##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727276.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8438068
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.170959631991586	33.0	31.0	34.0	30.0	34.0
2	32.31297472359787	34.0	31.0	34.0	30.0	34.0
3	32.39245334358529	34.0	31.0	34.0	30.0	34.0
4	35.96421621631871	37.0	35.0	37.0	35.0	37.0
5	35.844331546036365	37.0	35.0	37.0	35.0	37.0
6	35.85591974371385	37.0	35.0	37.0	35.0	37.0
7	35.80084991019271	37.0	35.0	37.0	35.0	37.0
8	35.77001903753324	37.0	35.0	37.0	35.0	37.0
9	37.50746746767151	39.0	37.0	39.0	35.0	39.0
10	37.34212203551808	39.0	37.0	39.0	34.0	39.0
11	37.448328100697935	39.0	37.0	39.0	35.0	39.0
12	37.35120610547343	39.0	37.0	39.0	34.0	39.0
13	37.407245473726924	39.0	37.0	39.0	34.0	39.0
14	38.593203562711274	40.0	38.0	41.0	34.0	41.0
15	38.55334503111376	40.0	38.0	41.0	34.0	41.0
16	38.480046024753534	40.0	38.0	41.0	34.0	41.0
17	38.43429680822672	40.0	38.0	41.0	34.0	41.0
18	38.37905110506339	40.0	38.0	41.0	34.0	41.0
19	38.38203709664345	40.0	38.0	41.0	34.0	41.0
20	38.26171927033534	40.0	38.0	41.0	34.0	41.0
21	38.182885229178055	40.0	38.0	41.0	34.0	41.0
22	38.1101631321293	40.0	38.0	41.0	33.0	41.0
23	37.99617282060301	40.0	37.0	41.0	33.0	41.0
24	37.884718990176424	40.0	37.0	41.0	33.0	41.0
25	37.79772407617478	40.0	37.0	41.0	33.0	41.0
26	37.433532533750615	39.0	37.0	41.0	32.0	41.0
27	37.22904307004874	39.0	37.0	41.0	31.0	41.0
28	37.0692844617986	39.0	36.0	41.0	31.0	41.0
29	37.026537709816985	39.0	36.0	40.0	31.0	41.0
30	36.94679990727735	39.0	36.0	40.0	30.0	41.0
31	36.739167781060786	39.0	36.0	40.0	30.0	41.0
32	36.62880638079712	39.0	36.0	40.0	30.0	41.0
33	36.446788293244374	39.0	35.0	40.0	30.0	41.0
34	36.30633872587896	39.0	35.0	40.0	29.0	41.0
35	36.157539142846446	39.0	35.0	40.0	29.0	41.0
36	36.00384400789375	39.0	35.0	40.0	28.0	41.0
37	35.767432426474876	38.0	35.0	40.0	27.0	41.0
38	35.65565838056769	38.0	35.0	40.0	26.0	41.0
39	35.487171708026054	38.0	35.0	40.0	26.0	41.0
40	35.355761413631654	38.0	34.0	40.0	25.0	41.0
41	35.22026274260885	38.0	34.0	40.0	25.0	41.0
42	35.02776464944345	38.0	34.0	40.0	24.0	41.0
43	31.60746180286767	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	24.0
11	12.0
12	15.0
13	29.0
14	61.0
15	128.0
16	223.0
17	491.0
18	926.0
19	2028.0
20	4046.0
21	7713.0
22	13511.0
23	21597.0
24	32689.0
25	46273.0
26	63197.0
27	82627.0
28	106905.0
29	135651.0
30	169635.0
31	211914.0
32	264490.0
33	332356.0
34	428031.0
35	564942.0
36	791006.0
37	1243049.0
38	2273669.0
39	1640825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.175214989971636	17.762110947671907	12.108968545880408	26.953705516476045
2	19.317834366824254	20.47360841367953	33.6704326156177	26.538124603878515
3	19.200023038449086	21.238380634050355	30.062545122888316	29.499051204612243
4	15.337373436668203	15.128012715706959	32.38914405525056	37.14546979237427
5	15.123319698300605	36.211191945834045	33.6313833925017	15.034104963363651
6	37.60499441341312	33.83005446270403	13.746653854887162	14.818297268995698
7	31.021236140784836	30.06051859264467	19.4809878280194	19.4372574385511
8	28.76938180635662	33.96509722367727	18.31021034672866	18.95531062323745
9	27.341341643608462	13.195402075451396	17.483883751588632	41.97937252935151
10	16.066592494869678	26.945658650771716	33.13330729261722	23.854441561741385
11	38.23152408821545	20.30853508172724	20.40707659620662	21.052864233850688
12	22.279140201287785	24.169158153264465	29.017329559325667	24.534372086122083
13	32.617454611648064	18.88633748862891	22.75337198041068	25.742835919312334
14	23.228077801695836	19.38724599043288	24.503393430818523	32.88128277705275
15	26.683371122394366	27.02789311486942	20.755497585466244	25.53323817726996
16	25.991553990795047	25.20927776358285	22.94756335217967	25.85160489344243
