##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727278.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2743254
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.120125223548385	33.0	31.0	34.0	30.0	34.0
2	32.2597130998442	33.0	31.0	34.0	30.0	34.0
3	32.33782106943068	34.0	31.0	34.0	30.0	34.0
4	35.909855594851955	37.0	35.0	37.0	35.0	37.0
5	35.795693362699915	37.0	35.0	37.0	35.0	37.0
6	35.82415700478337	37.0	35.0	37.0	35.0	37.0
7	35.76040716608816	37.0	35.0	37.0	35.0	37.0
8	35.73975359190217	37.0	35.0	37.0	35.0	37.0
9	37.45891922512462	39.0	37.0	39.0	35.0	39.0
10	37.304819750559005	39.0	37.0	39.0	34.0	39.0
11	37.41177776465468	39.0	37.0	39.0	35.0	39.0
12	37.32117623814638	39.0	37.0	39.0	34.0	39.0
13	37.375532488059804	39.0	37.0	39.0	34.0	39.0
14	38.51934782561148	40.0	38.0	41.0	34.0	41.0
15	38.48643654579561	40.0	38.0	41.0	34.0	41.0
16	38.415066195109894	40.0	38.0	41.0	34.0	41.0
17	38.39597973793167	40.0	38.0	41.0	34.0	41.0
18	38.366469528523425	40.0	38.0	41.0	34.0	41.0
19	38.376454385922706	40.0	38.0	41.0	34.0	41.0
20	38.26943184991255	40.0	38.0	41.0	34.0	41.0
21	38.19577552789497	40.0	38.0	41.0	34.0	41.0
22	38.11787351809202	40.0	38.0	41.0	33.0	41.0
23	38.00826245036005	40.0	37.0	41.0	33.0	41.0
24	37.896127737351335	40.0	37.0	41.0	33.0	41.0
25	37.81232652900533	40.0	37.0	41.0	33.0	41.0
26	37.44466316279863	39.0	37.0	41.0	32.0	41.0
27	37.218483596487964	39.0	36.0	41.0	31.0	41.0
28	37.07409959121539	39.0	36.0	40.0	31.0	41.0
29	37.00909358010596	39.0	36.0	40.0	31.0	41.0
30	36.91256077636267	39.0	36.0	40.0	31.0	41.0
31	36.692907401210384	39.0	36.0	40.0	30.0	41.0
32	36.56548062993802	39.0	35.0	40.0	30.0	41.0
33	36.379302098894236	39.0	35.0	40.0	30.0	41.0
34	36.23040885021948	39.0	35.0	40.0	30.0	41.0
35	36.06681444736798	38.0	35.0	40.0	29.0	41.0
36	35.903900258598	38.0	35.0	40.0	28.0	41.0
37	35.644612201422106	38.0	35.0	40.0	27.0	41.0
38	35.51969157795815	38.0	35.0	40.0	26.0	41.0
39	35.340672792238706	38.0	34.0	40.0	25.0	41.0
40	35.168324916322	38.0	34.0	40.0	25.0	41.0
41	35.01254131042915	38.0	34.0	40.0	24.0	41.0
42	34.77490782844024	38.0	33.0	40.0	24.0	41.0
43	31.465013083002887	35.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	13.0
11	23.0
12	12.0
13	17.0
14	29.0
15	40.0
16	80.0
17	170.0
18	272.0
19	612.0
20	1276.0
21	2436.0
22	4216.0
23	6972.0
24	10432.0
25	14960.0
26	20690.0
27	27130.0
28	35825.0
29	45479.0
30	56396.0
31	70381.0
32	88276.0
33	110847.0
34	143022.0
35	187636.0
36	265231.0
37	422794.0
38	723835.0
39	504149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03739281889318	18.46741132975656	13.003936201314206	25.491259650036053
2	19.039614997371736	22.08621585897624	33.35021109966485	25.52395804398718
3	19.51463480960932	22.29082687931923	30.368897666785504	27.825640644285947
4	15.255641657680988	15.495612145284396	32.14175573971641	37.106990457318204
5	15.320455196638735	36.87474801822945	32.93373490023162	14.871061884900193
6	36.99949038623474	34.17871622532948	13.61354070749555	15.208252680940227
7	30.253013392124828	30.191881612129244	19.834000059783016	19.72110493596291
8	29.001616328637454	33.99010809790125	18.372888547688255	18.635387025773042
9	26.716847947729228	13.702486171532058	18.174948437148	41.40571744359071
10	16.113382136688763	27.96675043579632	32.77370597108398	23.14616145643094
11	37.222911185037916	21.233432995996726	20.237353157964957	21.306302661000405
12	21.900378164034393	25.4688774717908	29.249351317814536	23.381393046360273
13	32.733498246972395	19.15980073299811	23.219395652024932	24.88730536800457
14	22.887089565895103	20.2807687512713	25.67173874530029	31.16040293753331
15	26.229470548480016	27.821412089438308	21.527572729320728	24.42154463276095
