FastQCFastQC Report
Thu 9 Feb 2017
SRR2727276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8438068
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT318710.3777049438331144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113750.1348057398921175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99580.11801279629412799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC34750.018.20719537
TATACCG4350.015.7356315
TCTTATA57400.014.34233537
TATACTG9600.014.2604175
GTCTTAG12750.013.4941181
AACGCAG165050.013.4840975
GAGTACT94550.013.48122811
CTCTTAT93500.013.37540137
AGTACTT99950.012.77138512
GTACTTT103250.012.43486613
GGGTAAG32400.012.2191361
GGTATCA118550.012.1720781
TCGTTAC9650.012.0777223
CAATACT8900.012.0561794
CTATACT7400.012.04
TACTTTT106800.011.98689114
ACGCAGA186650.011.9335656
AGAGTAC190500.011.72152210
CTCGTTA11100.011.66666722
CGCAGAG195650.011.4886287
Produced by FastQC (version 0.11.5)