17	25.30246260162871	25.337529870581747	24.1405497087722	25.21945781901734
18	25.305259450386036	24.679274924070295	24.69589010185744	25.31957552368623
19	26.34882771743484	24.59856924594587	24.54199231388038	24.510610722738903
20	25.884467866340966	24.265933860689437	24.48504799913914	25.364550273830456
21	25.656109905727238	24.50581104584604	24.465505611000054	25.37257343742667
22	26.308605239967253	24.045042064131266	23.85706064468786	25.789292051213618
23	25.67632780394754	24.15643012120784	24.17201425729207	25.995227817552546
24	25.58854704655141	24.07260761586657	24.167356793047887	26.171488544534128
25	25.951402619651798	23.792614612728887	24.200776765487074	26.05520600213224
26	25.536248345000303	24.111680541090685	24.54404254623215	25.808028567676867
27	26.041126949913178	24.32210785691701	24.174100042806007	25.462665150363804
28	25.755172866585102	24.51114402017144	24.080204141516756	25.653478971726702
29	25.538950385325172	24.678990498772944	24.40723397820449	25.3748251376974
30	25.866952008445537	24.410267848042942	24.119514087822	25.603266055689527
31	25.774466382589	23.937197472217576	24.33305823086517	25.955277914328256
32	25.36099495761352	24.348452750084498	24.35334723540981	25.93720505689217
33	25.54557512454273	23.94434365781361	24.328531128215605	26.18155008942805
34	25.733378778175286	23.88706751355879	24.708973665535762	25.670580042730162
35	25.57250071935898	23.956455435059304	24.6325225158176	25.838521329764113
36	25.29525716076239	23.606718978799414	25.041312774440783	26.056711085997406
37	25.11055848329262	23.484771632558544	25.314467719387896	26.09020216476094
38	24.93065948271571	23.44969251255145	25.59033655571394	26.0293114490189
39	24.60448292192004	23.367126218940165	26.042371310589107	25.986019548550686
40	25.043386708900663	23.075933969719134	26.172175905669405	25.708503415710798
41	24.488555911139848	23.152385119437294	26.47385633773039	25.885202631692465
42	24.23734911830528	23.222839635802888	26.445781190670658	26.094030055221175
43	24.43639942223741	22.44503125596997	26.24995437344188	26.86861494835074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	565.0
1	603.5
2	642.0
3	1706.0
4	2770.0
5	2770.0
6	3070.0
7	3370.0
8	3369.0
9	3368.0
10	4753.5
11	6139.0
12	6139.0
13	12822.0
14	19505.0
15	33455.5
16	47406.0
17	35048.0
18	22690.0
19	22690.0
20	28257.0
21	33824.0
22	30651.0
23	27478.0
24	35373.5
25	43269.0
26	43269.0
27	54838.0
28	66407.0
29	79820.0
30	93233.0
31	111707.0
32	130181.0
33	130181.0
34	154169.5
35	178158.0
36	211136.5
37	244115.0
38	285031.0
39	325947.0
40	325947.0
41	370847.0
42	415747.0
43	463141.0
44	510535.0
45	565172.0
46	619809.0
47	619809.0
48	672024.5
49	724240.0
50	758042.5
51	791845.0
52	794604.5
53	797364.0
54	797364.0
55	764262.5
56	731161.0
57	690345.5
58	649530.0
59	604028.5
60	558527.0
61	558527.0
62	496868.0
63	435209.0
64	373682.5
65	312156.0
66	263569.5
67	214983.0
68	214983.0
69	179148.0
70	143313.0
71	121396.0
72	99479.0
73	82811.5
74	66144.0
75	66144.0
76	55174.0
77	44204.0
78	37280.0
79	30356.0
80	24626.0
81	18896.0
82	18896.0
83	14987.0
84	11078.0
85	8838.5
86	6599.0
87	5349.5
88	4100.0
89	4100.0
90	3095.0
91	2090.0
92	1546.5
93	1003.0
94	723.5
95	444.0
96	444.0
97	286.5
98	129.0
99	94.5
100	60.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8438068.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.6188934463816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42094585832544	41.790422875300685
2	10.391331018523864	10.935606792595744
3	3.6600990756467437	5.777710897939674
4	1.8197318759206764	3.8300911072021675
5	1.022572977897921	2.6903329282579915
6	0.6751574739976639	2.131562351028674
7	0.4849439137047439	1.7862048485890836
8	0.3466344737127874	1.459161794970858
