FastQCFastQC Report
Thu 9 Feb 2017
SRR2727275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8873752
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT687180.7743962193218833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT483180.5445047371168362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT466010.5251555373645782No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203520.22935056107044688No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT147890.16666005540835488No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG125250.14114660855971634No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC106210.11969007022057862No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC102260.11523874005043189No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA101170.11401039830727747No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG99720.11237636571317296No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG95360.10746299874055529No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG93950.10587404290766747No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT91260.10284263071584601No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC89870.10127621326356652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG32900.019.3996965
CAAGACG33200.019.3358444
CTTATAC45150.017.82392137
ACGGACC37850.016.618238
GACGGAC38600.016.1515547
CGGACCA39100.016.0869569
CGCAAGA40400.015.9356442
AGACGGA39650.015.9104676
GCGCAAG41150.015.55528451
TACTTAC17450.014.84240731
CCCGTTA3750.014.80000122
TCGTTTA25250.014.50693130
GCAAGAC47350.014.4952483
TAATACC7800.014.4679494
AGAGCGA43150.014.40556215
CAGAGCG43600.014.341742514
GAGCGAA44300.014.07336316
ACGAACG24650.014.03448415
CGCTTCG22700.014.01762132
TACCGTC26550.014.005657
Produced by FastQC (version 0.11.5)