0	3.348424683381333E-5
6	0.0	0.0	0.0	0.0	6.696849366762666E-5
7	3.348424683381333E-5	0.0	0.0	0.0	1.0045274050143999E-4
8	1.0045274050143999E-4	0.0	0.0	0.0	1.3393698733525332E-4
9	1.0045274050143999E-4	0.0	0.0	0.0	3.348424683381333E-4
10	1.0045274050143999E-4	0.0	0.0	0.0	3.683267151719466E-4
11	1.0045274050143999E-4	0.0	0.0	0.0	3.683267151719466E-4
12	1.0045274050143999E-4	0.0	0.0	0.0	4.352952088395733E-4
13	1.0045274050143999E-4	0.0	0.0	0.0	5.357479493410133E-4
14	1.0045274050143999E-4	0.0	0.0	0.0	6.362006898424533E-4
15	1.0045274050143999E-4	0.0	0.0	0.0	7.701376771777065E-4
16	1.3393698733525332E-4	0.0	0.0	0.0	9.710431581805866E-4
17	1.3393698733525332E-4	0.0	0.0	2.343897278366933E-4	9.710431581805866E-4
18	1.3393698733525332E-4	0.0	0.0	2.6787397467050665E-4	9.710431581805866E-4
19	1.3393698733525332E-4	0.0	0.0	4.352952088395733E-4	9.710431581805866E-4
20	1.6742123416906666E-4	0.0	0.0	5.357479493410133E-4	0.0010045274050144
21	1.6742123416906666E-4	0.0	0.0	9.040746645129599E-4	0.0011049801455158398
22	1.6742123416906666E-4	0.0	0.0	0.00140633836702016	0.0011049801455158398
23	1.6742123416906666E-4	0.0	0.0	0.0017076965885244799	0.0012724013796849066
24	1.6742123416906666E-4	0.0	0.0	0.0022434445378654933	0.0013058856265187198
25	1.6742123416906666E-4	0.0	0.0	0.0023773815252007466	0.0013728541201863465
26	1.6742123416906666E-4	0.0	0.0	0.00311403495554464	0.00140633836702016
27	1.6742123416906666E-4	0.0	0.0	0.006395491145258346	0.0014733068606877864
28	1.6742123416906666E-4	0.0	0.0	0.02625164951770965	0.0014733068606877864
29	1.6742123416906666E-4	0.0	0.0	0.07269429987620873	0.0014733068606877864
30	1.6742123416906666E-4	0.0	0.0	0.1401650572463426	0.0014733068606877864
31	1.6742123416906666E-4	0.0	0.0	0.3156894791491921	0.0014733068606877864
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1230	0.0	16.845528	37
TAAACGT	80	3.3848308E-4	16.1875	4
TCTTATA	2035	0.0	14.727273	37
TCTATAG	205	9.276846E-11	14.439025	3
CTCTATA	170	2.4410838E-8	14.147059	2
TCGTTAC	400	0.0	13.875001	23
CTCTTAT	3370	0.0	13.778931	37
TATAGAT	165	2.5990812E-7	13.454545	2
TATACTG	235	5.638867E-11	13.382979	5
ATCTATA	155	1.8901974E-6	13.129032	2
TTACACA	485	0.0	12.969072	4
TCTATAC	200	1.465196E-8	12.950001	3
CCCCTAT	335	0.0	12.701492	1
CTCGTTA	440	0.0	12.613636	22
TAGAACA	315	0.0	12.333333	4
GTATAGA	255	2.237357E-10	12.333333	1
TAGTACG	165	3.8087037E-6	12.333333	2
TATACCG	150	1.9458825E-5	12.333332	5
TACTTAC	290	1.2732926E-11	12.120689	31
TAATACC	230	7.921699E-9	12.065218	4
>>END_MODULE