FastQCFastQC Report
Thu 9 Feb 2017
SRR2727278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743254
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT274391.0002354867613426No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95730.3489651341071589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86070.3137514790828702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60310.21984839901810038No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA32850.1197482989180003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13200.016.53787837
TAGTACT2600.016.3653854
TATACCG1059.352469E-615.8571425
TAGATCG957.065381E-515.5789485
TTAGTAC1201.937784E-615.4166673
TTAATAC2650.015.3584913
GAGTACT74600.014.23458411
AGTACTT75600.014.1441812
TAATACT2750.014.1272744
CGCAATC1051.656952E-414.09523822
TCTTATA21900.014.02283137
AACGCAG113350.013.7587135
GTCTTAG4550.013.4175821
CTCTTAT35750.013.40279737
GTACTTT78900.013.3650213
CGAACGT1254.849169E-513.324
GTACTAA1703.736568E-713.0588241
TACTTTT80650.013.00619914
GTAATAC2152.904926E-912.9069773
CTAGTAC1755.3123404E-712.6857153
Produced by FastQC (version 0.11.5)