FastQCFastQC Report
Thu 9 Feb 2017
SRR2727277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5623215
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT366840.6523670178003153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124790.22191931128366957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124250.22095900654696643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93860.16691518997584123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC32950.018.58421737
AACGCAG150450.015.6041875
CTATACT6400.014.74218754
TCTTATA53300.014.1613537
GAGTACT108050.013.88570111
ACGCAGA173550.013.5378856
CTCTTAT82200.013.52615537
AGTACTT111400.013.48473912
ACTATAC4700.013.3829793
GTATACG2102.046363E-913.2142851
CGCAGAG178450.013.1454197
TAGTACG2408.185452E-1113.1041662
GTACTTT115250.013.01822213
AGAGTAC179600.013.00974510
TAGACAG9750.012.9025645
GTATAGA6350.012.8188971
CAGAGTA184000.012.8092399
TCTATAC5600.012.5535713
TACTTTT120050.012.40524814
GCAGAGT192300.012.4006768
Produced by FastQC (version 0.11.5)