9	0.2681473891141347	1.2698657006149872
>10	1.7146520314266434	16.998050955191456
>50	0.14002032276407483	5.06895459441077
>100	0.05418839281880059	4.799547936627057
>500	0.001057799197678499	0.3647339971422368
>1k	4.2741490132492023E-4	0.37421151724452945
>5k	4.4991023748099167E-5	0.21164358288724996
>10k+	4.4991023748099167E-5	0.5118981199967568
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31871	0.3777049438331144	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11375	0.1348057398921175	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9958	0.11801279629412799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.555316216934967E-5
2	0.0	0.0	0.0	0.0	3.555316216934967E-5
3	0.0	0.0	0.0	0.0	3.555316216934967E-5
4	0.0	0.0	0.0	1.1851054056449889E-5	3.555316216934967E-5
5	0.0	0.0	0.0	1.1851054056449889E-5	3.555316216934967E-5
6	0.0	0.0	0.0	1.1851054056449889E-5	4.7404216225799556E-5
7	0.0	0.0	0.0	1.1851054056449889E-5	4.7404216225799556E-5
8	0.0	0.0	0.0	1.1851054056449889E-5	4.7404216225799556E-5
9	2.3702108112899778E-5	0.0	0.0	1.1851054056449889E-5	1.1851054056449889E-4
10	3.555316216934967E-5	0.0	0.0	1.1851054056449889E-5	1.1851054056449889E-4
11	3.555316216934967E-5	0.0	0.0	2.3702108112899778E-5	1.1851054056449889E-4
12	3.555316216934967E-5	0.0	0.0	5.925527028224944E-5	1.1851054056449889E-4
13	3.555316216934967E-5	1.1851054056449889E-5	0.0	5.925527028224944E-5	1.1851054056449889E-4
14	3.555316216934967E-5	1.1851054056449889E-5	0.0	5.925527028224944E-5	1.7776581084674834E-4
15	3.555316216934967E-5	1.1851054056449889E-5	0.0	8.295737839514922E-5	1.8961686490319822E-4
16	3.555316216934967E-5	1.1851054056449889E-5	0.0	1.4221264867739867E-4	3.4368056763704676E-4
17	4.7404216225799556E-5	1.1851054056449889E-5	0.0	2.014679189596481E-4	3.4368056763704676E-4
18	4.7404216225799556E-5	1.1851054056449889E-5	0.0	2.7257424329834743E-4	4.147868919757461E-4
19	4.7404216225799556E-5	1.1851054056449889E-5	0.0	4.147868919757461E-4	4.147868919757461E-4
20	5.925527028224944E-5	1.1851054056449889E-5	0.0	5.688505947095947E-4	4.5034005414509577E-4
21	7.110632433869934E-5	1.1851054056449889E-5	0.0	9.125311623466414E-4	4.5034005414509577E-4
22	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.001398424378661087	4.5034005414509577E-4
23	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.0017895091625239332	5.451484865966949E-4
24	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.002453168189685127	5.569995406531447E-4
25	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.002867955081660873	5.569995406531447E-4
26	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.0035079120007091673	5.688505947095947E-4
27	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.007003972947361884	5.925527028224944E-4
28	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.02543236200514146	5.925527028224944E-4
29	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.06565483947273239	6.044037568789443E-4
30	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.12569227932270752	6.044037568789443E-4
31	7.110632433869934E-5	1.1851054056449889E-5	0.0	0.319504417361889	6.162548109353942E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3475	0.0	18.207195	37
TATACCG	435	0.0	15.735631	5
TCTTATA	5740	0.0	14.342335	37
TATACTG	960	0.0	14.260417	5
GTCTTAG	1275	0.0	13.494118	1
AACGCAG	16505	0.0	13.484097	5
GAGTACT	9455	0.0	13.481228	11
CTCTTAT	9350	0.0	13.375401	37
AGTACTT	9995	0.0	12.771385	12
GTACTTT	10325	0.0	12.434866	13
GGGTAAG	3240	0.0	12.219136	1
GGTATCA	11855	0.0	12.172078	1
TCGTTAC	965	0.0	12.07772	23
CAATACT	890	0.0	12.056179	4
CTATACT	740	0.0	12.0	4
TACTTTT	10680	0.0	11.986891	14
ACGCAGA	18665	0.0	11.933565	6
AGAGTAC	19050	0.0	11.721522	10
CTCGTTA	1110	0.0	11.666667	22
CGCAGAG	19565	0.0	11.488628	7
>>END_MODULE