16	24.98427050502797	25.445984950719108	23.91973182213532	25.650012722117605
17	24.569325334074062	26.079575569743085	25.400199908575726	23.950899187607128
18	24.363292644428842	24.726656736853386	26.153830450989958	24.756220167727815
19	25.265651667690996	25.31088991394891	25.904564433333555	23.518893985026544
20	24.948254882704994	25.246440905581473	25.725871537961854	24.079432673751683
21	24.61292319267556	25.512037893683924	25.495160127352406	24.37987878628811
22	24.99393056567128	25.14845508290519	25.182137709450163	24.675476641973365
23	24.640773329775513	24.982593664312528	25.184871688877514	25.191761317034437
24	24.605887752282506	25.067456385737525	25.314535219852047	25.01212064212793
25	24.850888762032245	24.92725792070293	25.363819755662437	24.85803356160239
26	24.563055407920668	25.15727672319078	25.74869115291548	24.530976715973075
27	24.868131058954074	25.489765074615768	25.605722255394507	24.036381611035655
28	24.714955304904322	25.47711586313189	25.589974533892963	24.217954298070833
29	24.269681188836323	25.715846946728227	25.817368716130552	24.197103148304898
30	24.304384501034175	25.769323584327225	25.739760153452796	24.186531761185805
31	24.319548973591218	25.142622593460175	25.60473802280066	24.933090410147948
32	24.054717499728426	25.574008094037225	25.60174887196009	24.76952553427426
33	24.47330797658547	25.25657485599219	25.38835266439054	24.8817645030318
34	24.42005005734066	25.21319571574488	25.74548328372072	24.62127094319374
35	24.329792283179028	25.34165629577137	25.75656501366625	24.57198640738335
36	24.12773297696823	24.962836106317535	26.047423971677432	24.862006945036807
37	23.832681917168443	24.656375239040933	26.36190451194093	25.149038331849695
38	23.53599046971225	24.79507912865524	26.805210162821236	24.86372023881128
39	23.408040232512192	24.525326491823215	27.207579028409327	24.859054247255266
40	23.582723291390444	24.515192541412496	27.500333545490136	24.40175062170692
41	23.2788870443641	24.35111732271237	27.63382464766296	24.736170985260568
42	22.98507538857138	24.557915526597245	27.542363922553285	24.91464516227808
43	23.228873447373083	23.837019831193174	27.51064976119601	25.423456960237733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	548.0
1	556.5
2	565.0
3	1429.5
4	2294.0
5	2294.0
6	2587.5
7	2881.0
8	2930.0
9	2979.0
10	4466.0
11	5953.0
12	5953.0
13	12442.5
14	18932.0
15	30488.0
16	42044.0
17	30068.0
18	18092.0
19	18092.0
20	21216.5
21	24341.0
22	19282.5
23	14224.0
24	17480.5
25	20737.0
26	20737.0
27	25499.5
28	30262.0
29	35095.0
30	39928.0
31	45258.0
32	50588.0
33	50588.0
34	57230.5
35	63873.0
36	74181.5
37	84490.0
38	97550.0
39	110610.0
40	110610.0
41	125073.0
42	139536.0
43	153244.0
44	166952.0
45	182506.0
46	198060.0
47	198060.0
48	213994.0
49	229928.0
50	238015.5
51	246103.0
52	248550.5
53	250998.0
54	250998.0
55	237037.5
56	223077.0
57	208370.5
58	193664.0
59	179449.0
60	165234.0
61	165234.0
62	145925.0
63	126616.0
64	107981.0
65	89346.0
66	75082.0
67	60818.0
68	60818.0
69	50690.5
70	40563.0
71	34189.0
72	27815.0
73	23009.0
74	18203.0
75	18203.0
76	15229.0
77	12255.0
78	10234.5
79	8214.0
80	6655.0
81	5096.0
82	5096.0
83	4071.5
84	3047.0
85	2503.5
86	1960.0
87	1626.0
88	1292.0
89	1292.0
90	975.0
91	658.0
92	468.0
93	278.0
94	212.0
95	146.0
96	146.0
97	90.5
98	35.0
99	27.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2743254.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.96183958172594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.63615607045017	50.986139295989766
2	8.64040145035634	10.534695342766707
3	2.8307780831690983	5.177083181928606
4	1.3293675293330696	3.241627602734318
5	0.8037042702596694	2.4497645397359036
