##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727277.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5623215
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13566634034089	33.0	31.0	34.0	30.0	34.0
2	32.27717257832041	34.0	31.0	34.0	30.0	34.0
3	32.36255113845016	34.0	31.0	34.0	30.0	34.0
4	35.93446951610422	37.0	35.0	37.0	35.0	37.0
5	35.81472413912682	37.0	35.0	37.0	35.0	37.0
6	35.83592624503954	37.0	35.0	37.0	35.0	37.0
7	35.77799159377687	37.0	35.0	37.0	35.0	37.0
8	35.752193896196395	37.0	35.0	37.0	35.0	37.0
9	37.48200202197497	39.0	37.0	39.0	35.0	39.0
10	37.32015706317471	39.0	37.0	39.0	34.0	39.0
11	37.43371007510828	39.0	37.0	39.0	35.0	39.0
12	37.33885295155885	39.0	37.0	39.0	34.0	39.0
13	37.39693075936097	39.0	37.0	39.0	34.0	39.0
14	38.5655291501392	40.0	38.0	41.0	34.0	41.0
15	38.52828159691564	40.0	38.0	41.0	34.0	41.0
16	38.46288751185932	40.0	38.0	41.0	34.0	41.0
17	38.4275237564276	40.0	38.0	41.0	34.0	41.0
18	38.38423713124965	40.0	38.0	41.0	34.0	41.0
19	38.39242479613531	40.0	38.0	41.0	34.0	41.0
20	38.28129762066718	40.0	38.0	41.0	34.0	41.0
21	38.20685764282533	40.0	38.0	41.0	34.0	41.0
22	38.135589515961954	40.0	38.0	41.0	33.0	41.0
23	38.023213410833485	40.0	38.0	41.0	33.0	41.0
24	37.9199671362379	40.0	37.0	41.0	33.0	41.0
25	37.83374333010564	40.0	37.0	41.0	33.0	41.0
26	37.478716712770186	39.0	37.0	41.0	32.0	41.0
27	37.26987728550304	39.0	37.0	41.0	31.0	41.0
28	37.12100266484565	39.0	36.0	41.0	31.0	41.0
29	37.07245908257109	39.0	36.0	40.0	31.0	41.0
30	36.98931998865418	39.0	36.0	40.0	31.0	41.0
31	36.779785051789766	39.0	36.0	40.0	30.0	41.0
32	36.66718291937975	39.0	36.0	40.0	30.0	41.0
33	36.48629493981646	39.0	35.0	40.0	30.0	41.0
34	36.35331745273834	39.0	35.0	40.0	30.0	41.0
35	36.20194106040761	39.0	35.0	40.0	29.0	41.0
36	36.04803444292989	39.0	35.0	40.0	28.0	41.0
37	35.80955449862756	38.0	35.0	40.0	27.0	41.0
38	35.69574611676772	38.0	35.0	40.0	27.0	41.0
39	35.527859240665705	38.0	35.0	40.0	26.0	41.0
40	35.38349271724449	38.0	34.0	40.0	26.0	41.0
41	35.23873069053913	38.0	34.0	40.0	25.0	41.0
42	35.033172837958354	38.0	34.0	40.0	24.0	41.0
43	31.668619819800593	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	20.0
11	30.0
12	27.0
13	25.0
14	34.0
15	72.0
16	145.0
17	258.0
18	584.0
19	1225.0
20	2456.0
21	4708.0
22	8155.0
23	13291.0
24	20465.0
25	29478.0
26	40378.0
27	54067.0
28	69553.0
29	89248.0
30	112427.0
31	141270.0
32	176453.0
33	222688.0
34	287632.0
35	378968.0
36	534434.0
37	848043.0
38	1515153.0
39	1071918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59554365251906	18.53203194258089	12.67852998684916	26.193894418050885
2	18.78713511754397	21.39052125874611	34.07995248269896	25.742391141010966
3	18.979996318831844	22.059017839438827	30.409027575861852	28.551958265867476
4	15.00611305098596	15.498767164335705	32.92714221312897	36.56797757154937
5	14.832920313379446	36.93589876965401	33.6475130330247	14.583667883941837
6	36.79715251862146	34.771194058914695	13.829864232471994	14.601789189991846
7	30.43091896717447	30.516901807951502	19.940354405798104	19.11182481907592
8	28.54023899139549	34.671678034718575	18.448325379698268	18.339757594187667
9	27.070937177397624	13.575152292772016	17.944556628192235	41.40935390163812
10	15.681847484046049	27.825114280709524	33.32933206359707	23.16370617164736
11	37.50713782062397	21.019114510115656	20.700986890951175	20.772760778309205
12	21.849600273153346	25.035375670323827	29.56991685361488	23.545107202907943
13	32.18006069481604	19.372440854564516	23.381606429773715	25.065892020845727
14	22.733649700393812	20.02182025762842	25.39412062316664	31.850409418811125
15	25.894635008620515	27.903965969645476	21.337259912701185	24.86413910903282
16	25.192918997406288	25.786102789952007	23.671227224994954	25.349750987646747
17	24.62609023485675	26.095801067538765	25.012595107958703	24.26551358964578
18	24.502531025400952	25.221461388191628	25.627545807869694	24.648461778537722
19	25.54504851761848	25.272108571342194	25.48975986157385	23.693083049465475
20	25.0739834774235	25.212658594771852	25.334386111859498	24.37897181594515
21	24.814950166408362	25.35074685922555	25.309613806336767	24.52468916802932
22	25.361665879750284	24.952184826651656	24.83600573693163	24.850143556666428
23	24.921721826392908	24.915248661130686	25.03695839479728	25.12607111767912
24	24.80429789719938	24.927501438234177	25.0423645547965	25.225836109769944
25	25.08504476531664	24.695374443267777	25.14047568872967	25.079105102685915
26	24.689292513268654	24.985688791909965	25.43667634973943	24.888342345081952
27	25.081310246896127	25.281106982393524	25.104126376103352	24.533456394607
28	24.87930836718852	25.34795486212069	25.179279113460893	24.593457657229894
29	24.605763784596533	25.579068202087242	25.292701772918164	24.522466240398064
30	24.848916500613974	25.484798287100887	25.090504275579008	24.57578093670614
31	24.75574915773272	24.865650699822076	25.254005048713235	25.124595093731966
32	24.399316049626414	25.309720506863066	25.247691934240468	25.043271509270053
33	24.654099123010592	24.870718974821344	25.239298159504838	25.23588374266323
34	24.736934298261758	24.779098789571446	25.684755073387734	24.799211838779062
35	24.630447173014012	24.87075454166344	25.552713172091057	24.946085113231486
36	24.39478127725865	24.494457352244222	25.957890637295566	25.15287073320156
37	24.164308140449904	24.206081396496486	26.214736587521553	25.414873875532056
38	23.881178293911933	24.260623148857015	26.660709220614898	25.19748933661615
39	23.680030018414733	24.060381827833368	27.09425124239425	25.16533691135765
40	23.936840401798616	23.912832783381038	27.326040352360703	24.824286462459643
41	23.465348559498437	23.828432667077465	27.620836123107512	25.08538265031659
42	23.14220601559784	24.029705426522018	27.548688783907426	25.279399773972717
43	23.27819583636763	23.311344133204937	27.45971832839399	25.950741702033447
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	874.0
1	935.5
2	997.0
3	2137.0
4	3277.0
5	3277.0
6	3699.5
7	4122.0
8	4175.0
9	4228.0
10	6328.0
11	8428.0
12	8428.0
13	16915.0
14	25402.0
15	41145.5
16	56889.0
17	42404.5
18	27920.0
19	27920.0
20	33406.0
21	38892.0
22	33800.5
23	28709.0
24	35509.5
25	42310.0
26	42310.0
27	52385.5
28	62461.0
29	72708.5
30	82956.0
31	96530.0
32	110104.0
33	110104.0
34	126899.5
35	143695.0
36	166022.5
37	188350.0
38	215210.5
39	242071.0
40	242071.0
41	270167.5
42	298264.0
43	326556.0
44	354848.0
45	384732.5
46	414617.0
47	414617.0
48	443936.0
49	473255.0
50	488400.5
51	503546.0
52	502672.0
53	501798.0
54	501798.0
55	478438.5
56	455079.0
57	427996.0
58	400913.0
59	370251.0
60	339589.0
61	339589.0
62	299409.0
63	259229.0
64	221351.5
65	183474.0
66	154800.0
67	126126.0
68	126126.0
69	104534.5
70	82943.0
71	69840.5
72	56738.0
73	46865.0
74	36992.0
75	36992.0
76	30477.5
77	23963.0
78	20088.0
79	16213.0
80	12948.0
81	9683.0
82	9683.0
83	7843.5
84	6004.0
85	4840.5
86	3677.0
87	3033.0
88	2389.0
89	2389.0
90	1826.5
91	1264.0
92	904.5
93	545.0
94	407.0
95	269.0
96	269.0
97	179.0
98	89.0
99	56.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5623215.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.56104659447848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74999428469071	47.056152301194324
2	9.58743216799165	11.037252594863386
3	3.171282398150572	5.476270016525836
4	1.5155648163770632	3.4894998804972945
5	0.8835164878393374	2.542806686175504
6	0.5904284522775489	2.039140779135228
7	0.4016684039215247	1.6184317599559679
8	0.30436107874697693	1.4015473788240378
9	0.23368262968880746	1.2105915062253936
>10	1.4288120749291022	15.170292385116612
>50	0.10060779170991697	3.9035412549029687
>100	0.031042552015065956	2.953929257692022