6	0.5300068224484433	1.9386114532393386
7	0.3817025460015365	1.6288502564097422
8	0.2701465630919815	1.3174905154214382
9	0.22131152930812112	1.2142402152541307
>10	1.2796267284616687	13.967266226632777
>50	0.05513300660798734	2.2571705702047917
>100	0.01951240488375463	2.0996041492115882
>500	0.0011363032482107917	0.4551842826915774
>1k	7.774706434627201E-4	0.8491912144100392
>5k	1.794163023375508E-4	0.8826965692976757
>10k+	5.980543411251694E-5	1.0003845840716583
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27439	1.0002354867613426	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9573	0.3489651341071589	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8607	0.3137514790828702	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6031	0.21984839901810038	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3285	0.1197482989180003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.645305903135473E-5
2	3.645305903135473E-5	0.0	0.0	0.0	3.645305903135473E-5
3	3.645305903135473E-5	0.0	0.0	0.0	3.645305903135473E-5
4	3.645305903135473E-5	0.0	0.0	0.0	3.645305903135473E-5
5	3.645305903135473E-5	0.0	0.0	0.0	3.645305903135473E-5
6	3.645305903135473E-5	0.0	0.0	0.0	1.093591770940642E-4
7	3.645305903135473E-5	0.0	0.0	0.0	1.4581223612541893E-4
8	3.645305903135473E-5	0.0	0.0	0.0	1.4581223612541893E-4
9	3.645305903135473E-5	0.0	0.0	0.0	1.8226529515677367E-4
10	3.645305903135473E-5	0.0	0.0	0.0	1.8226529515677367E-4
11	3.645305903135473E-5	0.0	0.0	0.0	1.8226529515677367E-4
12	3.645305903135473E-5	0.0	0.0	0.0	1.8226529515677367E-4
13	3.645305903135473E-5	0.0	0.0	0.0	1.8226529515677367E-4
14	3.645305903135473E-5	0.0	0.0	1.093591770940642E-4	2.5517141321948315E-4
15	3.645305903135473E-5	0.0	0.0	2.5517141321948315E-4	2.5517141321948315E-4
16	3.645305903135473E-5	0.0	0.0	3.6453059031354735E-4	2.9162447225083787E-4
17	3.645305903135473E-5	0.0	0.0	5.46795885470321E-4	2.9162447225083787E-4
18	3.645305903135473E-5	0.0	0.0	6.926081215957399E-4	2.9162447225083787E-4
19	7.290611806270947E-5	0.0	0.0	0.001093591770940642	2.9162447225083787E-4
20	7.290611806270947E-5	0.0	0.0	0.0018226529515677366	2.9162447225083787E-4
21	7.290611806270947E-5	0.0	0.0	0.003025603899602443	2.9162447225083787E-4
22	7.290611806270947E-5	0.0	0.0	0.005103428264389663	3.2807753128219263E-4
23	7.290611806270947E-5	0.0	0.0	0.006051207799204886	4.7388976740761154E-4
24	7.290611806270947E-5	0.0	0.0	0.008274844400117524	4.7388976740761154E-4
25	1.093591770940642E-4	0.0	0.0	0.009842325938465778	4.7388976740761154E-4
26	1.093591770940642E-4	0.0	0.0	0.011482713594876742	4.7388976740761154E-4
27	1.093591770940642E-4	0.0	0.0	0.016586141859266403	4.7388976740761154E-4
28	1.093591770940642E-4	0.0	0.0	0.04228554847637149	4.7388976740761154E-4
29	1.093591770940642E-4	0.0	0.0	0.10225083058295004	4.7388976740761154E-4
30	1.093591770940642E-4	0.0	0.0	0.1915972782688005	4.7388976740761154E-4
31	1.093591770940642E-4	0.0	0.0	0.434374651417623	4.7388976740761154E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1320	0.0	16.537878	37
TAGTACT	260	0.0	16.365385	4
TATACCG	105	9.352469E-6	15.857142	5
TAGATCG	95	7.065381E-5	15.578948	5
TTAGTAC	120	1.937784E-6	15.416667	3
TTAATAC	265	0.0	15.358491	3
GAGTACT	7460	0.0	14.234584	11
AGTACTT	7560	0.0	14.14418	12
TAATACT	275	0.0	14.127274	4
CGCAATC	105	1.656952E-4	14.095238	22
TCTTATA	2190	0.0	14.022831	37
AACGCAG	11335	0.0	13.758713	5
GTCTTAG	455	0.0	13.417582	1
CTCTTAT	3575	0.0	13.402797	37
GTACTTT	7890	0.0	13.36502	13
CGAACGT	125	4.849169E-5	13.32	4
GTACTAA	170	3.736568E-7	13.058824	1
TACTTTT	8065	0.0	13.006199	14
GTAATAC	215	2.904926E-9	12.906977	3
CTAGTAC	175	5.3123404E-7	12.685715	3
>>END_MODULE