>500	0.001050646185046454	0.37402722294941293
>1k	4.3261203736716006E-4	0.46411249399020477
>5k	3.0900859810526825E-5	0.16694745213174828
>10k+	9.270257943158047E-5	1.0954570298199566
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36684	0.6523670178003153	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12479	0.22191931128366957	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12425	0.22095900654696643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9386	0.16691518997584123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.335026315017299E-5
2	0.0	0.0	0.0	0.0	5.335026315017299E-5
3	0.0	0.0	0.0	1.7783421050057662E-5	5.335026315017299E-5
4	0.0	0.0	0.0	1.7783421050057662E-5	5.335026315017299E-5
5	0.0	1.7783421050057662E-5	0.0	1.7783421050057662E-5	5.335026315017299E-5
6	0.0	1.7783421050057662E-5	0.0	1.7783421050057662E-5	1.422673684004613E-4
7	0.0	1.7783421050057662E-5	0.0	1.7783421050057662E-5	1.422673684004613E-4
8	0.0	1.7783421050057662E-5	0.0	1.7783421050057662E-5	1.6005078945051897E-4
9	0.0	1.7783421050057662E-5	0.0	1.7783421050057662E-5	2.1340105260069195E-4
10	0.0	1.7783421050057662E-5	0.0	1.7783421050057662E-5	2.1340105260069195E-4
11	1.7783421050057662E-5	1.7783421050057662E-5	0.0	1.7783421050057662E-5	2.1340105260069195E-4
12	1.7783421050057662E-5	1.7783421050057662E-5	0.0	3.5566842100115325E-5	2.845347368009226E-4
13	1.7783421050057662E-5	1.7783421050057662E-5	0.0	3.5566842100115325E-5	3.0231815785098027E-4
14	1.7783421050057662E-5	1.7783421050057662E-5	0.0	5.335026315017299E-5	3.2010157890103794E-4
15	1.7783421050057662E-5	3.5566842100115325E-5	0.0	1.0670052630034597E-4	3.556684210011533E-4
16	1.7783421050057662E-5	3.5566842100115325E-5	0.0	3.0231815785098027E-4	4.0901868415132623E-4
17	1.7783421050057662E-5	3.5566842100115325E-5	0.0	4.979357894016145E-4	4.0901868415132623E-4
18	1.7783421050057662E-5	3.5566842100115325E-5	0.0	5.868528946519029E-4	4.979357894016145E-4
19	1.7783421050057662E-5	3.5566842100115325E-5	0.0	7.646871051524795E-4	5.157192104516722E-4
20	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.001209272631403921	5.335026315017299E-4
21	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.0019206094734062276	5.512860525517876E-4
22	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.0030231815785098028	5.868528946519029E-4
23	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.0037523018415621667	6.402031578020759E-4
24	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.005086058420316492	6.402031578020759E-4
25	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.005957446051769317	6.402031578020759E-4
26	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.006882183946372316	6.579865788521335E-4
27	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.011612573945687654	6.579865788521335E-4
28	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.03590472710006642	6.579865788521335E-4
29	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.09160240182884702	6.579865788521335E-4
30	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.16858683155454665	6.579865788521335E-4
31	1.7783421050057662E-5	3.5566842100115325E-5	0.0	0.4509164241452621	6.579865788521335E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3295	0.0	18.584217	37
AACGCAG	15045	0.0	15.604187	5
CTATACT	640	0.0	14.7421875	4
TCTTATA	5330	0.0	14.16135	37
GAGTACT	10805	0.0	13.885701	11
ACGCAGA	17355	0.0	13.537885	6
CTCTTAT	8220	0.0	13.526155	37
AGTACTT	11140	0.0	13.484739	12
ACTATAC	470	0.0	13.382979	3
GTATACG	210	2.046363E-9	13.214285	1
CGCAGAG	17845	0.0	13.145419	7
TAGTACG	240	8.185452E-11	13.104166	2
GTACTTT	11525	0.0	13.018222	13
AGAGTAC	17960	0.0	13.009745	10
TAGACAG	975	0.0	12.902564	5
GTATAGA	635	0.0	12.818897	1
CAGAGTA	18400	0.0	12.809239	9
TCTATAC	560	0.0	12.553571	3
TACTTTT	12005	0.0	12.405248	14
GCAGAGT	19230	0.0	12.400676	8
>>END_MODULE